TFome clone pUT6001

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	B912_F
Temperature (ºC)	75.0
Sequence Copy to clipboard	caccATGGCGTGCAATCGCGGCGTGA

3' Primer

Name	B912_R
Temperature (ºC)
Sequence Copy to clipboard	ACTCTTATTGGTTGACGGGGGAGAA

PCR Condition

Volumes per reaction (25l): 5 l GC buffer (Thermo

Amino Acid Sequence

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MACNRGVKEQEDPSAASAAPAPAAVARARAARPPRPRSRKGLGVRHLLKPCRFLAAVQRANARVREAGEATLVAALAAAQKESERENEVVADVCGGWKSEDGSLNCGYSSIRGRRVNMEDFYDIKSSRVDDKQINFFGVFDGHGGTHAAGYLKQHLFENLLKHPAFIGDTKSAMSQSYKKTDADFLDTEGNIHVGVGSTASTAVLIGNHLYVANVGDSRAVLSKAGKAIALSDDHKPNRSDEQKRIEDAGGVVVWSGTWRVGGILAMSRAFGNRLLKQFVVADPEIQDLEIDGDTEFLILASDGLWDVVPNEHAVAFVKDEDSPEAAARKLTEIAFRRGSTDNITCIVVEFCHDKMVDGSPPSTNKS

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGCGTGCAATCGCGGCGTGAAGGAGCAGGAGGACCCCAGCGCGGCGTCTGCAGCCCCAGCTCCGGCGGCTGTGGCTAGGGCGAGGGCGGCGCGGCCTCCGAGGCCAAGGAGCCGCAAGGGGCTCGGCGTGCGGCACCTGCTGAAGCCCTGCCGATTCCTTGCAGCTGTCCAGAGGGCGAATGCCAGGGTGAGGGAAGCGGGGGAGGCGACCCTCGTGGCAGCGCTTGCGGCGGCCCAAAAGGAGAGCGAGAGGGAGAACGAGGTGGTGGCCGATGTATGCGGCGGGTGGAAGAGTGAGGATGGAAGTTTAAATTGTGGATACTCAAGCATTAGAGGCAGAAGGGTAAACATGGAAGACTTCTATGACATAAAATCTTCTAGAGTTGATGATAAGCAAATAAATTTTTTTGGAGTATTTGATGGTCATGGAGGTACCCATGCTGCTGGGTATTTGAAACAACACTTGTTTGAAAACCTCCTAAAACATCCAGCATTCATTGGTGATACTAAGTCTGCAATGAGTCAGTCCTATAAAAAAACTGACGCAGATTTCTTGGATACTGAAGGCAATATTCATGTTGGGGTTGGGTCGACTGCATCAACAGCAGTTTTGATTGGCAACCATCTGTATGTGGCCAATGTTGGTGATTCAAGGGCTGTACTATCAAAGGCAGGCAAAGCTATTGCACTCTCTGATGACCATAAACCAAACAGAAGTGATGAGCAGAAACGGATTGAGGATGCTGGAGGAGTTGTTGTTTGGTCTGGAACATGGAGAGTAGGTGGTATACTTGCAATGTCTAGGGCATTTGGCAATCGTTTGTTGAAGCAGTTCGTTGTTGCAGACCCTGAGATTCAGGATCTAGAAATAGATGGTGATACGGAGTTTCTTATTTTGGCCAGTGATGGGCTCTGGGATGTGGTGCCAAATGAGCATGCTGTTGCATTTGTGAAGGACGAGGATAGCCCTGAGGCTGCAGCCCGGAAGCTTACGGAGATCGCCTTCAGGCGTGGGAGCACCGACAACATCACATGCATTGTTGTAGAATTTTGCCACGATAAGATGGTTGATGGTTCTCCCCCGTCAACCAATAAGAGT