| TFome pUT5699 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ727756 |
| Protein Name | ZmNLP3 |
| Species | Zea mays |
| TF Family | NLP |
| Gene id | GRMZM2G031398 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.Start +492 1366ins18. Support in RNA-seq. Not present in functional domain. |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT5699
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | R81F |
| Temperature (ºC) | 66.2 |
| Sequence
|
caccATGGATGTGCCTACGCCTCCCAAC |
3' Primer
| Name | R81R |
| Temperature (ºC) | |
| Sequence
|
GGCGCTTCCTTCCACCTGCAAGC |
PCR Condition
Amino Acid Sequence
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MDVPTPPNRAGCNSNISNIGSPMQSFDDPFGGAAMTSFDGYSELSSPSVADHIFSLLNDPSSVQHMFALWSSSGSSPRASAVREDMSFDTYPGPMDVTASLAQRINSASVFNPTEVDRGLKDSDGLVPNNGSQQGSSIIPRSVGNILADRMLMALSLFRKSLSDGVLAQVWMPVEHNGRVVLSTSEQPYLLDQDLAGYREVSRNFLFSVKEEPGLHLGLPGRVFISGVPEWTSSVTYYSKPEYLRMEHALLHEIRGSLAMPVYDPSKGSCCAVLELVTNKEKPDFDAEMDIVCDALQGVNLQTTTDRSNQKVYSENQKYASTEILDVLRAICHAHMLPLALTWIPTSNGTANGFCVAKNIRLDSQPGKSVLRIHESACYVNDAKMQGFLHACAERHLEKGQGIAGRALKSNLPFFSPDIREYSIEDYPLAHHARKFGLHAAVAIRLRSTYTGKDDYILEFFLPVNCKGCGEQQMLLNSLSSTMQRICKSLRTVSEAETDSVSATEPMYEKTNGSCLPTGNSESSSHDDQPITESAFHDLSSGDKQGDREPAKARSSSKRVAEKKRSTSEKNFSLDVLRKYFSGSLRDAAMSLGVCPTTLKRICRQHGISRWPSRKINKVNRSLKKIEKVIKSVHGVDRSLQYDPATGSLVPATSLPDKMPTSSAGVKTVEEKSSPEPGQDFSSPDGWQRESSQLHASSIPTRVGDEVQMLAINNEGSRNYASGIANIAQHSNSEDAHGPLYPVGAVNSSRTGETGYIDSPTSLHMDSVEGQTTVRDSRSVQQADVTMATKEQTLPSTSGTTDSSSGSASSQPAFKGNPGRALKVSSSDALTVKATCNGDTVRFKFLPAMGWYHLLEEVAKRFKLATGAFQLKYKDDEDEWVILANDADLQECVDVLDSIGSRSVKLQVRDLPCLVSSSGSSSCCLQVEGSA
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGATGTGCCTACGCCTCCCAACCGTGCCGGCTGCAACAGTAACATCAGCAACATCGGCAGCCCCATGCAATCTTTTGATGATCCCTTTGGTGGTGCTGCCATGACGAGCTTTGATGGATACTCGGAGCTCAGCAGCCCATCAGTTGCTGATCACATATTCTCCCTGCTGAATGATCCCTCGTCTGTGCAACATATGTTTGCCTTGTGGTCATCTTCGGGGTCATCTCCGCGTGCTTCTGCTGTGAGAGAAGACATGTCGTTTGACACCTATCCTGGACCTATGGACGTGACAGCATCACTTGCTCAAAGGATCAACTCAGCATCAGTATTCAATCCAACTGAAGTGGACAGAGGACTAAAAGACTCAGATGGGCTTGTTCCAAATAATGGTTCACAACAAGGGAGTAGCATCATTCCGAGGTCGGTTGGCAACATTCTTGCAGACAGGATGCTCATGGCGCTATCTTTGTTCAGGAAGTCATTGAGCGATGGTGTTCTTGCCCAAGTCTGGATGCCTGTTGAGCACAATGGTCGTGTCGTGTTGAGTACATCCGAGCAGCCCTATTTACTTGACCAGGATCTTGCAGGTTACAGAGAAGTTTCTAGAAATTTCTTGTTTTCAGTTAAGGAGGAGCCTGGTCTGCACCTAGGGCTTCCAGGGAGGGTCTTCATATCTGGGGTGCCAGAGTGGACCTCAAGTGTTACGTATTACAGTAAGCCAGAGTATTTGAGAATGGAGCATGCTCTTCTTCATGAAATACGAGGGTCACTTGCTATGCCAGTTTATGATCCCAGCAAGGGTTCTTGTTGTGCAGTTCTTGAACTTGTCACAAACAAGGAGAAACCTGATTTTGATGCAGAGATGGACATTGTTTGTGATGCCTTGCAGGGTGTGAACTTGCAGACAACAACAGATCGCAGCAATCAGAAGGTTTACTCTGAAAATCAGAAATATGCTTCTACAGAGATTTTGGATGTTCTAAGAGCTATTTGCCATGCACATATGCTTCCTTTGGCCCTTACATGGATCCCTACATCAAACGGCACCGCTAATGGTTTTTGTGTCGCAAAGAATATCAGGCTTGATTCGCAACCAGGAAAATCAGTACTTCGTATCCATGAATCAGCTTGTTATGTTAACGATGCAAAGATGCAGGGATTTCTTCATGCATGTGCTGAACGTCATCTTGAGAAAGGGCAGGGTATTGCAGGCCGAGCCCTTAAATCTAATCTTCCATTCTTTTCGCCTGATATAAGAGAGTACAGTATCGAAGACTACCCGCTTGCACACCATGCTCGTAAGTTTGGTCTGCATGCTGCTGTAGCCATCCGACTGAGGAGTACGTACACTGGTAAGGATGACTACATACTAGAATTCTTTCTCCCAGTCAACTGCAAGGGCTGTGGAGAGCAGCAGATGTTATTAAACAGCCTTTCCAGCACAATGCAAAGAATATGCAAAAGCTTGCGAACAGTTTCTGAAGCAGAGACCGATAGTGTTAGTGCTACTGAACCAATGTATGAGAAGACTAATGGAAGTTGTTTACCAACTGGTAACTCTGAAAGTTCCTCACATGATGACCAGCCAATCACAGAATCTGCATTCCATGATCTATCTTCGGGTGACAAACAAGGAGACAGAGAACCTGCTAAGGCACGGAGTAGCTCAAAGCGAGTTGCAGAGAAAAAACGCAGTACATCTGAAAAGAATTTTAGTCTGGATGTTCTTCGCAAATACTTTTCTGGGAGCCTCAGGGATGCTGCAATGAGCCTTGGCGTTTGTCCAACAACTTTGAAACGAATATGCAGACAACATGGAATTTCCAGATGGCCATCTCGAAAGATAAACAAGGTCAACCGTTCACTGAAGAAGATCGAGAAGGTCATCAAATCGGTTCATGGTGTGGACAGATCCTTGCAGTACGATCCTGCCACTGGATCTCTTGTTCCAGCAACCTCCCTTCCGGATAAGATGCCTACCTCATCTGCGGGCGTAAAAACAGTGGAGGAAAAATCTAGCCCAGAACCAGGACAGGATTTCTCTTCGCCTGATGGATGGCAAAGAGAAAGTAGCCAACTTCATGCCTCCAGTATACCTACAAGGGTAGGTGATGAAGTCCAAATGCTGGCAATCAACAACGAAGGCAGCAGGAACTATGCTTCTGGTATCGCAAATATCGCGCAGCATTCAAACTCCGAAGACGCACACGGTCCTTTATATCCCGTCGGTGCTGTTAATTCTTCGCGTACCGGAGAAACAGGCTACATCGATTCTCCAACCTCCCTCCACATGGATAGCGTCGAAGGCCAAACAACGGTGAGGGACTCAAGATCTGTACAGCAAGCAGATGTCACCATGGCCACCAAGGAGCAGACCCTTCCCTCCACCTCTGGCACAACCGATTCTTCGAGCGGCAGCGCCTCAAGTCAGCCTGCTTTCAAGGGGAACCCAGGACGTGCCCTCAAAGTTAGCAGCAGCGATGCACTGACGGTGAAAGCTACCTGTAACGGCGACACCGTGCGGTTCAAGTTCCTGCCGGCAATGGGCTGGTACCACCTGCTGGAAGAGGTAGCGAAGAGGTTTAAGCTGGCGACAGGAGCGTTCCAGCTCAAATACAAGGACGACGAGGACGAGTGGGTGATCCTGGCGAACGACGCCGACCTCCAGGAGTGCGTGGACGTCCTGGACTCGATCGGCTCGCGCAGCGTGAAGCTGCAGGTGCGCGACCTCCCCTGCCTCGTCAGCAGCTCGGGCAGCAGCAGCTGCTGCTTGCAGGTGGAAGGAAGCGCC
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