| TFome pUT5413 | |
|---|---|
| Template | BT062696.1 |
| Genbank Insert ID | KJ727553 |
| Protein Name | ZmEREB212 |
| Species | Zea mays |
| TF Family | AP2/ERF-ERF |
| Gene id | GRMZM2G076602 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT5413
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B398_F |
| Temperature (ºC) | 63.0 |
| Sequence
|
caccATGAAGCAGATGTGCCACCTGA |
3' Primer
| Name | B398_R |
| Temperature (ºC) | |
| Sequence
|
CGTGGGCCTGGCCAGCG |
PCR Condition
PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer f
Amino Acid Sequence
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MKQMCHLSKEEFVHVLRRQSTGFPRGSSKYRGVTLHKCGRWEARMGQFLGKKYVYLGLFDTEEEAARAYDRAAIKCNGKDAVTNFDPSIYAEEEVAPAAATGGAAGDEHNLDLSLGSSAGNKRGSLDGGGGGGGDDKSSDQRVPMAFDIDWQTAARRSTKAKLLSNGDPGTAAGGLSLAIGGGGGHWPPQLQHQQQQQRLHGARNNGGGTSWPPPPHPCPPPVPAAAATAAAASSRFHPYVVTSTTQSPAGWVQKNGFHSLARPT
TFome clones do not contain stop codon at 3'end.
CDS
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ATGAAGCAGATGTGCCACCTGAGCAAGGAGGAGTTCGTTCACGTGCTCCGGCGGCAGAGCACGGGGTTCCCCCGGGGCAGCTCCAAGTACAGGGGCGTGACGCTCCACAAGTGCGGGCGGTGGGAGGCGCGCATGGGCCAGTTCCTCGGCAAGAAGTACGTCTACTTGGGCCTGTTCGACACCGAGGAGGAAGCCGCCAGGGCGTACGACCGCGCGGCCATCAAGTGCAACGGCAAGGACGCGGTGACAAACTTCGATCCCAGCATTTACGCCGAGGAGGAGGTCGCGCCGGCGGCGGCGACGGGCGGCGCCGCCGGCGACGAGCACAACCTGGACCTGTCGCTGGGAAGCTCGGCGGGGAACAAAAGGGGCAGCCTTGACGGCGGCGGCGGCGGCGGCGGCGACGACAAGTCCTCGGACCAGCGCGTCCCCATGGCGTTCGACATCGACTGGCAGACGGCCGCACGTCGGAGCACCAAGGCAAAGCTCCTGAGCAACGGCGATCCGGGGACCGCGGCGGGAGGCCTTTCCCTGGCGATCGGCGGCGGAGGCGGGCACTGGCCTCCGCAGCTGCAGCATCAGCAGCAGCAGCAGAGGCTCCACGGCGCCAGGAACAACGGCGGCGGCACGAGCTGGCCGCCGCCGCCGCACCCGTGCCCGCCGCCAGTACCGGCCGCCGCCGCGACCGCAGCTGCAGCATCATCACGATTCCATCCCTACGTTGTTACGAGTACGACGCAAAGCCCGGCCGGCTGGGTCCAGAAGAATGGGTTCCATTCGCTGGCCAGGCCCACG
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