| TFome pUT5660 | |
|---|---|
| Template | BT068699 |
| Genbank Insert ID | KJ727720 |
| Protein Name | ZmLBD20 |
| Species | Zea mays |
| TF Family | LBD |
| Gene id | GRMZM2G092517 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.C174T p.(=). Not in template FlcDNA. |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT5660
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B891F |
| Temperature (ºC) | 62.6 |
| Sequence
|
caccATGTCCACGGAGAGGGAGAG |
3' Primer
| Name | B891R |
| Temperature (ºC) | |
| Sequence
|
ACCAAAGTATGCAGGATGCTCACTTG |
PCR Condition
Step 1, 98°C 40 sec melt, 1 cycle; Step 2, 98&
Amino Acid Sequence
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MSTERERLDEIGKIKREPDTAALAVAAASASTVPADNHIPRRLGHGGALNTLTPCAACKLLRRRCAQECPFAPYFSPHEPHKFAAVHKVFGASNVSKMLLEVVEGERADTASSLVYEANLRLRDPVYGCMGAISILQQQVNALEAELEAVRAEILKHRYRQATGNRLMDDVRATASFVLAPASAPMHVNDVVSVVEAGQEVTATGGASGVYIAEAEQPSSTNNYSSLNPSEHPAYFG
TFome clones do not contain stop codon at 3'end.
CDS
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ATGTCCACGGAGAGGGAGAGACTCGATGAGATCGGGAAGATCAAGCGAGAGCCGGATACAGCCGCCCTCGCTGTCGCTGCCGCATCCGCCTCCACCGTGCCGGCCGACAACCACATCCCACGCCGCCTCGGCCACGGCGGTGCCCTCAACACCTTGACGCCGTGCGCCGCGTGTAAGCTCCTCCGCCGCCGCTGCGCGCAGGAATGCCCCTTCGCCCCTTACTTCTCGCCGCATGAGCCCCACAAATTCGCCGCCGTCCACAAGGTCTTCGGCGCTAGCAACGTCTCCAAGATGCTCTTGGAGGTGGTGGAGGGGGAGAGGGCGGACACAGCGAGCAGCCTGGTGTACGAAGCGAACCTCCGGTTGCGGGATCCGGTGTACGGATGCATGGGCGCCATCTCCATTCTCCAGCAACAGGTGAACGCGCTGGAGGCCGAGCTGGAGGCAGTGAGGGCCGAGATCCTCAAGCACAGGTACCGGCAGGCCACCGGCAACCGTCTCATGGACGACGTGCGTGCCACGGCCAGCTTCGTCTTGGCTCCTGCGTCCGCTCCGATGCACGTTAATGACGTTGTATCAGTGGTGGAGGCAGGCCAAGAGGTCACCGCCACCGGCGGCGCGTCGGGGGTGTACATTGCGGAAGCCGAGCAGCCGTCAAGTACTAATAACTATAGCTCCCTTAACCCAAGTGAGCATCCTGCATACTTTGGT
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