TFome clone pUT5681

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	R1-F
Temperature (ºC)	68.2
Sequence Copy to clipboard	caccATGAAGCTCTCGCCGTCGGCCA

3' Primer

Name	R1-R
Temperature (ºC)
Sequence Copy to clipboard	GAACTCGACCGAACCCACCGGC

PCR Condition

Amino Acid Sequence

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MKLSPSASGGLQDQPASPEEAEEHKCLNSELWHACAGPLVSLPSVGSRVVYFPQGHGEQVAASTNKEMEAQIPNYPSLPPQLICQLHNVTMHADAETDEVYAQMTLQPLSPQELKDPFLPAELGTASNQPTNYFCKTLTASDTSTHGGFSVPRRAAEKVFPPLDFNQQPPAQELIAKDLHGNDWKFRHIFRGQPKRHLLTTGWSVFVSAKRLVAGDSVLFIWNDNNQLLLGIRRANRPQTVMPSSVLSSDSMHIGLLAAAAHAASTNSRFTIFYNPRASPCEFVIPMAKYVKAVYHTRISVGMRFRMLFETEESSVRRYMGTITGISDLDPVRWPNSHWRSVKVGWDESTAGEKQPRVSLWEIEPLTTFPMYPSPFALGLKRPWPAGLPSLYGGRGDGLTSSLMWLRDRANPGFQSLNFSGLGMSPWMQPRLDNSLLGLQSDMYQTIAAAAALQSTTKQVPPSAMQFQQPQNIADRSALLSSQILQQVQPRFQQIYPQNLNENKIQGHTQPEYLQVQQQLQRCQSFNEQKPPMHPQQQQQEPQQQQCVQTPQDQQMQEQKHLHNFHSLPDALSAFSQLSPATHSPPSALQTVPAFSHQQNFPDTNISSLSPSTGPSMHGMLGRLPSEAASSLPCVAMNAPVSVSDPWSSKRVAVESVNPCRPHVSPHIEHLDMATCNMPQSSALAPLPGRECLVDEDGCSDPQNHLLFGVHIDSHSLLMQGGIPALQNDNSSGTIPYSTSNFLSPSENDFPLNQPLRSAGCLDESDYLPCAENAEQANQQFATFVKVYKSGTVGRLLDITRFSSYDELRSEVGRLFGLEGQLEDPLRSGWQLVFVDREDDVLLVGDDPWQEFVNSVSCIKILSPEEVQRMGKPGIQLLSSAPSRRLGNGCDSYASMQEPRGLDAGMAPVGSVEF

TFome clones do not contain stop codon at 3'end.

