| TFome pUT5629 | |
|---|---|
| Template | BT087988 |
| Genbank Insert ID | KJ727690 |
| Protein Name | ZmbHLH171 |
| Species | Zea mays |
| TF Family | bHLH |
| Gene id | GRMZM2G024530 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT5629
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | UT3296up |
| Temperature (ºC) | 63.7 |
| Sequence
|
caccATGGACTTCTCAGCTGGTTCCTACTTCA |
3' Primer
| Name | UT3296dn |
| Temperature (ºC) | |
| Sequence
|
CTGTTCGGGAGGGATTGACGC |
PCR Condition
Amino Acid Sequence
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MDFSAGSYFTSWPVNSASESYSLADGSVESYGGEGIMPPSSYFMTARSDHNLKFSVHEQDSTMLPNDQLTYAGARQTDLLPGETPSRDKLCENLLELQRLQNNSSLPSNLVPPGVLQHNSTPGAFHPQLNTPGLSELPHALSSSIDSNGSEVSAFLADLNAVSSASALCSTFQNASSFMEPVNLEAFSFQGAQSDSVLNKTTHPNGNISVFDSAALASLHDSKEFISGRLPSFASVQETNVAASGFKTQKQEQNAVCNVPIPTFTARNQIAVAAMPGSLIPQKIPSWINENKSEGPVSHPSDVQIQPNSVGNGVGVKPRVRARRGQATDPHSIAERLRREKISDRMKNLQDLVPNSNKADKASMLDEIIDHVKFLQLQVKVLSMSRLGAPGAVLPLLAESQTEGYRGQLLSAPTNAQGLLDTEESEDTFAFEEEVVKLMETSITSAMQYLQNKGLCLMPVALASAISTQKGVSAASIPPEQ
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGACTTCTCAGCTGGTTCCTACTTCACATCATGGCCTGTCAATTCTGCTTCTGAGAGCTACAGTTTGGCTGATGGTTCAGTTGAATCCTATGGAGGAGAAGGAATCATGCCACCTTCAAGCTACTTCATGACAGCCAGATCAGATCACAATTTAAAATTCAGTGTGCATGAACAGGATTCCACTATGCTTCCAAACGACCAATTGACCTATGCTGGCGCTAGGCAGACTGATCTATTACCTGGCGAGACTCCATCTAGGGATAAGCTTTGTGAGAATCTTCTGGAGCTTCAACGACTGCAGAATAACAGCAGCCTACCGAGTAATTTAGTGCCCCCAGGGGTACTTCAGCACAATTCAACACCTGGGGCCTTTCATCCGCAGTTGAATACTCCTGGACTTTCAGAACTGCCTCATGCCTTATCTAGTTCAATTGATAGTAATGGAAGTGAAGTTTCTGCTTTTCTCGCTGATCTAAATGCTGTTTCTTCAGCCTCAGCTTTGTGTTCCACATTCCAAAATGCTTCTTCATTCATGGAACCAGTAAATCTAGAAGCTTTCAGTTTTCAAGGGGCACAAAGTGATTCTGTTTTGAACAAAACAACACATCCAAATGGGAATATCTCAGTATTTGACAGTGCTGCCTTGGCATCACTACATGATAGCAAAGAGTTTATCAGTGGTAGGCTCCCCTCATTTGCTAGTGTCCAGGAAACAAACGTAGCTGCTAGTGGTTTCAAGACTCAGAAGCAGGAGCAAAATGCAGTGTGCAACGTTCCTATCCCTACGTTCACTGCACGTAATCAAATTGCAGTTGCTGCAATGCCCGGATCACTGATCCCTCAAAAGATTCCTTCATGGATCAATGAAAACAAAAGTGAGGGTCCTGTTAGCCATCCTTCTGATGTACAAATCCAACCAAATTCTGTTGGAAATGGTGTTGGTGTGAAGCCACGAGTAAGGGCTCGTCGTGGACAGGCAACTGATCCTCATAGCATTGCTGAACGACTTCGCAGAGAAAAAATTTCAGATAGGATGAAAAATCTACAAGATCTTGTCCCGAATTCTAATAAGGCAGACAAGGCATCCATGCTGGATGAAATCATTGATCATGTGAAATTTCTTCAGCTTCAGGTCAAGGTGTTAAGCATGAGTAGGCTAGGAGCTCCAGGAGCAGTTCTTCCCCTTCTCGCAGAATCTCAGACTGAGGGTTACCGCGGTCAACTTCTGTCAGCTCCAACCAACGCACAAGGATTACTGGACACGGAAGAATCGGAAGACACCTTCGCCTTCGAGGAAGAGGTTGTGAAGCTAATGGAAACCAGCATCACAAGCGCAATGCAGTACCTTCAGAACAAGGGACTCTGCCTGATGCCAGTTGCCCTTGCTTCCGCCATATCCACCCAGAAGGGCGTCTCCGCCGCGTCAATCCCTCCCGAACAG
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