TFome clone pUT5458

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	C244_F
Temperature (ºC)	65.7
Sequence Copy to clipboard	caccATGGACCCGCGCTTCCC

3' Primer

Name	C244_R
Temperature (ºC)
Sequence Copy to clipboard	CATGAAATCCATGGAGTCACTCAAGGG

PCR Condition

PCR Reaction Mix:5 l GC buffer (Thermo Fisher/ Fin

Amino Acid Sequence

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MDPRFPPPAPSSCFGGGGELPARGHHRRAHSETFIRLPDADLLLDPDADFGFSDIDFPSLSDDSPAASDPTPQPQAPPLQPPLQQAASPASAAPPRPPSGAHMRSLSLDAAFFDGLSLQGGGGGGGVRGGMAGHKRSGSMDGDTSPFEGESAPPSVLPDYAKKAVPDDKLAELALLDPKRAKRILANRQSAARSKERKIKYTSELERKVQTLQTEATTLSAQLTLLQRDTSGLTTENRELKLRLQAMEEQAKLRDALNDALREEVQRLKIAAGQVPNMNGNPFNGGLSQQQQMPSYFSQPQQMQYFSGHQGQHHHPKHDPQNSSNGGGQPLSDSMDFM

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGACCCGCGCTTCCCGCCGCCGGCGCCGTCGTCCTGCTTCGGCGGGGGCGGGGAGCTCCCAGCACGGGGGCACCACCGGCGGGCGCACTCGGAGACGTTCATCCGGCTGCCCGACGCAGACCTGCTGCTGGACCCGGACGCCGACTTCGGCTTCTCCGACATCGACTTCCCCTCTCTCTCCGACGACTCGCCCGCGGCGTCCGACCCGACGCCGCAGCCCCAGGCCCCGCCGCTCCAGCCGCCGCTGCAGCAGGCGGCTTCCCCGGCGTCCGCGGCGCCGCCGAGGCCGCCGAGCGGGGCGCACATGCGGAGCCTCTCCCTCGACGCCGCCTTCTTCGACGGCCTCTCGCTGCAGGGAGGCGGCGGCGGCGGGGGGGTAAGAGGGGGCATGGCGGGGCACAAGAGGAGCGGCTCCATGGACGGGGACACCTCGCCGTTCGAGGGCGAGTCGGCGCCGCCGTCCGTGCTGCCGGACTACGCCAAGAAGGCCGTGCCCGACGACAAGCTCGCCGAGCTCGCGCTCCTCGATCCCAAGCGCGCCAAGAGGATCCTGGCGAACCGGCAGTCCGCGGCGAGGTCCAAGGAGAGGAAGATCAAGTACACCAGTGAGCTGGAGAGGAAGGTGCAGACGCTGCAGACAGAGGCCACCACGCTTTCGGCACAGCTCACGCTTCTTCAGAGGGACACCTCTGGTTTAACCACTGAGAACAGGGAGCTGAAACTCCGGTTGCAGGCCATGGAAGAACAAGCTAAACTTCGAGATGCTCTAAATGATGCCTTGAGGGAAGAAGTCCAGCGGCTTAAGATAGCCGCAGGGCAGGTCCCCAACATGAACGGGAATCCCTTCAATGGAGGACTATCACAGCAGCAGCAGATGCCAAGCTATTTCTCACAACCACAGCAGATGCAATACTTCAGCGGCCACCAGGGCCAGCATCACCATCCGAAGCATGACCCCCAGAATTCTTCAAACGGCGGTGGCCAGCCCTTGAGTGACTCCATGGATTTCATG