| TFome pUT5676 | |
|---|---|
| Template | BT083532.1 |
| Genbank Insert ID | KJ727735 |
| Protein Name | ZmEREB13 |
| Species | Zea mays |
| TF Family | AP2/ERF-AP2 |
| Gene id | GRMZM2G011110 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.C45A p.(=) c.C118A p.Q40K Present in functional domain c.C216A p.(=) None present in template FlcDNA |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT5676
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B367_F |
| Temperature (ºC) | 73.3 |
| Sequence
|
caccATGGTGAAGGGCGCCCAGCTC |
3' Primer
| Name | B367_R |
| Temperature (ºC) | |
| Sequence
|
GTTGAGGGACGAGAAGCTCCAGAGGTCG |
PCR Condition
PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer f
Amino Acid Sequence
Copy to clipboard
MVKGAQLQDAAADAARNAAAAAARQQGCGGGGPAGCGKRKYKGVRMRSWGSWVSEIRAPNQKTRIWLGSYSTAEAAARAYDAALLCLKGSAADLNFPVHLPFHIPAAAMSPKSIQRVAAAAAANATCGSPLQAGAGAAAAAPHYYSYSSSAASTMAADGATPPCSYGDASSGVSSPETAGNTDDLCYGVAHDDDVDMVAGDADLAALADIESFFRSPKCMEYAMMDPCSTFFAPAPMATDAASEWEWEEEGEIDLWSFSSLN
TFome clones do not contain stop codon at 3'end.
CDS
Copy to clipboard
ATGGTGAAGGGCGCCCAGCTCCAGGACGCGGCGGCCGACGCCGCACGCAATGCGGCGGCGGCGGCGGCGAGGCAGCAGGGCTGTGGTGGCGGCGGCCCGGCGGGCTGCGGCAAGAGGAAGTACAAGGGCGTGCGGATGCGGAGCTGGGGCTCCTGGGTGTCGGAGATCAGGGCGCCCAACCAGAAGACCCGGATATGGCTCGGCTCCTACTCCACAGCCGAGGCCGCCGCGCGCGCCTACGACGCCGCCCTGCTCTGCCTCAAGGGCTCCGCCGCCGACCTCAACTTCCCCGTGCACCTCCCCTTCCACATCCCCGCCGCCGCCATGTCGCCCAAGTCCATCCAGCGCGTCGCCGCCGCCGCCGCGGCCAATGCCACCTGCGGCAGCCCGCTGCAGGCCGGCGCCGGCGCCGCCGCCGCCGCGCCCCACTACTACTCCTACTCCTCCTCCGCCGCCTCCACGATGGCCGCCGACGGCGCCACCCCTCCGTGCAGCTACGGCGACGCGTCGTCGGGCGTGAGCTCCCCGGAGACGGCCGGGAACACTGATGACCTGTGCTACGGCGTGGCGCACGACGACGACGTGGACATGGTGGCGGGCGACGCGGACCTCGCCGCGCTCGCGGACATCGAGTCCTTCTTCCGGTCGCCCAAGTGCATGGAGTACGCTATGATGGACCCATGCAGCACGTTCTTCGCGCCAGCGCCCATGGCGACGGACGCGGCCAGCGAGTGGGAGTGGGAGGAGGAAGGCGAGATCGACCTCTGGAGCTTCTCGTCCCTCAAC
Copyright © 2023 Grassius.org | Last updated: 2023-06-26