TFome clone pUT5466

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	C298_F
Temperature (ºC)	62.9
Sequence Copy to clipboard	caccATGGGAACAATGGTTGCAGCG

3' Primer

Name	C298_R
Temperature (ºC)
Sequence Copy to clipboard	CACGAAGGACCAGTTGACGAAGCAGA

PCR Condition

PCR Reaction Mix: 5 l GC buffer (Thermo Fisher/ Fi

Amino Acid Sequence

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MGTMVAAVALRGGSSDSGGFDKVPGMDSGKYVRYTPEQVEVLERLYIDCPKPSSSRRQQLLRECPILSNIEPKQIKVWFQNRRCRDKQRKESSRLQAVNRKLTAMNKLLMEENERLQKQVSQLVHENAHMRQQLQNTSLANDTSCESNVTTPPNPIRDASNPSGLLAIAEETFTEFLSKATGTAIDWVQMPGMKPGPDSVGIVAISHGCRGVAARACGLVNLEPTKGIEILKDRPSWFRDCRSLEVFTRFPAGNGGTIELIYMQMYAPTTLVPARDFWTLRYTTTMEDGSLVVCERSLSGSGGGPNAASTQQFVRAEMLPSGYLVRPCEGGGSIVHIVDHLDLEAWSVPEVLRPLYESSRVVAQKMTTVALRHLRQIAQETSGEVVYALGRQPAVLRTFSQRLSRGFNDAISGFNDDGWSVMGGDGIEDVVIACNSTKKIRNTSNAGITFGAPGGIICAKASMLLQSVPPAVLVRFLREHRSEWADYNIDAYLASSLKTSACSLPGLRPMRFSEGQMIMPLAHTVENEEILEVVRLEGQPLTHDEALLSRDIHLLQLCTGIDEKSVGSSFQLVFAPIDEHFPDDAPLISSGFRVIPLDVKTDGVSSGRTLDLASSLDVGSAAPQASGESPDDCSLRSVLTIAFQFPYEMHLQDSVAAMARQYVRSVISAVQRVSMAISPSQSGLNAGHRMLSGFPEAATLARWVCQSYHYHLGMELLNQSDGAGEALLKMLWHHPDAVLCCSFKEKPMFTFANKAGLDMLETSLVALQDLTLDKIFDESGRKALFSDISKLMEQGYAYLPSGVCMSGMGRHVSFDQAVAWKVLGEDSNIHCLAFCFVNWSFV

