TFome clone pUT5396

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	B319_F
Temperature (ºC)	68.6
Sequence Copy to clipboard	caccATGGGGAGGACTCCGTGCTGCG

3' Primer

Name	B319_R
Temperature (ºC)
Sequence Copy to clipboard	CTCCTCAAACGGAGCGAAAAG

PCR Condition

PCR Reaction Mix:5 l GC buffer (NEB) 1 l primer fo

Amino Acid Sequence

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MGRTPCCDSKGIKKGPWAPEEDKLLVDYVQANGPGNWRILPKLAGTYCTVRALLLSISSPAACLSRHFSIPSWPLVGAAAARACRAEPVRQELPAAVDQLPAPGHQARALHPRGAQFHPSAPRRSRQQVVHDRGTDAGPDGQRDQELLEHAHEEASCPAARHTAQWETARLEAEARLSLLSSSAATTVTSATASSSSSTVAAEHAAPPDIFLRLWNSEVGDSFRGAAAQQGHGPEASAAGAGVPALLPELGGGGDDSSAASTYEAEAAADDGYQAFLDLAMEEFALLHGRLGAFSAFPQADVFAEAHCCLFAPFEE

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGGGAGGACTCCGTGCTGCGACAGCAAGGGCATCAAGAAGGGGCCATGGGCGCCGGAGGAGGACAAGCTGCTCGTCGACTACGTCCAGGCCAACGGACCCGGCAACTGGCGCATTCTCCCCAAGCTCGCGGGTACGTACTGTACTGTACGTGCTCTTCTCCTGTCGATATCGAGCCCGGCCGCTTGCTTGTCTCGTCATTTCTCAATACCGAGCTGGCCGCTTGTTGGTGCCGCCGCCGCGCGCGCTTGCAGGGCTGAACCGGTGCGGCAAGAGCTGCCGGCTGCGGTGGACCAACTACCTGCGCCCGGACATCAAGCGCGGGCCCTTCACCCCCGAGGAGCACAATTCCATCCTTCAGCTCCACGCCGTAGTCGGCAACAAGTGGTCCATGATCGCGGCACAGATGCCGGGCCGGACGGACAACGAGATCAAGAACTACTGGAACACGCACATGAAGAAGCCTCCTGCCCCGCCGCCCGCCACACGGCCCAGTGGGAGACCGCCAGGCTCGAGGCCGAGGCGCGCCTCTCCCTGCTCTCGTCCTCGGCCGCCACGACGGTCACCTCCGCCACCGCCTCCTCCTCGTCGTCCACCGTCGCCGCCGAGCATGCGGCGCCACCCGACATCTTCCTGCGCCTCTGGAACTCCGAGGTCGGGGATTCTTTCCGCGGCGCAGCCGCGCAGCAGGGGCACGGTCCGGAGGCGAGCGCGGCGGGCGCTGGCGTGCCGGCGCTGCTGCCTGAGCTCGGCGGTGGCGGTGACGATTCCTCGGCCGCGTCGACCTACGAGGCTGAGGCCGCGGCGGACGACGGCTACCAGGCGTTCCTGGACCTGGCGATGGAGGAGTTCGCGCTGCTGCACGGCCGGCTCGGCGCGTTCTCGGCGTTCCCGCAGGCCGACGTGTTCGCTGAGGCGCACTGCTGCCTTTTCGCTCCGTTTGAGGAG