CDS

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ATGAAGCTCTCGCCGTCGGCCAGCGGTGGCCTCCAGGACCAGCCGGCGTCGCCGGAAGAAGCTGAAGAGCACAAGTGCCTGAATTCTGAGTTGTGGCATGCATGTGCTGGCCCTCTAGTTTCATTGCCTTCGGTCGGTAGTCGAGTGGTGTATTTTCCTCAAGGCCACGGTGAGCAGGTAGCAGCATCAACAAATAAGGAAATGGAGGCTCAGATCCCCAACTATCCTAGTCTACCTCCGCAGCTTATATGCCAACTGCATAATGTGACTATGCATGCTGATGCTGAGACAGATGAGGTGTATGCACAGATGACACTCCAGCCACTCAGTCCACAAGAGCTCAAGGACCCATTTTTGCCTGCTGAGTTAGGCACTGCCAGCAACCAGCCAACAAATTATTTTTGTAAAACACTAACTGCAAGTGATACGAGTACACATGGTGGGTTCTCTGTTCCACGCCGAGCAGCTGAGAAGGTGTTTCCTCCACTGGATTTCAATCAGCAACCTCCCGCACAGGAGTTGATTGCAAAAGATCTTCATGGCAACGACTGGAAATTTCGCCATATTTTTCGAGGTCAGCCAAAGCGGCATCTTCTGACAACAGGTTGGAGTGTCTTTGTTAGTGCAAAGAGACTGGTTGCTGGCGACTCTGTCCTTTTTATCTGGAATGACAACAACCAGCTGCTTCTGGGAATTCGCCGGGCAAACAGACCACAAACCGTCATGCCATCATCAGTACTTTCTAGTGATAGCATGCATATTGGCCTGCTCGCTGCTGCAGCTCATGCTGCATCAACAAATAGCCGGTTTACTATCTTCTATAATCCAAGAGCAAGCCCTTGTGAATTTGTCATACCCATGGCTAAGTATGTGAAAGCTGTGTACCATACCCGTATATCTGTGGGCATGCGTTTCCGGATGCTTTTTGAGACGGAGGAATCTAGTGTAAGGCGTTATATGGGGACAATTACAGGAATAAGTGATCTTGATCCTGTTCGCTGGCCAAACTCGCACTGGCGCTCAGTAAAGGTTGGATGGGACGAGTCAACTGCTGGAGAGAAGCAGCCAAGGGTATCTCTTTGGGAGATTGAACCGTTGACAACTTTTCCAATGTATCCATCTCCTTTTGCTCTTGGATTAAAGCGCCCATGGCCTGCAGGCCTGCCTTCTTTATACGGTGGAAGGGGTGATGGCTTGACCTCTTCTCTTATGTGGCTTCGAGACAGGGCAAACCCGGGTTTCCAGTCACTGAATTTCAGCGGGCTTGGTATGAGTCCCTGGATGCAGCCTAGGCTGGATAATTCCTTACTTGGTCTCCAATCTGACATGTACCAGACCATAGCAGCAGCAGCAGCATTACAAAGCACTACGAAGCAAGTTCCACCTTCAGCAATGCAGTTCCAACAGCCTCAGAACATTGCTGATAGATCTGCTCTTCTATCAAGTCAGATTCTGCAGCAAGTACAACCTCGGTTTCAGCAAATTTACCCTCAAAATCTCAACGAAAACAAAATTCAAGGTCATACACAACCTGAGTACCTCCAAGTCCAACAGCAGCTTCAACGCTGCCAATCATTTAATGAGCAGAAGCCTCCGATGCATCCACAGCAACAACAGCAGGAACCACAGCAGCAGCAATGCGTCCAAACACCACAGGATCAACAAATGCAAGAACAGAAGCATCTGCACAACTTCCACTCCTTGCCAGATGCATTATCGGCCTTTTCTCAGCTTTCCCCAGCCACTCACTCCCCACCTTCCGCATTGCAGACAGTTCCAGCATTCTCGCATCAACAAAACTTCCCAGATACAAACATTAGCTCTCTCTCACCATCTACTGGCCCTTCAATGCATGGCATGTTGGGGAGATTACCTTCCGAAGCAGCTTCGAGCCTCCCATGTGTGGCAATGAACGCCCCTGTATCGGTATCTGACCCATGGTCATCCAAGAGAGTTGCAGTGGAGTCTGTTAACCCTTGTCGACCTCATGTTTCACCACATATAGAACATTTGGATATGGCGACGTGTAACATGCCGCAGAGCTCTGCACTAGCACCATTACCTGGACGGGAATGCCTAGTGGATGAAGATGGGTGCTCTGATCCTCAAAATCACCTCTTGTTTGGTGTTCACATAGACTCGCATTCACTTCTGATGCAAGGGGGCATTCCAGCCCTTCAGAATGATAACAGTTCAGGCACTATTCCATACTCAACCTCCAATTTCCTGAGCCCTTCTGAGAACGATTTCCCCTTGAATCAACCGCTACGCAGCGCGGGCTGCTTAGATGAGTCGGATTATCTTCCATGTGCAGAGAATGCCGAACAGGCAAATCAACAGTTTGCAACCTTTGTGAAGGTTTACAAATCCGGGACCGTCGGAAGGTTGCTCGACATCACTAGATTCAGCAGCTACGACGAGCTCCGTAGCGAGGTGGGGCGTCTGTTTGGCCTCGAGGGCCAGCTGGAAGACCCTCTGAGATCAGGCTGGCAGCTTGTATTTGTTGACAGGGAGGACGATGTCCTTCTGGTTGGCGATGACCCATGGCAGGAATTCGTGAACAGTGTGTCCTGTATAAAGATACTTTCACCGGAGGAGGTCCAGCGGATGGGAAAGCCGGGCATCCAGCTCCTGAGCTCGGCTCCTTCAAGAAGGCTGGGCAATGGCTGCGACAGCTACGCGAGCATGCAGGAGCCGAGAGGCCTGGACGCTGGGATGGCGCCGGTGGGTTCGGTCGAGTTC