TFome clone pUT5978

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	P19up
Temperature (ºC)	63.5
Sequence Copy to clipboard	caccATGTTTCCTTTCTGTGATTCCTCAAGCCC

3' Primer

Name	P19dn
Temperature (ºC)
Sequence Copy to clipboard	GTAGAAGCGTGAGTTCTGCTGAAAGACGA

PCR Condition

Step 1, 98°C 3 min melt, 1 cycle; Step 2, 98&d

Amino Acid Sequence

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MFPFCDSSSPMDLPLYQQLQLSPSSPKTDQSSSFYCYPCSPPFAAADASFPLSYQIGSAAAADATPPQAVINSPDLPVQALMDHAPAPATELGACASGAEGSGASLDRAAAAARKDRHSKICTAGGMRDRRMRLSLDVARKFFALQDMLGFDKASKTVQWLLNTSKSAIQEIMADDASSECVEDGSSSLSVDGKHNPAEQLGGGGDQKPKGNCRGEGKKPAKASKAAATPKPPRKSANNAHQVPDKETRAKARERARERTKEKHRMRWVKLASAIDVEAAAASGPSDRPSSNNLSHHSSLSMNMPCAAAELEERERCSSALSNRSAGRMQEITGASDVVLGFGNGGGGYGDGGGNYYCQEQWELGGVVFQQNSRFY

TFome clones do not contain stop codon at 3'end.

CDS

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ATGTTTCCTTTCTGTGATTCCTCAAGCCCCATGGACTTACCGCTTTACCAACAACTGCAGCTAAGCCCGTCTTCCCCAAAGACGGACCAATCCAGCAGCTTCTACTGCTACCCATGCTCCCCTCCCTTCGCCGCCGCCGACGCCAGCTTTCCCCTCAGCTACCAGATCGGTAGTGCCGCGGCCGCCGACGCCACCCCTCCACAAGCCGTGATCAACTCGCCGGACCTGCCGGTGCAGGCGCTGATGGACCACGCGCCGGCGCCGGCTACAGAGCTGGGCGCCTGCGCCAGTGGTGCAGAAGGATCCGGCGCCAGCCTCGACAGGGCGGCTGCCGCGGCGAGGAAAGACCGGCACAGCAAGATATGCACCGCCGGCGGGATGAGGGACCGCCGGATGCGGCTCTCCCTTGACGTCGCGCGCAAATTCTTCGCGCTGCAGGACATGCTTGGCTTCGACAAGGCAAGCAAGACGGTACAGTGGCTCCTCAACACGTCCAAGTCCGCCATCCAGGAGATCATGGCCGACGACGCGTCTTCGGAGTGCGTGGAGGACGGCTCCAGCAGCCTCTCCGTCGACGGCAAGCACAACCCGGCAGAGCAGCTGGGAGGAGGAGGAGATCAGAAGCCCAAGGGTAATTGCCGCGGCGAGGGGAAGAAGCCGGCCAAGGCAAGTAAAGCGGCGGCCACCCCGAAGCCGCCAAGAAAATCGGCCAATAACGCACACCAGGTCCCCGACAAGGAGACGAGGGCGAAAGCGAGGGAGAGGGCGAGGGAGCGGACCAAGGAGAAGCACCGGATGCGCTGGGTAAAGCTTGCTTCAGCAATTGACGTGGAGGCGGCGGCTGCCTCGGGGCCGAGCGACAGGCCGAGCTCGAACAATTTGAGCCACCACTCATCGTTGTCCATGAACATGCCGTGTGCTGCCGCTGAATTGGAGGAGAGGGAGAGGTGTTCATCAGCTCTCAGCAATAGATCAGCAGGTAGGATGCAAGAAATCACAGGGGCGAGCGACGTGGTCCTGGGCTTTGGCAACGGAGGAGGAGGATACGGCGACGGCGGCGGCAACTACTACTGCCAAGAGCAATGGGAACTCGGTGGAGTCGTCTTTCAGCAGAACTCACGCTTCTAC