| TFome pUT6047 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ727949 |
| Protein Name | ZmTUB14 |
| Species | Zea mays |
| TF Family | TUB |
| Gene id | GRMZM2G301122 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | Cloned with stop codon. |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT6047
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
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| Temperature (ºC) | |
| Sequence
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3' Primer
| Name | |
| Temperature (ºC) | |
| Sequence
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PCR Condition
Amino Acid Sequence
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MEGNNLPSGSLMQGTPYSNVDLQHHKHMQMPAPSSGNQAFNHSQVPGNFPICLNQVTDSDQLPEFQFGEHGKVNHHHHPHHHKQHATNSMSDDEEHGVHEDATDSQIGKGKKGSTWHRMKWTDSMVRLLITAASYTGEDPGADLGGGRRSCAMMQKKGKWKAISKVMGERGCLVSPQQCEDKFNDLNKRYKRLTDILGRGTTCRVVANPELLDGMANLSDKMKDAARKILSSKHLFYEEMCSYHNNNWFSLPEDPALQRSLQLALKCKDEHDARKRASGDADEDDQSADTDYEEENDDEHPITHANKGTLLTRKRLRYMPADMEDVGFGNSSSSHDCSWRSDPHSIAVDINKASPDGTNLASVQKDLATQSAEIEKQRMEIEVEALELAKQRLKWEAFSKKKDRELEKMRLENEQMKIENKRLELEVRYKELELEHKPQGHGSHAT*
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGAAGGAAATAACCTACCCTCTGGAAGCTTGATGCAAGGAACACCTTACAGTAATGTGGACTTGCAGCATCATAAACATATGCAAATGCCTGCACCAAGCTCTGGAAACCAGGCTTTCAACCACTCTCAGGTGCCTGGCAATTTCCCCATTTGTCTGAACCAGGTTACAGATTCCGACCAGCTACCTGAATTCCAATTTGGAGAGCACGGCAAGGTCAATCACCACCATCATCCTCACCATCACAAGCAGCATGCAACGAATAGCATGAGCGATGATGAGGAGCATGGTGTGCATGAGGATGCCACTGATAGCCAGATCGGCAAGGGAAAGAAGGGTTCCACATGGCATCGGATGAAGTGGACTGATTCGATGGTTAGGCTTTTGATTACTGCAGCATCCTACACTGGGGAAGACCCAGGAGCTGATTTAGGAGGTGGTAGGAGAAGCTGTGCAATGATGCAGAAGAAAGGCAAGTGGAAGGCCATATCAAAAGTCATGGGCGAGCGAGGCTGCCTCGTGTCGCCGCAGCAGTGTGAGGATAAGTTCAATGACCTAAATAAGAGGTATAAAAGGCTTACAGATATCCTTGGTCGGGGTACTACTTGCAGAGTTGTGGCGAACCCGGAACTTCTGGATGGCATGGCTAATCTCTCAGATAAGATGAAAGATGCCGCGAGGAAGATATTGAGCTCTAAGCACTTGTTCTATGAAGAGATGTGCTCCTACCACAACAATAATTGGTTCAGTTTACCTGAAGATCCTGCACTTCAGCGATCGTTGCAGCTTGCTCTTAAGTGTAAGGATGAGCATGATGCAAGGAAGCGAGCAAGTGGAGATGCTGATGAAGATGATCAAAGTGCAGACACTGATTATGAAGAAGAGAATGATGATGAGCATCCCATCACGCATGCCAATAAGGGGACCCTACTCACGCGTAAAAGATTGCGGTACATGCCGGCGGATATGGAGGATGTAGGTTTCGGCAACTCTTCCAGCTCACATGACTGTAGCTGGAGATCTGATCCCCATAGCATTGCAGTGGACATCAACAAAGCTTCTCCAGATGGAACTAACTTGGCTTCGGTTCAAAAAGACTTGGCTACACAGTCTGCGGAGATTGAGAAACAGCGTATGGAAATTGAAGTTGAGGCATTGGAGCTTGCAAAACAACGTCTCAAGTGGGAGGCATTTAGTAAGAAGAAGGACAGAGAGCTAGAGAAGATGAGGTTGGAAAATGAACAAATGAAGATTGAGAATAAACGTTTGGAGCTAGAGGTAAGATATAAGGAGTTGGAGCTTGAACACAAACCACAAGGCCATGGCAGTCATGCAACATGA
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