| TFome pUT5771 | |
|---|---|
| Template | Genomic DNA |
| Genbank Insert ID | KJ727819 |
| Protein Name | ZmEREB30 |
| Species | Zea mays |
| TF Family | AP2/ERF-AP2 |
| Gene id | GRMZM5G842961 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT5771
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | SE117_F |
| Temperature (ºC) | 77.5 |
| Sequence
|
caccATGGAAGCTGACGCACTACTCCATGCGCCCATGACTTCCTC |
3' Primer
| Name | SE117_R |
| Temperature (ºC) | |
| Sequence
|
GTCCGGCTGCACCGGCGCGGC |
PCR Condition
Step 1, 98°C 3 min melt, 1 cycle; Step 2, 98&d
Amino Acid Sequence
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MEADALLHAPMTSSSSSSSSSSDSVGGSCVVNGAQELPSNTGKPHKNVKRKRTASPSAAPGGGVAPAPPEAAIADDDCGGRRRKRGSAGTRHPTFRGVRMRVWGKWVSEIREPRKKSRIWLGTFPTAEMAARAHDVAALAIKGRGAHLNFPHLAHDLPRPASTSPADIQAAAAKAAAAAADADVEVEQCESSHAVETPSSSAASSEEVAAASNAEEMSVLFDLPDLLMDLSDGLWSPIWAAAPAAVEEYDGGDSLSEPLLWAHEHCWLDAAAPVQPD
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGAAGCTGACGCACTACTCCATGCGCCCATGACTTCCTCTTCCTCCTCTTCTTCCTCCTCCTCCGACTCCGTCGGCGGCTCGTGCGTCGTCAATGGCGCGCAGGAGCTACCGTCCAATACCGGCAAGCCGCACAAGAACGTGAAACGGAAGAGAACCGCGTCCCCGTCCGCTGCTCCAGGTGGCGGCGTGGCGCCGGCGCCGCCCGAGGCCGCCATAGCGGACGACGACTGCGGCGGCCGGCGGCGCAAGAGGGGGAGCGCCGGCACCAGGCACCCGACGTTCCGCGGCGTGCGCATGCGCGTCTGGGGCAAGTGGGTGTCCGAGATCAGGGAGCCGCGCAAGAAGTCGCGCATCTGGCTCGGCACGTTCCCGACCGCCGAGATGGCCGCGCGCGCCCACGACGTGGCGGCGCTTGCCATCAAGGGCCGCGGCGCGCACCTCAACTTCCCGCACCTCGCGCACGACCTCCCTCGCCCGGCGTCCACCTCGCCCGCCGACATCCAGGCCGCGGCAGCCAAGGCTGCTGCCGCCGCCGCCGATGCCGACGTAGAGGTAGAGCAATGCGAGTCCTCCCATGCGGTCGAGACGCCATCGTCGTCAGCCGCCAGTTCGGAGGAGGTCGCCGCGGCCAGCAACGCGGAGGAGATGAGCGTGCTGTTCGACTTGCCCGACCTTCTGATGGACCTGAGCGACGGCCTCTGGTCACCCATCTGGGCGGCAGCTCCGGCGGCGGTCGAAGAATACGACGGCGGCGACAGCCTGAGCGAGCCGCTTCTGTGGGCCCACGAACACTGCTGGCTGGACGCCGCCGCGCCGGTGCAGCCGGAC
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