| TFome pUT5657 | |
|---|---|
| Template | BT054170 |
| Genbank Insert ID | KJ727718 |
| Protein Name | ZmMTERF15 |
| Species | Zea mays |
| TF Family | mTERF |
| Gene id | GRMZM2G119921 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.T565C p. L189(=) SNP not present in template FLcDNA |
| Request Information | Deposited to ABRC |
TFome clone pUT5657
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B842F |
| Temperature (ºC) | 66.0 |
| Sequence
|
caccATGAACAACCTCCGGAAGCGCATGATCTC |
3' Primer
| Name | B842R |
| Temperature (ºC) | |
| Sequence
|
GCTTTCTTTATTTTCGCCTGTCATCTTATATAGCCGTTCCC |
PCR Condition
ddH2O- 9.75 GC buffer-5.0 50% glycerol-5.0 dNTPs (
Amino Acid Sequence
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MNNLRKRMISLLLRAPGSPYVPCPHFFPLFYPHRLLSATKCVATKPFAVEDYLVATCGLTQAQALKASKHVSHLKDPSKADAVVAFLSNLGLSSAEITDVVTRDPKVLCSDVERSLTARVAELTDLGFSRPEIVRLLIVGMNHFRHSSLRLNLEFWISVFGSLDELIRALRINAALLSTRIEEVCKPNLELLQECGINVSDISNSFMSRVLTRDPKSLQEALARLHEFRIQPGSQPFFRGLYTFAILGSGKITKSIQLFEKLGWSKDHIVSAVKRDPTILGFTEERVRRNMEFLIRVVGLEVPYIARRPALINYSIDRRLLPRNCLINFLRAKGLFNDEASFLSVAAIGDEKFRRRYVHPYEEDFPGLAAAFASSCAGEHQWERLYKMTGENKES
TFome clones do not contain stop codon at 3'end.
CDS
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ATGAACAACCTCCGGAAGCGCATGATCTCCCTTCTCCTCCGGGCACCTGGATCACCATACGTCCCTTGCCCCCACTTCTTCCCGCTCTTCTATCCCCACCGCCTCCTCTCCGCCACCAAATGCGTCGCTACAAAACCATTCGCCGTCGAGGACTACCTCGTCGCCACATGCGGCCTCACCCAAGCACAGGCCCTCAAGGCGTCGAAGCATGTCTCCCATCTGAAGGATCCCTCGAAAGCCGACGCGGTCGTCGCCTTCCTCTCCAACCTCGGCCTGTCCAGCGCCGAGATCACCGACGTCGTGACCAGGGACCCCAAAGTCCTCTGCTCCGACGTGGAGAGATCCCTCACCGCTCGCGTCGCCGAGCTCACCGACCTCGGGTTTTCACGTCCGGAGATCGTCCGCCTTCTCATCGTTGGCATGAACCACTTCCGCCATAGCTCGCTTCGCCTCAATCTTGAGTTTTGGATCTCGGTCTTTGGCTCTCTCGACGAGCTCATTCGGGCGCTCAGAATTAACGCCGCTCTCCTCAGCACAAGGATTGAGGAGGTCTGCAAGCCCAACCTGGAGCTCCTTCAAGAGTGTGGTATAAATGTTTCTGATATCTCCAACAGCTTCATGTCCCGTGTGCTCACCAGGGACCCTAAGAGCCTGCAGGAAGCTTTGGCTCGCCTTCATGAGTTCAGAATCCAGCCGGGCTCTCAACCTTTCTTCCGTGGGCTTTATACATTTGCAATCCTTGGCAGCGGGAAGATAACCAAGAGCATTCAGCTGTTTGAGAAGCTTGGATGGTCAAAGGACCATATAGTATCAGCGGTGAAGAGAGACCCGACTATATTGGGTTTCACCGAGGAGAGGGTTCGAAGAAACATGGAATTCTTGATCAGGGTTGTCGGATTGGAGGTACCATACATCGCACGAAGGCCAGCGCTGATAAATTATAGTATTGACCGTCGGTTGTTGCCACGGAATTGCTTGATCAATTTTCTCAGGGCAAAGGGGTTGTTCAATGACGAGGCTAGTTTCCTTTCTGTCGCCGCTATAGGCGATGAGAAATTTCGGCGCCGATACGTGCATCCTTACGAGGAGGATTTTCCAGGCCTTGCTGCTGCTTTTGCTTCTAGCTGTGCTGGAGAACACCAATGGGAACGGCTATATAAGATGACAGGCGAAAATAAAGAAAGC
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