| TFome pUT5673 | |
|---|---|
| Template | BT069784.1 |
| Genbank Insert ID | KJ727733 |
| Protein Name | ZmbZIP73 |
| Species | Zea mays |
| TF Family | bZIP |
| Gene id | GRMZM2G175870 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.A152G p.H51R Not present in template FlcDNA. Not in functional domain. |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT5673
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B531_F |
| Temperature (ºC) | 62.9 |
| Sequence
|
caccATGGACGATGGAGCTGACCTC |
3' Primer
| Name | B531_R |
| Temperature (ºC) | |
| Sequence
|
TTCAGCCAGGGGATCAGAACTCAC |
PCR Condition
PCR Reaction Mix:5 l GC buffer (NEB) 1 l primer fo
Amino Acid Sequence
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MDDGADLPCQLLFSHPEMPDSFDEFFNSATTTCTHTHSCNPPGPSVAMHTRTCLHAHTLVLGSGGEDDDDAREDSAKPRRPLGNREAVRKYREKKKAHAAFLEEEVKKLRAANQQLQRRLQGHAALEAEVARLRGLLLDIRGKIDAEVGGVLPFQKPCSVGSVACADPALCFNGNSEVGGGWEESSRPAAADCRIDEVRGMSREIDVPEGLRHSMDVVASFVSSDPLAE
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGACGATGGAGCTGACCTCCCCTGCCAACTGCTCTTCTCCCACCCGGAGATGCCTGACAGCTTCGACGAGTTCTTCAACAGCGCCACGACGACCTGCACCCACACGCACAGCTGCAACCCGCCCGGCCCCTCGGTCGCCATGCACACCCGCACGTGCCTGCACGCGCACACCCTGGTCCTGGGCAGCGGCGGGGAGGACGACGACGACGCCAGGGAGGACTCGGCGAAGCCGCGCAGACCGCTGGGGAACAGGGAAGCTGTGCGCAAGTACCGGGAGAAGAAGAAGGCGCACGCGGCCTTTCTGGAAGAGGAGGTCAAGAAGCTCCGCGCCGCCAACCAGCAGCTCCAGAGACGGCTGCAGGGCCATGCAGCTTTGGAGGCGGAGGTGGCGAGGCTCCGGGGTCTCCTGCTCGATATCCGGGGCAAGATCGACGCCGAGGTCGGCGGCGTGCTCCCTTTTCAGAAGCCGTGCAGTGTGGGCTCCGTGGCGTGTGCCGATCCTGCTCTGTGCTTCAACGGCAACTCGGAGGTTGGAGGCGGCTGGGAGGAGAGCTCAAGACCGGCAGCCGCGGACTGCAGGATCGATGAAGTGCGAGGGATGTCACGGGAAATTGATGTCCCAGAAGGCTTGCGTCATTCCATGGATGTTGTTGCGAGCTTCGTGAGTTCTGATCCCCTGGCTGAA
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