| TFome pUT5711 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ727765 |
| Protein Name | ZmDOF23 |
| Species | Zea mays |
| TF Family | C2C2-Dof |
| Gene id | GRMZM2G010290 |
| transcript | T02 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.T801A p.267(=) c.C802T p.L268(=) |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT5711
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | R177F |
| Temperature (ºC) | 66.2 |
| Sequence
|
caccATGGATGCAGCAGCCCACTGG |
3' Primer
| Name | R177R |
| Temperature (ºC) | |
| Sequence
|
CCAAGAAGCGCCACTGCCG |
PCR Condition
Amino Acid Sequence
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MDAAAHWQQSLGLVKPLEEMLMAASAGAANPSQGSNPNPPPLAAPLTDAGSTERRARPQKEKALNCPRCNSTNTKFCYYNNYSLQQPRYFCKTCRRYWTEGGSLRSVPVGGGSRKNKRSSSSSSSSSSSAAAASASTSSSATSSSMASTPGAASKSPELAHEGAHDDLNLAFPHHGGLHAAEFAAFPSLESSNDMCDQGGGMTSNGRGGGAGPAVGALSATELLRSSGCYMPLQMPGGDYTAAGFALGEFRTPLLPPPSSQSPLGFSLDAHGPGSSGAAAAAGYGSSAGLPQGVPGNAGRLLFPFEDLKPPVSPGGGGVGGGGASATGGAGDGNSSHDQFDHNKERGGGGGRGAGAGHDTPGFWSSMIGGSGASW
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGATGCAGCAGCCCACTGGCAGCAGAGCCTAGGGCTGGTGAAGCCCCTGGAGGAGATGCTGATGGCGGCCAGCGCGGGGGCTGCAAATCCGAGCCAAGGCTCGAATCCGAACCCGCCGCCGCTGGCGGCGCCCCTGACGGACGCGGGTAGCACCGAGCGGCGCGCGCGGCCGCAGAAGGAGAAGGCGCTCAACTGCCCGCGGTGCAACTCCACCAACACCAAATTCTGCTACTACAACAACTACAGCCTCCAGCAGCCACGCTACTTCTGCAAGACGTGCCGCCGCTACTGGACGGAGGGCGGATCCCTCCGCAGCGTCCCCGTGGGCGGTGGCTCCCGCAAGAACAAGCGCTCCTCGTCCTCGTCCTCGTCCTCGTCCTCGTCGGCGGCGGCGGCGTCCGCCTCCACCTCCTCCTCGGCCACGAGCTCGTCCATGGCCAGCACACCGGGGGCGGCGTCCAAGAGTCCGGAGCTGGCGCACGAGGGCGCGCACGACGACCTCAACCTAGCGTTCCCGCACCACGGTGGCCTGCACGCCGCCGAGTTCGCGGCGTTCCCGAGCCTGGAGAGCAGCAACGACATGTGCGACCAGGGTGGCGGGATGACGAGCAATGGCCGGGGCGGCGGCGCGGGGCCCGCGGTCGGCGCGCTCTCGGCAACGGAGCTGTTGCGGAGCTCCGGCTGCTACATGCCGCTGCAGATGCCAGGAGGAGATTACACGGCGGCAGGGTTCGCGCTCGGGGAGTTCCGCACGCCGCTGCTGCCGCCACCATCGTCACAGAGCCCGCTCGGGTTCTCATTGGACGCGCACGGGCCGGGGTCGTCCGGTGCTGCCGCAGCGGCGGGGTACGGTTCCAGCGCGGGGCTGCCGCAGGGCGTGCCGGGCAACGCGGGCAGGTTGTTGTTCCCTTTCGAGGACTTGAAGCCGCCGGTTAGCCCTGGAGGTGGTGGTGTGGGCGGAGGTGGTGCAAGTGCAACCGGTGGCGCCGGCGACGGAAATAGCAGCCACGATCAGTTTGACCACAACAAGGAGCGAGGCGGAGGCGGCGGCCGCGGTGCCGGTGCCGGGCACGACACGCCGGGGTTCTGGAGCAGCATGATCGGCGGCAGTGGCGCTTCTTGG
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