TFome clone pUT6021

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	C501_F
Temperature (ºC)	69.8
Sequence Copy to clipboard	caccATGTCACTTGCTGTACCTGTGACACTAA

3' Primer

Name	C501_R
Temperature (ºC)
Sequence Copy to clipboard	GTGGCCGGCAGCAGCTTGGCT

PCR Condition

Volumes per reaction (25l): 5 l GC buffer (Thermo

Amino Acid Sequence

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MSLAVPVTLKTTQSGENERLEYAVSAMQGYRLNMEDAHAIVLNLDAATGTSFFGVYDGHGGPAVSKYCARHLHAELRRHESFRDNLQTAIERTFLRMDEMMRDRRAGRELSGYGGNDNWKAYRKAINMSLFLPFCQKPAYQGPVMDGCTACVVLIRDNRIIVGNAGDSRCVLSRNNQAIDLSTDFKPNLPDERQRIEAAGHVVTFSERGNVHRIDDGIAVSRSLGDLMYKDNNDLGPVQQAITAFPEVRTEEITQDDQFLIIACDGIWDCLTSQQAVDFIRIYSFADVGLASICEALLAHCVAQPRGRDNMTVILVRFKTPGACQVRASNVPPPPIRQDAAASQAAGSQAAAASQAAGSQAAAGH

TFome clones do not contain stop codon at 3'end.

CDS

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ATGTCACTTGCTGTACCTGTGACACTAAAGACTACTCAGAGCGGTGAGAATGAAAGACTTGAATACGCCGTGTCAGCTATGCAAGGATACCGTTTAAATATGGAGGATGCTCATGCAATTGTCCTGAATCTTGATGCTGCAACTGGCACATCATTCTTTGGTGTTTATGATGGCCATGGAGGACCTGCTGTTTCAAAGTATTGTGCGAGACACCTACACGCTGAGCTTCGCCGACATGAAAGTTTTCGGGATAATCTCCAGACTGCAATTGAGAGAACGTTCTTAAGGATGGATGAGATGATGAGAGACAGGAGGGCAGGGAGGGAATTATCTGGGTACGGTGGTAATGACAATTGGAAAGCATATAGAAAGGCTATCAACATGAGTCTATTTCTACCCTTCTGTCAGAAGCCAGCTTATCAGGGGCCAGTAATGGATGGATGTACCGCGTGTGTGGTTCTCATTAGAGACAACCGAATCATTGTGGGAAATGCTGGTGATTCTCGTTGTGTACTCTCAAGGAATAATCAGGCGATTGATCTATCCACCGATTTTAAACCAAACCTTCCAGACGAAAGACAAAGAATAGAAGCTGCAGGACATGTGGTAACTTTTAGCGAGAGAGGAAATGTGCATCGTATTGATGATGGAATTGCGGTTTCGAGATCACTTGGTGACCTAATGTACAAGGATAATAATGATTTAGGTCCCGTACAACAAGCAATTACTGCCTTTCCTGAAGTTCGCACTGAAGAGATAACTCAAGATGACCAGTTTCTTATTATAGCATGTGATGGAATCTGGGATTGCCTAACAAGCCAGCAAGCAGTTGATTTTATAAGGATATACTCATTTGCTGACGTAGGGCTGGCGTCCATCTGCGAGGCCCTCCTTGCTCACTGCGTGGCACAACCTAGAGGGAGGGACAACATGACTGTCATACTGGTTCGGTTCAAGACGCCAGGCGCTTGCCAAGTTAGGGCCAGCAACGTACCGCCACCGCCGATCCGCCAAGATGCTGCTGCGAGCCAAGCTGCTGGGAGCCAAGCTGCTGCTGCGAGCCAAGCCGCTGGGAGCCAAGCTGCTGCCGGCCAC