| TFome pUT5374 | |
|---|---|
| Template | Genomic DNA |
| Genbank Insert ID | KJ727515 |
| Protein Name | ZmEREB112 |
| Species | Zea mays |
| TF Family | AP2/ERF-ERF |
| Gene id | GRMZM2G039870 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT5374
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | SE0024_F |
| Temperature (ºC) | 62.2 |
| Sequence
|
caccATGACTACGGCGGCCAT |
3' Primer
| Name | SE0024_R |
| Temperature (ºC) | |
| Sequence
|
TGAGAGGATGGCGTTCCAGTC |
PCR Condition
Step 1, 98°C 3 min melt, 1 cycle; Step 2, 98&d
Amino Acid Sequence
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MTTAAMDRHWRRLQATPPPLGAVQYYRELIHSLDTPVQAAPIMVDTEPGQAMAIVGASRSKCYHLITPAQAAYYYRVVGGRLGSAPRLMKPYRCLGAPAPSTTTTKLYRGVRQRHWGKWVAEIRLPRNRTRLWLGTYDTAEEAALAYDGAAFRLRGDAARLNFPELRLGGTHLAPPLHPTVDAKLQAVTTSAPAAPALPRPESLDIESETSTTTTATAYGTGLCPSSTGSSSLAKTAPLPDMQELDFSEVPWDEGDGVALCKYPSLEIDWNAILS
TFome clones do not contain stop codon at 3'end.
CDS
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ATGACTACGGCGGCCATGGATAGGCACTGGCGCCGCCTTCAAGCTACTCCGCCGCCGCTAGGCGCCGTGCAGTACTACAGAGAGCTAATCCACTCCCTAGACACCCCGGTGCAAGCTGCTCCGATCATGGTGGACACCGAGCCGGGCCAAGCCATGGCTATCGTCGGCGCCAGCAGAAGCAAGTGCTACCACCTCATCACGCCGGCGCAGGCGGCCTACTACTACCGCGTCGTAGGCGGGCGCCTGGGATCGGCGCCGCGCCTGATGAAGCCGTACCGCTGTCTGGGCGCGCCGGCACCCTCGACGACGACGACGAAGCTGTACCGCGGCGTGCGTCAGCGGCACTGGGGCAAGTGGGTGGCGGAGATCAGGCTGCCGCGGAACCGGACGCGCCTGTGGCTCGGCACGTACGACACCGCCGAGGAGGCCGCGCTTGCCTACGACGGCGCCGCGTTCCGGCTGCGCGGCGATGCCGCCCGCCTCAACTTCCCGGAGCTTCGGCTAGGCGGTACGCACCTTGCGCCGCCGCTCCACCCGACCGTCGATGCCAAGCTCCAAGCCGTGACGACATCCGCGCCAGCGGCGCCTGCCTTGCCCCGACCCGAGAGCCTGGATATCGAGAGCGAGACCTCGACGACCACCACGGCAACGGCGTACGGCACAGGCCTCTGTCCATCATCCACCGGCTCATCGTCGTTGGCTAAGACGGCGCCACTGCCCGATATGCAGGAGCTCGACTTCTCGGAGGTGCCGTGGGATGAGGGCGACGGCGTCGCGCTCTGCAAGTACCCGTCGTTAGAAATCGACTGGAACGCCATCCTCTCA
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