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGGAACAATGGTTGCAGCGGTGGCGTTGCGAGGGGGAAGCAGCGATAGCGGAGGATTTGATAAGGTTCCCGGGATGGACTCGGGAAAATACGTGCGCTACACCCCGGAGCAAGTTGAGGTGCTCGAGCGGCTCTACATAGATTGCCCCAAGCCAAGCTCCTCACGGCGGCAGCAACTGCTGCGCGAGTGTCCTATACTCTCCAACATTGAGCCAAAGCAGATCAAGGTCTGGTTCCAGAACCGGAGGTGCCGCGATAAGCAGCGGAAGGAGTCTTCGCGGCTTCAGGCTGTGAACAGAAAGTTGACGGCAATGAACAAGCTTCTAATGGAAGAGAATGAGCGGCTTCAGAAGCAAGTCTCTCAGTTGGTTCATGAAAACGCGCACATGCGGCAGCAGCTGCAGAATACTTCATTGGCCAATGACACAAGCTGTGAATCAAATGTCACTACCCCTCCAAACCCTATAAGGGACGCAAGTAACCCTTCTGGACTCCTTGCGATTGCAGAGGAGACCTTCACAGAGTTCCTCTCAAAGGCTACTGGGACAGCTATTGATTGGGTCCAGATGCCTGGGATGAAGCCTGGTCCGGATTCAGTTGGTATCGTGGCCATTTCGCATGGTTGCCGTGGCGTTGCTGCCCGCGCCTGTGGTTTGGTGAATCTAGAACCAACAAAAGGCATAGAGATCTTGAAAGATCGTCCCTCTTGGTTCCGTGATTGCCGAAGTCTTGAAGTGTTTACAAGGTTTCCAGCTGGAAATGGGGGAACAATTGAACTTATTTACATGCAGATGTATGCCCCAACAACTTTAGTCCCTGCACGTGATTTTTGGACACTACGATACACGACAACAATGGAAGATGGCAGCCTTGTGGTCTGTGAGAGATCCTTGAGTGGTTCTGGTGGTGGTCCAAATGCAGCCTCTACACAACAATTTGTTAGGGCTGAGATGCTTCCAAGTGGGTATTTAGTTCGCCCATGCGAAGGTGGAGGATCAATTGTGCATATAGTGGACCATCTAGATCTCGAAGCATGGAGTGTTCCTGAAGTGCTTCGACCACTGTATGAGTCTTCTAGAGTTGTTGCTCAGAAAATGACTACTGTGGCACTGCGCCACCTTAGACAAATTGCTCAAGAAACAAGTGGAGAAGTAGTGTACGCCTTGGGAAGGCAACCTGCAGTCCTACGGACCTTTAGTCAAAGACTAAGCAGGGGGTTTAATGATGCCATTAGCGGTTTCAATGATGATGGCTGGTCTGTAATGGGGGGAGATGGCATTGAAGACGTTGTTATTGCTTGCAACTCAACCAAGAAAATTAGGAATACCAGCAATGCTGGGATTACATTTGGAGCCCCAGGAGGCATTATTTGTGCGAAGGCATCCATGTTACTGCAGAGCGTCCCACCGGCAGTACTGGTACGATTTCTGAGGGAGCATAGATCTGAATGGGCTGATTACAATATTGATGCGTATTTGGCTTCATCATTGAAGACCAGTGCGTGCTCACTTCCTGGGTTGCGACCCATGAGATTTTCTGAGGGGCAGATGATCATGCCACTTGCTCACACAGTTGAGAACGAGGAGATTCTTGAAGTTGTCCGCCTTGAAGGTCAGCCTCTTACTCATGATGAAGCTCTTCTTTCAAGGGACATCCACCTTCTCCAGCTTTGCACTGGAATAGATGAGAAATCTGTGGGTTCCTCCTTCCAGCTTGTGTTTGCACCAATTGATGAGCATTTCCCAGATGATGCTCCATTGATTTCTTCTGGCTTTCGTGTCATACCACTTGATGTGAAAACAGATGGTGTATCCTCTGGTAGGACGCTAGATTTGGCATCTAGTCTTGATGTGGGCTCTGCTGCACCCCAAGCCTCAGGGGAATCTCCAGATGACTGCAGTTTGAGATCTGTGCTGACAATCGCCTTTCAATTCCCGTATGAGATGCACCTTCAGGACAGCGTTGCAGCAATGGCCCGTCAATATGTTCGTAGTGTCATTTCTGCTGTGCAAAGAGTGTCGATGGCTATATCTCCCTCCCAATCTGGTCTAAATGCTGGGCATAGGATGCTTTCTGGCTTCCCTGAAGCTGCCACACTTGCTAGATGGGTTTGCCAGAGTTATCACTACCATCTAGGTATGGAATTACTTAATCAATCAGATGGAGCTGGTGAAGCATTGTTGAAAATGCTCTGGCATCATCCAGATGCTGTTCTGTGCTGCTCCTTTAAGGAGAAACCTATGTTTACGTTTGCAAACAAGGCAGGGCTGGACATGTTAGAAACATCTCTTGTTGCCCTGCAAGACCTGACGCTAGACAAGATCTTCGACGAGTCAGGAAGGAAAGCACTATTCTCAGACATCTCGAAACTAATGGAACAGGGCTACGCGTACCTGCCGTCAGGCGTGTGCATGTCAGGAATGGGCCGCCATGTTTCTTTCGACCAAGCTGTAGCGTGGAAGGTGCTCGGCGAGGATAGCAACATCCACTGCCTGGCGTTCTGCTTCGTCAACTGGTCCTTCGTG