| TFome pUT5416 | |
|---|---|
| Template | BT042027.1 |
| Genbank Insert ID | KJ727556 |
| Protein Name | ZmHB127 |
| Species | Zea mays |
| TF Family | Homeobox |
| Gene id | GRMZM2G119999 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT5416
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B480_F |
| Temperature (ºC) | 63.6 |
| Sequence
|
caccATGAAGAGGCCGACCAGCA |
3' Primer
| Name | B480_R |
| Temperature (ºC) | |
| Sequence
|
CCAACCCTCACCGCCGTACC |
PCR Condition
PCR Reaction Mix: 5 l GC buffer 5l glycerol 50% 1
Amino Acid Sequence
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MKRPTSRGSSMVSGQATTDLQEDSCRSMEPHGVDGDATAAAERDDVDAGAYDEEVDEEEELAGSRGGLGEKKRRLAADQVRALERSFEVDNKLDPERKARIARDLSLHPRQVAVWFQNRRARWKTKQIERDFAALRVRHDALRVECDALRRDKDALAAEIKELRGMVEKQMEVKLESAAEELLPVATRSAAAAAVYNKDGSTDSDSSAVFNEEASPYPYSGAALDHQQQQETSHPLGFTGFTSFLTSSFPSVYHGDSHLDQEADGFFSAAAAGDGFFAEEQSAGIGSWYGGEGW
TFome clones do not contain stop codon at 3'end.
CDS
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ATGAAGAGGCCGACCAGCAGAGGTTCTTCAATGGTCAGCGGGCAAGCAACCACAGATCTACAAGAAGATAGCTGCAGGTCCATGGAGCCGCACGGAGTGGACGGCGATGCTACGGCCGCGGCTGAGCGCGACGACGTGGACGCCGGGGCGTACGACGAGGAGGTGGACGAAGAAGAGGAGCTCGCAGGGTCCCGCGGCGGCCTCGGGGAGAAGAAGCGGCGGCTGGCGGCGGATCAGGTGCGCGCCCTGGAGCGGAGCTTCGAGGTAGACAACAAGCTGGACCCAGAGCGCAAGGCCCGGATCGCGCGCGACCTCAGCCTCCACCCGCGCCAGGTCGCCGTCTGGTTTCAGAACCGCCGCGCGCGCTGGAAGACCAAGCAGATCGAGCGCGACTTCGCCGCGCTCCGCGTGCGCCACGACGCACTCCGCGTCGAGTGCGACGCGCTCCGCCGCGACAAAGACGCCCTCGCCGCCGAGATAAAGGAGCTGAGGGGCATGGTGGAAAAGCAGATGGAGGTGAAGCTGGAGTCCGCGGCGGAGGAGCTGCTGCCGGTGGCGACGAGGTCAGCAGCAGCAGCCGCTGTGTATAACAAGGACGGGTCGACGGACAGCGACTCGAGCGCCGTGTTCAACGAGGAGGCGTCGCCCTACCCTTACTCCGGCGCGGCCTTAGACCATCAGCAGCAGCAAGAAACCAGCCACCCGCTGGGCTTCACGGGTTTCACCTCCTTCTTGACGTCCTCTTTCCCTTCCGTATACCATGGGGATTCGCATTTGGACCAGGAAGCGGACGGCTTCTTCAGCGCCGCCGCCGCCGGCGACGGATTCTTCGCCGAGGAGCAGAGCGCAGGCATCGGCAGCTGGTACGGCGGTGAGGGTTGG
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