| TFome pUT5580 | |
|---|---|
| Template | BT066152 |
| Genbank Insert ID | KJ727653 |
| Protein Name | ZmSIG7 |
| Species | Zea mays |
| TF Family | Sigma70-like |
| Gene id | GRMZM5G830932 |
| transcript | T02 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT5580
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C450F |
| Temperature (ºC) | 61.5 |
| Sequence
|
caccATGCTTGCTTGCAGATGTTGC |
3' Primer
| Name | C450R |
| Temperature (ºC) | |
| Sequence
|
GACGAAGTCGTGGAGGTCGCGG |
PCR Condition
Step 1, 98°C 40 sec melt, 1 cycle; Step 2, 98&
Amino Acid Sequence
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MLACRCCCYRLGGDYSVLCSNDARNDDGTPRQCAWLITMNGGRAISSESLAALAWHFLLRRRSRDGATALASSSGVLQIAEDKSSAANLTKHKVSAERKLLNDALDRTGVRYETESWMDEETSSWMDRDLTDSDLRYRLLMQNLEELETSLVGKDLEMDILEQLGALGSFNASVARGVSALHGRPDGSLLDDPEPETTPLDAGQEDSQVVVVRSGKSQERKLRRMRASSDEKGSRTSVKVNPPRRPKKSRKASSSQFISEWKSHPGQRRIIVREQSALLSTIKECAGLEKIREKMVKEGQEVSYHRWAEAAGVDEAELVSRLQAGYCCRDRLLVTTEWLVRCIARTYTGMGTALDDLLQAGKMGVLDGAEKFDSRKGCRFSTYVKYWIRKGMLALLAENSGVTLLPARMESIMRKVKEARRGIRYRQGRNPSDSEVAAAVGVSVANVRLARKCSRRAVSLYSEVAIGQDVKFAEVIPDESASPDEAALFRGQLRERLLLVLGRLPAREGRVLRLRHGLEDGRCRSLEEIGGIYGVSKEWIRKIEKSAMARLRDDDAVRRDLHDFV
TFome clones do not contain stop codon at 3'end.
CDS
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ATGCTTGCTTGCAGATGTTGCTGTTATCGCCTTGGGGGTGATTATTCTGTCCTGTGCTCTAATGATGCTCGGAATGATGATGGAACTCCCCGTCAGTGCGCCTGGTTAATCACAATGAACGGAGGAAGGGCAATCTCCTCTGAATCCCTCGCGGCACTGGCATGGCATTTCCTGCTAAGGCGTCGCAGCAGGGACGGTGCCACAGCTCTCGCATCTTCATCCGGGGTGCTTCAAATCGCAGAGGACAAATCATCTGCGGCCAACTTGACAAAGCACAAGGTGAGTGCAGAAAGGAAGCTCCTCAACGACGCTCTTGACAGAACTGGTGTGCGATATGAGACTGAGAGCTGGATGGATGAAGAGACATCGTCCTGGATGGACAGGGATCTCACGGACAGCGATTTGCGGTACCGCTTGCTAATGCAGAACCTCGAGGAACTGGAGACCAGCTTGGTTGGTAAAGACCTGGAGATGGACATCCTTGAGCAGCTTGGGGCTCTGGGATCGTTCAACGCTTCTGTGGCCAGGGGTGTCTCGGCGTTACATGGTCGACCAGATGGCTCTCTGCTTGATGATCCGGAGCCGGAGACGACTCCTCTGGATGCGGGGCAAGAAGACAGCCAAGTGGTCGTCGTCCGGAGTGGCAAGAGCCAGGAGAGGAAGCTGAGAAGGATGAGAGCATCATCAGATGAGAAAGGATCCAGGACCTCGGTGAAGGTGAACCCACCACGAAGACCCAAGAAATCCCGTAAGGCGAGTAGCAGCCAGTTTATATCCGAGTGGAAAAGTCACCCAGGCCAAAGGAGGATCATTGTGCGTGAGCAATCAGCACTGCTGTCCACCATCAAGGAATGTGCAGGTCTTGAGAAGATAAGGGAGAAGATGGTGAAGGAAGGGCAGGAGGTGAGCTACCACAGGTGGGCGGAGGCAGCTGGGGTGGACGAGGCAGAGCTGGTGAGCAGGCTGCAGGCAGGTTACTGCTGCCGGGACAGGCTACTGGTGACCACCGAGTGGCTGGTCAGGTGCATCGCGAGAACATACACCGGCATGGGGACGGCCTTGGACGATCTGCTTCAGGCTGGGAAGATGGGCGTTCTTGACGGCGCCGAGAAGTTCGACAGCCGGAAGGGGTGCAGGTTCTCCACGTACGTCAAGTACTGGATCAGGAAAGGGATGCTCGCTCTGCTGGCTGAAAACTCAGGAGTCACCCTGCTGCCTGCAAGAATGGAGAGCATCATGCGCAAGGTGAAGGAGGCGCGGCGTGGGATCCGGTACAGGCAGGGCAGGAACCCGTCGGACTCAGAGGTTGCCGCCGCCGTGGGCGTGTCGGTAGCCAACGTGAGGCTGGCGCGCAAGTGCTCTCGCAGGGCCGTCTCGCTCTACTCGGAGGTCGCGATCGGACAGGACGTCAAGTTCGCGGAGGTGATCCCGGACGAGTCGGCGTCTCCGGACGAGGCGGCGCTGTTCCGGGGCCAGCTGAGGGAGCGGCTGCTGCTGGTGCTGGGCAGGCTGCCCGCGCGGGAAGGACGCGTGCTGCGGCTGCGGCACGGGCTGGAGGACGGGCGGTGCAGGTCGCTGGAGGAGATCGGCGGCATCTACGGCGTCTCCAAGGAGTGGATCAGGAAGATCGAGAAGTCGGCCATGGCCAGGCTCCGGGACGACGACGCCGTGCGCCGCGACCTCCACGACTTCGTC
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