TFome clone pUT5385

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	SE0100_F
Temperature (ºC)	62.4
Sequence Copy to clipboard	caccATGGCATTCACCGGAGACG

3' Primer

Name	SE0100_R
Temperature (ºC)
Sequence Copy to clipboard	AAGTGACGGTGAGCTGGGGAAAG

PCR Condition

Step 1, 98°C 3 min melt, 1 cycle; Step 2, 98&d

Amino Acid Sequence

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MAFTGDADGFALDFIREHLLGAGCGGDPDPVHCTVDLDGDDITFPVLPPPQPEFEPMPFLPPPPPPPQHQHQGYIGVDPTNEYMGEPAAVFPEQEPAPPVIITFGSEPSSPVKQPLTIAVPPRPYYASAAAATAPTAAQLVAAAAEAVEDFRKYRGVRQRPWGKYAAEIRDPKRRGSRVWLGTYDTPVEAARAYDRAAFRMRGAKAILNFPNEVGTRGADFWAPPAPAKKADTAAANKRKRQQLVEDADGDDVEVVAVVVSKAAKVVDEIPASSPSQDQTSCTSRSSSVSTRETTATASSTVTSAAEETGSGAVGLPMTPSSGNWEQYWEALLGSLPLLSPQSPLPALGFPQLTVT

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGCATTCACCGGAGACGCCGATGGTTTCGCCCTCGACTTCATCCGTGAGCACCTCCTCGGCGCTGGTTGCGGCGGCGACCCCGACCCCGTGCATTGCACTGTAGATCTCGACGGCGACGACATCACCTTCCCCGTTCTGCCTCCACCTCAACCAGAATTCGAGCCCATGCCGTTCTTGCCGCCGCCGCCGCCGCCGCCGCAGCACCAGCATCAAGGGTACATCGGCGTCGACCCGACCAACGAGTACATGGGCGAGCCAGCGGCGGTGTTCCCGGAGCAGGAGCCAGCGCCACCGGTGATAATAACGTTCGGAAGCGAGCCCTCGTCCCCCGTCAAGCAGCCTCTGACCATCGCCGTGCCCCCGAGGCCGTACTACGCTTCGGCGGCAGCAGCAACGGCGCCCACCGCCGCACAGCTGGTAGCAGCGGCGGCCGAGGCCGTGGAGGACTTCCGCAAGTACCGCGGCGTCCGGCAGCGCCCCTGGGGCAAGTACGCGGCGGAGATCCGCGACCCGAAGCGGCGGGGCTCGCGCGTGTGGCTCGGCACGTACGACACGCCCGTCGAGGCCGCGCGCGCCTACGACCGTGCCGCCTTCCGCATGCGCGGCGCCAAGGCCATCCTCAACTTCCCCAACGAGGTCGGCACCCGCGGCGCCGACTTCTGGGCCCCGCCCGCTCCGGCGAAGAAGGCGGACACGGCCGCCGCGAACAAGAGGAAGCGGCAGCAGCTGGTGGAGGACGCCGACGGCGACGACGTCGAGGTGGTCGCGGTCGTGGTGAGCAAGGCAGCGAAGGTTGTTGATGAGATCCCTGCTTCGTCGCCGTCCCAGGACCAGACATCGTGCACCTCTCGGTCGTCGTCCGTGTCGACGCGCGAGACCACAGCGACAGCCTCGTCGACGGTGACGTCCGCTGCGGAGGAGACGGGATCAGGGGCTGTGGGGCTCCCGATGACGCCCTCGAGCGGGAACTGGGAGCAATACTGGGAGGCATTGCTCGGCAGCCTGCCGCTGCTCTCGCCGCAGTCGCCCCTCCCGGCGTTGGGCTTTCCCCAGCTCACCGTCACT