| TFome pUT5955 | |
|---|---|
| Template | BT056065.1 |
| Genbank Insert ID | KJ727863 |
| Protein Name | ZmPHD16 |
| Species | Zea mays |
| TF Family | PHD |
| Gene id | GRMZM2G168249 |
| transcript | T02 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.G481C p.G161R. Present in template FLcDNA. In functional domain. |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT5955
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C389_F |
| Temperature (ºC) | 67.4 |
| Sequence
|
caccATGGATCTCGTCGCCTCC |
3' Primer
| Name | C389_R |
| Temperature (ºC) | |
| Sequence
|
GTTACCTATAACAACTAGATTTGAATTTGGTTTG |
PCR Condition
PCR Conditions: Volumes per reaction (50l): 10 l H
Amino Acid Sequence
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MDLVASPSRSKSKPRSSPAKPVAAPAESRMDLCTPSKPTPRRKSKTTASPAPMSPATPSTVRRSRRLLETPTKASPEVQVKATPISRAKRTAPSPKTPVQRETKRQRRHPRKRAYYRKVVYDGGEFEVGDDVYVKRRETAESDAEDPEEEECRVCFRTGGRVMVECDACLGGFHLRCVRPPLRRVPEGDWACPYCEAERAGKVVERPRPPEGKRVVRTAKEKLLSSDLWAARIESLWREPDGTFWAKVRWYIIPEETAAGRQLHNLRRELYRTNDLADIEMETILRHCSIMCPKDFRDANNGGDDVFYCEYEYDIHWHNFKRLADIDDEPETKEDLSDEPYNAGDDYNSDTDEDSDYDEEEESTSSFSVRRNQSHESAANSRKGRIYGLQKIGIQKIPEHARCHQKTELEKAKATLLLATLPKSLPCRDKEMDEISIFVKDAICNNQCLGRCLYIHGVPGTGKTMSVLAVMRRLRSEFDSGTLRPYCFIEINGLKLASPENIYKVVYEQLSGHRVGWKKALHYLTEHFSGGTKIGKQANQPIVLLIDELDLLMTRNQSVLYNILDWPTKPNSNLVVIGN
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGATCTCGTCGCCTCCCCCTCCAGGTCAAAATCCAAGCCCAGATCCTCGCCAGCGAAGCCCGTCGCCGCCCCCGCTGAGTCCAGGATGGACCTCTGCACCCCGTCCAAGCCCACGCCCAGGCGGAAGTCCAAAACGACGGCCTCGCCGGCGCCGATGTCTCCGGCGACGCCATCCACCGTCCGACGCTCGCGTCGCCTTCTTGAAACCCCCACCAAAGCCTCCCCGGAGGTGCAAGTGAAGGCTACGCCCATCTCCAGGGCTAAGCGCACCGCGCCGTCACCGAAAACCCCTGTTCAGCGCGAGACCAAGAGGCAGAGGCGACACCCGAGGAAGAGGGCATACTACCGGAAGGTGGTGTATGATGGCGGGGAGTTCGAGGTCGGCGATGACGTGTACGTGAAGAGGCGGGAGACGGCGGAGTCTGACGCGGAGGACCCCGAGGAGGAGGAGTGCCGCGTATGCTTCCGCACTGGCGGCCGGGTTATGGTGGAGTGCGACGCGTGCCTCGGCGGATTCCACCTGCGATGCGTGCGACCGCCGCTTCGGAGGGTGCCTGAAGGTGATTGGGCGTGCCCTTACTGCGAGGCCGAGCGCGCCGGTAAGGTGGTCGAGCGGCCCAGGCCGCCTGAGGGGAAGCGCGTCGTGAGGACCGCCAAGGAAAAACTCCTTTCCAGCGACCTGTGGGCTGCACGCATCGAGAGTTTATGGAGGGAGCCTGATGGGACGTTCTGGGCAAAGGTCAGGTGGTACATTATCCCTGAAGAGACTGCAGCAGGAAGGCAGCTGCATAACTTGAGAAGAGAGCTGTATCGTACCAATGATCTTGCAGACATTGAGATGGAAACAATCCTTAGACATTGTTCTATAATGTGCCCCAAAGACTTTAGGGATGCTAACAACGGAGGGGATGATGTATTTTATTGTGAATATGAGTATGATATACATTGGCATAATTTTAAGCGTTTGGCAGATATTGATGATGAGCCAGAGACAAAGGAAGATCTCAGTGACGAGCCCTACAATGCTGGTGATGATTATAACAGTGACACTGATGAAGATTCAGACTATGACGAGGAGGAAGAATCCACATCATCTTTCTCTGTCAGAAGGAATCAATCACATGAATCTGCGGCGAATTCAAGGAAAGGTAGAATATATGGCCTGCAAAAAATTGGGATACAAAAAATTCCTGAGCATGCTCGGTGCCACCAAAAGACTGAGCTGGAGAAGGCTAAAGCAACCCTGTTATTGGCAACTCTTCCTAAGTCATTGCCTTGCAGAGATAAAGAAATGGATGAGATATCAATATTTGTAAAGGATGCAATCTGCAACAACCAATGCCTAGGACGCTGCCTTTATATACATGGTGTACCTGGCACTGGCAAGACTATGAGTGTACTTGCTGTTATGAGGAGGCTGAGATCTGAATTTGATTCTGGAACTCTGAGGCCATATTGTTTTATCGAAATTAATGGTCTTAAATTAGCATCTCCAGAGAATATCTACAAGGTTGTATATGAACAGTTGAGTGGGCATAGGGTTGGGTGGAAAAAGGCTCTTCACTATTTAACAGAACATTTTTCAGGTGGTACTAAAATTGGAAAGCAAGCAAACCAGCCAATTGTTTTGCTGATCGATGAACTTGATCTTCTTATGACAAGAAACCAATCGGTGTTGTATAACATTCTTGACTGGCCTACCAAACCAAATTCAAATCTAGTTGTTATAGGTAAC
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