| TFome pUT5380 | |
|---|---|
| Template | Genomic DNA |
| Genbank Insert ID | KJ727521 |
| Protein Name | ZmMADS25 |
| Species | Zea mays |
| TF Family | MADS |
| Gene id | GRMZM2G099408 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT5380
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | SE0050_F |
| Temperature (ºC) | 62.2 |
| Sequence
|
caccATGGCACGCAAGAAGGTGA |
3' Primer
| Name | SE0050_R |
| Temperature (ºC) | |
| Sequence
|
TGCCGATGGGAGCCCTGA |
PCR Condition
Step 1, 98°C 3 min melt, 1 cycle; Step 2, 98&d
Amino Acid Sequence
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MARKKVNLQWISNNATRRATYKRRTQGLEKKASELATLCGIKLCVVVYGEGEAQPKVWPSHEEAKQLLLKFNGMLDVGSLKKTKNQEEFLHGRSLRLREQVSKLDLENRERETLDLLHDSMCGGRLAGGEGRGKDELLSLREMVETKMRRIKARLQQLVGQGVLPPKPPLPAAPSSLQAQASCYTYNNMQSMALLEEHRHSQQDWRAVHNLAPNYSPFAGSSSEPVGCEVTTHPYNNLGACSGLPSA
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCACGCAAGAAGGTGAATCTGCAGTGGATCTCCAACAACGCTACGCGGCGCGCGACGTACAAGAGACGCACCCAGGGCCTGGAGAAGAAGGCCAGCGAGCTGGCCACGCTCTGCGGCATCAAGCTGTGCGTCGTGGTGTACGGCGAGGGCGAGGCGCAGCCGAAGGTATGGCCCTCGCACGAAGAGGCGAAGCAGCTCCTCCTGAAATTCAACGGCATGCTGGATGTCGGTAGCCTCAAGAAGACGAAGAACCAGGAGGAGTTCCTCCACGGCCGCTCCCTGAGGCTCCGCGAGCAGGTGAGCAAGCTGGACCTCGAGAACCGCGAGCGGGAGACCCTGGACCTCCTCCATGACAGCATGTGCGGAGGGCGTCTCGCCGGCGGCGAAGGCAGAGGCAAGGACGAGCTCCTTAGCCTGAGGGAGATGGTGGAGACGAAGATGAGGAGAATCAAAGCGCGGCTCCAGCAGCTCGTCGGGCAGGGAGTCCTCCCCCCAAAGCCGCCATTGCCGGCGGCACCATCATCGTTGCAGGCACAGGCTTCTTGCTACACCTACAATAACATGCAAAGCATGGCACTGCTAGAGGAACACCGGCACTCGCAGCAGGATTGGAGAGCTGTTCACAACCTAGCCCCAAACTACAGTCCCTTCGCAGGTAGTAGCAGCGAGCCAGTGGGCTGTGAAGTGACGACGCACCCTTACAATAATCTGGGTGCTTGTTCAGGGCTCCCATCGGCA
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