| TFome pUT5447 | |
|---|---|
| Template | BT034337.1 |
| Genbank Insert ID | KJ727587 |
| Protein Name | ZmEREB208 |
| Species | Zea mays |
| TF Family | AP2/ERF-AP2 |
| Gene id | GRMZM2G160971 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT5447
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C183_F |
| Temperature (ºC) | 68.9 |
| Sequence
|
caccATGTCGCGCCCGCGCAC |
3' Primer
| Name | C183_R |
| Temperature (ºC) | |
| Sequence
|
GAAAATCGCGGGAAGCGGGTTGA |
PCR Condition
PCR Reaction Mix:5 l GC buffer 1 l primer forward
Amino Acid Sequence
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MSRPRTVRIFWDDPDLTDSSSEEEGCGTRRVGRMVRELPPMAGAAAAAPVPDQCNAGDGDRGRRLGVAAPGACSGARRRLAKTGPSTKFRGVRRRPWGKFAAEIRDPWRGVRVWLGTFDTAEEAARVYDTAAIQLRGANATTNFSAAANSGSRAGQRDPATPGYESGTESSPAASSPTSVLRKVPSLSSLAEDDSCAAPCEPAAGCRSLAVLEEEELGQFVPFEDAPVYATSGFWDFEPDAGFLYAEPSSPEASWNAAAAAEPASSDDAPSWAASPVQENDYFQGLRDLFPLNPLPAIF
TFome clones do not contain stop codon at 3'end.
CDS
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ATGTCGCGCCCGCGCACGGTGCGGATCTTCTGGGACGATCCTGACCTGACGGACTCCTCCAGCGAGGAGGAGGGCTGCGGGACCCGGAGGGTGGGCAGGATGGTGCGGGAGCTGCCACCCATGGCGGGTGCGGCGGCGGCTGCTCCCGTGCCGGACCAGTGCAACGCCGGGGACGGCGACCGCGGGAGGAGACTCGGCGTTGCTGCTCCCGGTGCGTGCAGCGGGGCGCGGAGACGGCTGGCCAAGACTGGCCCGAGCACCAAGTTCCGCGGCGTCCGGAGGCGGCCGTGGGGCAAGTTCGCCGCGGAGATCCGTGACCCGTGGCGCGGCGTGCGCGTGTGGCTCGGCACCTTCGACACCGCGGAGGAGGCCGCCCGCGTCTACGACACCGCCGCCATCCAGCTCCGCGGGGCCAACGCCACCACCAACTTCTCCGCCGCCGCTAACTCCGGCTCCAGGGCCGGCCAGCGGGACCCGGCGACCCCCGGCTACGAGTCCGGCACGGAGTCCTCGCCGGCGGCGTCGTCGCCGACGTCCGTGCTCCGCAAGGTCCCCTCCCTGTCCTCCCTCGCCGAGGACGACTCCTGCGCGGCCCCGTGCGAGCCAGCCGCGGGGTGCCGCAGCCTGGCCGTCCTCGAGGAGGAGGAGCTCGGCCAGTTCGTGCCGTTCGAGGACGCGCCGGTCTACGCCACCAGCGGCTTCTGGGACTTCGAGCCCGACGCGGGGTTCCTCTACGCCGAACCGTCGTCGCCGGAGGCGTCGTGGAACGCCGCCGCCGCCGCCGAGCCGGCCTCGTCCGACGACGCGCCCAGCTGGGCCGCGTCGCCGGTGCAGGAGAACGACTACTTCCAGGGCCTCCGCGATCTCTTCCCGCTCAACCCGCTTCCCGCGATTTTC
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