TFome clone pUT5421

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	B536_F
Temperature (ºC)	64.8
Sequence Copy to clipboard	caccATGGGGATCTACGAGCGGCA

3' Primer

Name	B536_R
Temperature (ºC)
Sequence Copy to clipboard	TGCGGGCTCCCTGGGGC

PCR Condition

PCR Reaction Mix:5 l GC buffer (NEB) 1 l primer fo

Amino Acid Sequence

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MGIYERQRHLVPTGVWGEPFRLDADAVAMPLPLAAFPSVTVATPAPLDVVEPEAEEIKLGKRLLQAQQDDVPRMQEAPPSSDSFSHDDDDARPRDKVQRRLAQNREAARKSRLRKKAYIRNLETSRVKLAQLEQELIMARRQQHGAYGVGGGVAPPAAPVDPRVAAFELEYAHWVEEQSRQATELRAALQSHAPDVQLRVLVDAALAHYGALFQAKARAARSDAFFVLSGVWRSPAERFFLWIAGFRPSDLLKVLEPQLSPLMDHQASEVRKLQNTARQLEDALSQGMSKLQQTLVDTLMTVDVSPDGAGGGYAGQQMACAVGKLADLVDFVDKADHLRQQTLRNMHKILTPRQAARGLLALADYGQRLRALSSLWAARPREPA

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGGGATCTACGAGCGGCAGCGCCACCTGGTGCCCACCGGCGTGTGGGGGGAACCGTTCCGGCTCGACGCCGACGCCGTGGCCATGCCCCTGCCCCTGGCCGCGTTCCCCTCGGTCACCGTGGCCACGCCGGCGCCGCTCGACGTCGTCGAGCCGGAAGCAGAGGAGATCAAGCTCGGCAAACGACTGTTGCAGGCGCAGCAGGACGACGTGCCGCGGATGCAAGAAGCGCCGCCGTCGTCGGATAGCTTCAGCCACGACGATGACGACGCTAGGCCGAGAGACAAGGTTCAGAGACGGCTCGCGCAGAACAGAGAGGCAGCTCGGAAGAGCCGGCTACGGAAGAAGGCGTACATACGGAACCTCGAGACGAGCCGCGTGAAGCTGGCGCAGCTGGAGCAGGAGCTCATAATGGCCAGGCGCCAGCAGCACGGCGCGTACGGCGTGGGAGGAGGAGTCGCACCGCCGGCGGCGCCCGTGGACCCGCGCGTCGCTGCGTTCGAGCTGGAGTACGCGCACTGGGTGGAGGAGCAGAGCAGGCAGGCGACGGAGCTGCGGGCGGCGCTGCAGTCGCACGCGCCGGACGTCCAGCTGCGGGTCCTCGTCGACGCCGCGCTGGCGCACTACGGCGCGCTGTTCCAGGCCAAGGCGAGGGCAGCCCGGTCCGACGCCTTCTTCGTGCTGTCCGGCGTGTGGCGGTCCCCCGCGGAGCGCTTCTTCCTCTGGATCGCCGGCTTCCGCCCCTCCGACCTGCTCAAGGTGCTGGAGCCGCAGCTGAGCCCGCTCATGGACCACCAGGCGTCCGAGGTGCGCAAGCTGCAGAACACGGCGCGGCAGCTGGAGGACGCGCTGTCGCAGGGCATGAGCAAGCTGCAGCAGACGCTGGTCGACACCCTCATGACCGTCGACGTCTCCCCGGACGGCGCCGGGGGCGGATACGCGGGGCAGCAGATGGCCTGCGCCGTGGGCAAGCTCGCCGACCTCGTCGACTTCGTGGACAAGGCGGACCACCTCCGGCAGCAGACGTTGCGGAACATGCACAAGATCCTGACGCCGCGGCAGGCAGCGCGCGGCCTGCTCGCGCTCGCGGACTACGGTCAGCGCCTGCGCGCGCTCAGCTCGCTCTGGGCGGCGCGCCCCAGGGAGCCCGCA