| TFome pUT5444 | |
|---|---|
| Template | BT066238.1 |
| Genbank Insert ID | KJ727584 |
| Protein Name | ZmWRKY113 |
| Species | Zea mays |
| TF Family | WRKY |
| Gene id | GRMZM2G147880 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT5444
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C176_F |
| Temperature (ºC) | 63.6 |
| Sequence
|
caccATGGAGGCGATGGAGGAGG |
3' Primer
| Name | C176_R |
| Temperature (ºC) | |
| Sequence
|
GGCTGGCTGGGCTAGAACTCGG |
PCR Condition
PCR Reaction Mix: 5 l GC buffer 1 l primer forward
Amino Acid Sequence
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MEAMEEANRTAVESCHRVLALLSNPHGQLVPSRELVATTGEAVARFGSLAAKLSSSNGNGLQGHARVRKTKKPLPIFYSNLFLESSSAVAAAATVAKTPSPSPTTGLQLFPRYHQMEGSSCKDPVRIPAQFPKRLLLENLAAGLEGSSPPAPPVRMVQPVSVAPPAGMPTPALPAAHLHFIQQQQSYQRFQLMQQMKIQSEMVKRSNLGEQGGSLSGGGGGGKGVNLKFDSSNCTASSSRSFLSSLSMEGSLASLDGSRASRPFQLVSGSQTSSTPELGLVQRRRCAGKEDGSGQCATGSRCHCSKKRKLRIRRSIKVPAVSNKVADIPADEFSWRKYGQKPIKGSPHPRGYYKCSSVRGCPARKHVERCVDDPSMLIVTYEGDHNHSRVLAQPA
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGAGGCGATGGAGGAGGCCAACAGGACGGCAGTGGAGAGCTGCCACCGGGTGCTGGCGCTCCTCTCCAACCCGCACGGCCAGCTCGTCCCCAGCAGGGAGCTCGTGGCCACCACCGGGGAGGCCGTAGCCAGGTTCGGCTCCCTGGCGGCCAAGCTCTCCAGCTCCAACGGCAATGGCCTGCAGGGCCACGCTAGGGTCAGGAAGACCAAGAAGCCCCTGCCCATCTTCTACAGCAACCTCTTCCTCGAGAGCAGCTCTGCGGTGGCCGCCGCCGCGACTGTGGCCAAGACGCCCAGTCCGAGCCCGACCACTGGCCTCCAGCTGTTCCCGAGGTACCACCAGATGGAGGGCTCGTCGTGTAAGGATCCTGTCAGGATCCCTGCGCAGTTCCCCAAGAGGTTGCTGCTGGAGAACCTGGCTGCCGGTCTGGAGGGGTCGTCGCCGCCTGCCCCTCCGGTCCGGATGGTGCAGCCGGTGTCCGTTGCGCCTCCCGCAGGGATGCCCACCCCGGCATTGCCCGCCGCACACCTCCATTTCATCCAGCAGCAGCAGAGCTACCAGAGGTTTCAGCTCATGCAGCAGATGAAGATTCAGAGCGAGATGGTTAAGAGGAGCAATCTTGGTGAGCAGGGTGGTAGCTTAAGTGGCGGCGGGGGCGGTGGTAAGGGTGTGAACCTCAAGTTTGACAGCTCCAATTGCACGGCGTCATCGTCTCGCTCGTTCCTTTCGTCTCTGAGCATGGAAGGAAGTCTTGCGAGCTTGGATGGAAGCCGGGCCAGCAGGCCGTTCCAGCTAGTTAGTGGCTCTCAGACATCTAGCACACCGGAGCTGGGCCTGGTGCAAAGGAGAAGGTGTGCTGGGAAGGAGGATGGGAGTGGTCAGTGCGCAACCGGGAGCCGGTGCCATTGTTCAAAGAAAAGGAAGCTAAGGATAAGGAGGTCCATCAAAGTCCCTGCAGTAAGCAACAAGGTTGCAGACATCCCAGCTGATGAGTTCTCGTGGAGGAAGTATGGGCAGAAGCCAATCAAGGGATCCCCACATCCTAGGGGTTACTACAAGTGTAGCAGCGTGAGAGGCTGCCCCGCGAGGAAGCACGTCGAGAGGTGCGTGGACGACCCTTCGATGCTGATCGTTACCTACGAGGGTGACCACAACCACAGCCGAGTTCTAGCCCAGCCAGCC
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