| TFome pUT5956 | |
|---|---|
| Template | Genomic DNA |
| Genbank Insert ID | KJ727864 |
| Protein Name | ZmEREB185 |
| Species | Zea mays |
| TF Family | AP2/ERF-ERF |
| Gene id | GRMZM2G028969 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT5956
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | SE0021_F |
| Temperature (ºC) | 63.9 |
| Sequence
|
caccATGGAGCACGAGCAGGTGTC |
3' Primer
| Name | SE0021_R |
| Temperature (ºC) | |
| Sequence
|
ATGTTCCCAAAGCAGCAAGG |
PCR Condition
reaction mix 5l 5x GC buffer 5l CES 1.5l MgCl 50mM
Amino Acid Sequence
Copy to clipboard
MEHEQVSPQVSDGCTCTCTYSSSSNATSGSLNGSSSPTSSDDSGGKGTNKRPRRDLKHPTYRGVRMRAWGKWVSEIREPRKKSRIWLGTFDNPEMAARAHDAAAVAIKGRAAHLNFPDLAHDLPRAASAAPKDVQAAAALAAATVPAPSPVVAPSCGHGHGLDDDADTETEEDLPPQQAMPECESGNQQAQLQLACADIGVGLGYTFLDVPDGLLDFGYMLSPLPLPAYCGSPWDDIADDLCFEEPLLLWEH
TFome clones do not contain stop codon at 3'end.
CDS
Copy to clipboard
ATGGAGCACGAGCAGGTGTCGCCTCAGGTGTCCGACGGCTGCACCTGCACCTGCACCTACAGCAGCAGCAGCAACGCCACCAGCGGCTCCCTCAACGGCTCGTCGTCGCCGACCTCCAGCGACGACTCCGGCGGCAAGGGCACGAATAAGCGGCCGCGCAGGGACCTGAAGCACCCGACGTACCGCGGCGTGCGCATGCGGGCCTGGGGCAAGTGGGTGTCCGAGATCCGGGAGCCGCGCAAGAAGTCGCGCATCTGGCTCGGCACGTTCGACAACCCGGAGATGGCCGCGCGGGCGCACGACGCCGCCGCCGTCGCCATCAAGGGCCGCGCCGCGCACCTCAACTTCCCCGACCTGGCGCACGACCTGCCCCGCGCCGCGTCCGCGGCTCCCAAGGACGTGCAGGCCGCCGCCGCGCTCGCCGCGGCCACTGTGCCGGCTCCGTCACCCGTCGTCGCCCCGTCCTGCGGCCACGGCCACGGCCTCGACGACGATGCCGACACCGAGACGGAGGAGGACCTGCCGCCTCAGCAGGCAATGCCGGAATGCGAGAGCGGGAACCAGCAGGCTCAGCTTCAGCTGGCCTGTGCCGACATTGGAGTTGGACTGGGCTATACGTTCTTGGACGTGCCAGACGGCCTTCTGGACTTCGGCTACATGTTGTCGCCGCTGCCGCTGCCGGCTTACTGTGGCTCGCCGTGGGACGACATCGCCGATGACCTCTGCTTCGAGGAGCCCTTGCTGCTTTGGGAACAT
Copyright © 2023 Grassius.org | Last updated: 2023-06-26