| TFome pUT5442 | |
|---|---|
| Template | BT066413.1 |
| Genbank Insert ID | KJ727582 |
| Protein Name | ZmMYB185 |
| Species | Zea mays |
| TF Family | MYB |
| Gene id | GRMZM2G129817 |
| transcript | T02 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT5442
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C163_F |
| Temperature (ºC) | 67.7 |
| Sequence
|
caccATGGGGCGGTCGCGAGG |
3' Primer
| Name | C163_R |
| Temperature (ºC) | |
| Sequence
|
GACGGTTGGGGCCTCCTCATGGTT |
PCR Condition
PCR Reaction Mix: 5 l GC buffer (Thermo Fisher?Fin
Amino Acid Sequence
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MGRSRGVLSSGDDDTGHRSKRRRVSSGGDATDSISASIGGAGEGGGKKALYHCNYCNKDISGKIRIKCSKCPDFDLCVECFSVGAEVTPHRSNHPYKVMDNLSFPLICPDWNADEEILLLEGIEMYGLGNWLEVAEHVGTKSKLQCIDHYTSAYMNSPCYPLPDMSHVNGKNRKELLAMAKVQGESKKGTLLLPGELTPKVESQFSPSRVKVEDALGEGPAGRSPSHMAVGANKKASNVGHIKDGATVSKVEDVHVDRSVGVKKPRYSADEGPSLTELSGYNAKRHEFDPEYDNDAEQALAEMEFKETDSETDRELKLRVLRIYLSRLDERKRRKEFILERNLLFPNPLEKDLTSEDRELYHRYKVFMRFLSKEEHEALVRSVIEERKIRRRIQELQECRSAGCRTLAEAKIHIEQKRKKEYELNAQKAKDSSQLNANNKSVQKMNRPMKIESDGNLDPKKGGAGLDSPKTTGPTSVKQWDDWDIVGLPGAELLSASEKLLCCQNRLLPSHYLRMQEVLMQEIFKGSVLKKEDAHVLFKVDPTKVDSVYDMVSKKLGNHEEAPTV
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGGGCGGTCGCGAGGGGTGCTGAGTTCCGGCGACGACGACACCGGGCACAGGTCGAAGCGGAGGAGAGTCTCGTCGGGCGGGGATGCGACGGACTCCATTTCAGCCTCCATCGGGGGAGCTGGAGAGGGAGGGGGCAAGAAGGCGCTCTATCACTGCAACTACTGCAACAAGGACATCTCCGGGAAGATACGGATCAAGTGCTCCAAGTGCCCTGACTTCGACCTTTGCGTGGAGTGTTTCTCTGTCGGCGCTGAAGTCACCCCGCACCGCAGCAACCATCCTTACAAAGTCATGGACAACTTGTCTTTCCCACTTATTTGCCCGGATTGGAATGCAGATGAAGAAATTCTCCTCCTTGAGGGAATTGAAATGTATGGTCTGGGAAACTGGCTTGAAGTTGCAGAGCATGTTGGTACCAAGTCTAAGTTACAGTGTATTGATCATTACACATCAGCATACATGAACTCACCTTGTTATCCCCTCCCGGATATGTCTCATGTTAATGGCAAGAATAGGAAGGAACTTCTAGCTATGGCTAAAGTACAGGGTGAGAGTAAAAAAGGGACTTTGCTGTTACCTGGAGAACTCACTCCTAAGGTTGAATCTCAATTTTCTCCCTCCAGGGTCAAGGTGGAAGATGCACTTGGAGAAGGTCCAGCAGGTCGATCACCTTCACACATGGCTGTTGGTGCAAATAAAAAAGCTTCAAATGTGGGACATATTAAGGATGGCGCTACTGTATCAAAAGTCGAAGATGTTCATGTAGATAGAAGTGTTGGTGTGAAGAAGCCCAGATATTCTGCAGATGAAGGCCCTTCGTTGACTGAACTGAGTGGATACAATGCAAAGAGACATGAGTTTGACCCAGAATACGATAATGATGCCGAACAAGCTCTTGCTGAGATGGAATTTAAAGAAACTGATTCGGAAACTGATCGTGAACTGAAACTCCGTGTGCTGCGTATTTACCTCTCCAGGCTTGATGAAAGAAAAAGAAGAAAGGAGTTCATATTGGAAAGAAATTTGTTGTTCCCTAATCCCTTGGAGAAGGATCTTACGAGTGAAGACAGGGAACTTTACCATCGCTATAAAGTCTTCATGCGTTTTCTTTCTAAGGAGGAACATGAAGCCCTCGTTAGGAGTGTTATTGAGGAGCGAAAAATTCGGAGGAGAATTCAAGAACTCCAGGAATGCCGTTCTGCTGGATGCCGCACACTGGCTGAAGCAAAGATACACATAGAGCAAAAGAGGAAAAAAGAATACGAGCTGAATGCGCAAAAAGCTAAGGATAGCAGTCAACTTAATGCAAATAATAAATCAGTACAAAAGATGAATCGACCTATGAAAATTGAGTCCGATGGGAATTTGGATCCAAAGAAAGGTGGTGCTGGCTTGGATTCTCCTAAGACAACAGGACCTACAAGTGTTAAGCAGTGGGATGACTGGGATATAGTTGGTCTTCCTGGGGCAGAGCTATTAAGTGCTAGCGAAAAGCTTCTATGCTGTCAGAACAGATTGCTACCCAGCCATTACCTGAGAATGCAGGAGGTGCTGATGCAGGAGATATTCAAGGGTAGCGTCCTAAAGAAGGAAGACGCCCACGTCTTATTTAAGGTCGATCCTACCAAAGTAGATAGTGTTTATGATATGGTATCTAAAAAACTGGGCAACCATGAGGAGGCCCCAACCGTC
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