| TFome pUT5769 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ727817 |
| Protein Name | ZmEREB192 |
| Species | Zea mays |
| TF Family | AP2/ERF-ERF |
| Gene id | GRMZM2G129777 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT5769
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | cd0038F |
| Temperature (ºC) | 61.4 |
| Sequence
|
caccATGTCTCCGTTGAACCAGCAA |
3' Primer
| Name | cd0038R |
| Temperature (ºC) | |
| Sequence
|
CACGCAGAAATCAACGGTTTG |
PCR Condition
Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98&
Amino Acid Sequence
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MSPLNQQQVLLKKALAKKPNAKKMSEFGLKPSAALLKSRTQQLPAPVQPRRRVRVLFEDPDATDSDSDSDDEEAGGTPSRTKRFSFEAFIGKAPAKPVLPAAAAVAACTSGGAPERYRGVRLRKWGKWAAEIRNPFTGKRQWLGTFDTAGAASAAYLSASRSFADEKRRRRGLPVAASSADTPASGSTPTASSSSSASTAPFAHPSPSSVLEPSKEARNTQSPEATPARPSTDSAQLPEDPEFYQDLLRGLQLPDIDPLDFRAGLDALDLSDADFGLDDEHDLLLGDFGDEEMEIDLDLDDINDVFPEMPGCDLGRGMDDFLQTVDFCV
TFome clones do not contain stop codon at 3'end.
CDS
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ATGTCTCCGTTGAACCAGCAACAGGTTCTCCTCAAGAAGGCCCTGGCGAAGAAGCCCAACGCTAAGAAGATGTCTGAGTTTGGCCTTAAACCCTCTGCTGCCCTCTTGAAGTCCCGGACGCAGCAGCTGCCGGCGCCGGTCCAGCCGAGGCGCCGCGTCCGTGTCCTATTTGAGGACCCCGACGCCACGGACTCGGACTCCGACTCGGACGACGAGGAGGCCGGCGGCACCCCATCAAGGACCAAGCGCTTCTCTTTTGAGGCCTTCATCGGCAAGGCCCCAGCCAAGCCGGTTCTCCCGGCGGCGGCCGCTGTCGCCGCGTGCACCAGCGGCGGGGCGCCGGAGCGCTACCGCGGAGTGAGGCTCCGCAAGTGGGGCAAGTGGGCGGCGGAGATCCGCAACCCGTTCACCGGCAAGAGGCAGTGGCTTGGCACCTTCGACACCGCTGGCGCGGCCTCCGCGGCCTACCTGTCCGCCTCCCGGAGCTTCGCCGACGAGAAGCGGCGCCGCCGCGGGCTGCCCGTGGCCGCGTCCTCAGCAGACACTCCGGCAAGCGGCAGCACCCCGACGGCTTCCTCCTCGTCGTCCGCCTCGACCGCTCCATTCGCGCACCCGTCGCCGTCGTCCGTGCTTGAACCGAGCAAGGAAGCGCGGAACACGCAGTCCCCAGAGGCGACGCCCGCCCGGCCGTCGACCGACTCAGCGCAGCTGCCGGAGGACCCAGAGTTCTACCAGGACCTTCTGCGCGGCCTGCAGCTGCCCGACATCGACCCGCTGGACTTCCGCGCCGGGCTGGACGCTCTGGATCTCTCCGATGCGGACTTCGGCCTGGACGACGAACATGACCTCCTGCTCGGGGACTTCGGGGACGAGGAGATGGAGATCGACCTCGACCTCGACGACATCAACGACGTCTTCCCGGAGATGCCCGGCTGCGACCTCGGCCGCGGCATGGACGACTTCCTGCAAACCGTTGATTTCTGCGTG
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