TFome clone pUT5979

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	P28up
Temperature (ºC)	62.2
Sequence Copy to clipboard	caccATGAGTGACAGCACGTTCAATTCTTGTT

3' Primer

Name	P28dn
Temperature (ºC)
Sequence Copy to clipboard	TGCTCCAGCCTTCACCCACAA

PCR Condition

Step 1, 98°C 3 min melt, 1 cycle; Step 2, 98&d

Amino Acid Sequence

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MSDSTFNSCFTHQFKLNYEDTEKVAIGYSVSSENILAGGHLWRIGCYPRGDHGKENKGECLSMFLYHESESKDAKAIFEAFVMDKEGTVSSSSHQSRVLHVFAPKGSDGYGWPSFVERSVLKSRYVTNDGSFVIVGAVQVVQEEDPLDLPPSNIGSHLGLLLDSTAGSDVTFVVDGERFAAHRAVLAARSPVFNAQLFGSMADATMTSIPLHGISAATLRAMLRFMYTDACPEEADDSDLLAAADRFDLDRLKLLCARKLWNNVTEDTVAGTLICAETYNCPQLKRKCVGFFGEGKDFKTRAVLTDDFARLALQFPSILDDLWVKAGA

TFome clones do not contain stop codon at 3'end.

CDS

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ATGAGTGACAGCACGTTCAATTCTTGTTTCACACATCAGTTTAAGCTCAATTATGAGGATACGGAGAAGGTCGCCATCGGTTACTCGGTCAGCTCCGAGAACATCCTCGCCGGTGGGCACCTCTGGAGGATCGGCTGCTACCCGCGTGGGGATCATGGGAAAGAGAACAAGGGTGAGTGCTTATCTATGTTCCTCTACCATGAGAGCGAATCCAAAGATGCCAAAGCTATCTTCGAGGCCTTTGTCATGGACAAAGAAGGTACTGTATCGTCTTCGTCCCATCAATCCAGGGTTCTGCACGTTTTCGCACCGAAGGGCAGCGACGGCTATGGTTGGCCTTCATTCGTGGAGCGAAGCGTCCTCAAGTCGCGCTACGTGACGAATGATGGATCGTTCGTCATTGTGGGAGCAGTCCAGGTTGTGCAGGAGGAGGATCCCCTCGACTTGCCACCCTCCAACATCGGGAGCCACCTCGGCCTCCTGCTGGACTCCACGGCCGGTTCCGACGTTACGTTCGTTGTCGACGGCGAGAGGTTCGCTGCCCACCGGGCCGTGCTCGCCGCCCGCTCGCCGGTCTTCAATGCGCAACTCTTCGGCTCCATGGCGGACGCCACCATGACGTCCATACCATTGCATGGAATCAGCGCAGCGACGTTAAGAGCTATGCTCCGCTTCATGTACACCGATGCTTGTCCTGAAGAAGCCGATGACTCCGACTTGCTCGCTGCTGCCGACCGCTTCGACTTGGATCGCTTGAAGCTTCTCTGCGCTAGGAAGCTGTGGAACAATGTCACGGAGGATACAGTTGCTGGTACGCTGATCTGTGCCGAAACATATAACTGCCCGCAGCTGAAAAGGAAGTGCGTTGGTTTCTTCGGCGAGGGGAAGGACTTCAAGACAAGGGCTGTGTTAACCGATGATTTTGCTCGGCTGGCACTGCAATTCCCATCGATTCTTGATGATTTGTGGGTGAAGGCTGGAGCA