| TFome pUT5425 | |
|---|---|
| Template | BT069410.1 |
| Genbank Insert ID | KJ727565 |
| Protein Name | ZmMADS56 |
| Species | Zea mays |
| TF Family | MADS |
| Gene id | GRMZM2G026223 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.G335A p.G112D Present in template FlcDNA. Present in k-box functional domain. |
| Request Information | Deposited to ABRC |
TFome clone pUT5425
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B634_F |
| Temperature (ºC) | 63.7 |
| Sequence
|
caccATGGTGCGGGGCAAGAC |
3' Primer
| Name | B634_R |
| Temperature (ºC) | |
| Sequence
|
AAGCCTGTTTTCTTGGGTTGTTGG |
PCR Condition
PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer f
Amino Acid Sequence
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MVRGKTQMKRIENPASRQVTFSKRRNGLLKKAFELSVLCDAEVALVVFSPRGKLYEFASGSAQKTIERYRTYTKDNVSNKTAHQDIEQVKADAEGLAKKLEALEAYKRKLLDERLEECSFEELHSLEVKLEKSLHCIRGRKTQLLEEQLHKLKEKEMNLRKSNEDLREKVMGAKTFCRRCTAASGMPTTQENRL
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGTGCGGGGCAAGACGCAGATGAAGCGGATAGAGAACCCGGCCAGTCGCCAGGTCACCTTCTCCAAGCGCCGCAACGGCCTGCTCAAGAAGGCGTTCGAGCTCTCCGTCCTCTGCGACGCCGAGGTCGCGCTCGTCGTCTTCTCCCCGCGCGGCAAGCTCTACGAATTCGCCAGCGGAAGTGCCCAGAAAACAATTGAACGTTATAGAACGTACACAAAGGATAATGTCAGCAACAAGACAGCACATCAGGACATTGAGCAAGTAAAAGCTGATGCTGAGGGCCTGGCAAAGAAACTGGAAGCACTTGAAGCTTACAAAAGAAAACTTTTGGATGAGAGGTTGGAAGAATGCTCCTTTGAAGAACTGCACAGTTTGGAAGTGAAACTCGAAAAGAGCCTTCATTGCATCAGGGGAAGAAAGACTCAGCTGCTGGAGGAGCAACTCCATAAGCTGAAGGAGAAGGAGATGAATCTGCGCAAGAGCAACGAAGATTTACGTGAAAAGGTAATGGGGGCGAAAACATTTTGCAGACGCTGCACTGCGGCAAGCGGCATGCCAACAACCCAAGAAAACAGGCTT
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