| TFome pUT5980 | |
|---|---|
| Template | Genomic DNA |
| Genbank Insert ID | KJ727887 |
| Protein Name | ZmOrphan301 |
| Species | Zea mays |
| TF Family | Orphans |
| Gene id | GRMZM2G097135 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT5980
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | P33up |
| Temperature (ºC) | 64.0 |
| Sequence
|
caccATGGCGTCTCGCCGTTTCTTCTA |
3' Primer
| Name | P33dn |
| Temperature (ºC) | |
| Sequence
|
ATCGGTGTAGCACCTATTGCTCCCCTTC |
PCR Condition
Step 1, 98°C 3 min melt, 1 cycle; Step 2, 98&d
Amino Acid Sequence
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MASRRFFYDDPYDYYYTSPYHYAYPYYRYQHPAPSRGVGGFFPAAAEAAPEAVKVDPRPRVESSRSVSVPVRFVGSDPEPVRGAARMPQAAVLRKRAPSSEAAAIRLQAAARGFMARKSVRAVREVEREAAEVGEKVAREAEALRGDARARIAVGETLMKMLLRLDAVRGAREYRKRVTKRVLALQDAVDALEPKPAPDSEVVAEENESEVTSEMVDDSAEATEMPVAAEHSGGVGVNAAAETVADMEVDGGKADSEAVPEAVKESENKQDDGHINGDQTEVSNEGEWEMVADEAEPTTAATGSTEAPPQTPMTSEVMRPTGEAGAADSALDTRKVMEMVAALCEQNAQQCAVIGALAERVDALERAVRRVEDAERRRRRGKKLKKDGKGSNRCYTD
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCGTCTCGCCGTTTCTTCTACGACGACCCCTACGACTACTACTACACCTCCCCGTACCACTACGCCTACCCCTATTACCGGTACCAGCACCCCGCTCCGTCCCGCGGCGTCGGCGGGTTCTTCCCGGCCGCCGCAGAAGCCGCGCCGGAGGCGGTGAAGGTGGACCCGCGGCCCAGGGTCGAGTCTTCGAGGTCGGTCTCCGTTCCCGTCCGCTTCGTCGGTTCCGATCCGGAGCCGGTGCGCGGGGCGGCCAGGATGCCACAGGCGGCGGTGCTGAGGAAGCGGGCGCCGTCGTCGGAGGCGGCGGCGATTAGGCTGCAGGCCGCTGCGCGCGGGTTCATGGCGAGGAAGTCGGTGCGTGCTGTTCGTGAGGTGGAGCGTGAGGCGGCGGAGGTCGGAGAGAAGGTTGCGCGCGAGGCGGAGGCGCTGCGCGGGGACGCGAGGGCGCGGATCGCCGTCGGGGAGACGCTGATGAAGATGCTGCTGCGGCTCGATGCGGTGCGAGGCGCGCGGGAATACCGGAAGAGGGTCACCAAGCGGGTCCTCGCGCTGCAGGATGCTGTTGACGCCCTCGAACCAAAGCCGGCGCCGGATTCGGAGGTCGTGGCGGAGGAGAACGAGTCTGAGGTGACGTCCGAGATGGTGGACGACAGCGCGGAGGCGACGGAGATGCCGGTCGCCGCTGAGCACAGCGGCGGGGTTGGGGTGAATGCGGCGGCTGAGACGGTTGCCGATATGGAGGTGGACGGGGGCAAAGCTGACAGCGAGGCGGTGCCAGAAGCGGTGAAGGAATCCGAAAATAAGCAGGACGACGGGCACATTAACGGCGACCAGACGGAAGTCTCCAATGAGGGCGAATGGGAGATGGTGGCGGATGAAGCGGAGCCTACAACTGCGGCGACGGGTTCCACCGAGGCGCCCCCGCAGACACCGATGACGAGCGAGGTGATGAGGCCGACAGGGGAGGCCGGAGCTGCCGACAGCGCCCTGGACACTCGGAAGGTTATGGAGATGGTCGCGGCCCTGTGCGAGCAGAACGCGCAGCAGTGCGCGGTGATCGGGGCTCTGGCGGAGCGCGTGGACGCGCTTGAGCGCGCCGTGCGGCGGGTGGAGGACGCCGAGCGCCGCCGCCGGCGGGGCAAGAAGCTGAAGAAGGATGGGAAGGGGAGCAATAGGTGCTACACCGAT
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