| TFome pUT6076 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ727964 |
| Protein Name | ZmARF1 |
| Species | Zea mays |
| TF Family | ARF |
| Gene id | GRMZM2G169820 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.C2092T p.L698F conserved SNP. Not present in functional domain. |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT6076
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | R7-F |
| Temperature (ºC) | 69.8 |
| Sequence
|
caccATGGAGGCGCCGGGGACGAG |
3' Primer
| Name | R7-R |
| Temperature (ºC) | |
| Sequence
|
GTCGCCGGTGACCCTCCACG |
PCR Condition
Amino Acid Sequence
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MEAPGTSSGGAGGEGAGGTKVNEELWYACAGPLVALPPAGSLVVYFPQGHSEQVAASMRKDADAKIPSYPNLSSKLICILRSVTMLADPDTDEVYARMTLQPVSNVTHCDKETLLATELALKQTRPQTEFFCKTLTASDTSTHGGFSVPRRAAERIFPHLDFSVQPPAQELQARDLHDAIWTFRHIYRGQPKRHLLTTGWSLFVSGKRLLAGDSVLFIRDGRQQLLLGIRRANRQPVNLSSSVLSSDSMHIGILAAAAHAAANNSQFTVFYNPRASPSEFVIPFAKYQKAVYSNQLSLGMRFRMMFETEESATRRYMGTITGISDMDPLRWKNSQWRNIQVAWDEAAPSERRTRVSLWEVEPVIAPFFIYPSPLFTAKRPRQPGVTDDDSSEMDNLFKRTMPWFGEEVGKRDLSTQNGLVPGLSLVQWMNMQHNPSLANTVMQPELLNSLAGKPVQTLAAADLSRQISFQPQFLQQNNIQFNTSLLPPQNQQTEQLANVIATPNQLGSVIVPQKAVQNSSSEQKQNLVTQPLQVSQPMVSVAQPQLVHTQLQQPQVVLQAQPQQPQVIVQAQLQQQQPLVQNHTTIQSGLQQIQLLQQQQVQQSVQEQQQIKIQPFQVPNDANMVTQLSDQMKIQLLKALQPQQPLVMEQQKMIFDLQQQAVNSQSTAQQCSQVATQVVGLHSSSTNQYPTQQKTQPFKTFQDFPGNAVSIVKPEIVTSVGARSLHVPGGVQSMKMDEVPSSSTSPSTNNNPVILRSTPSSSKNQCLPTAAKAPQSSVVLGSTLEQGMKPCDSTQHPMVIPKLAEERPATGQDYMNSTQLDYLDTSSSATSVCLSQADGSLQQSFPPSSFNQHQPLREAVPDSEFEVTDAGNNFLFGANIDGHMEPLNEDDLLGTAFEADKYMEQMPGNGISNYISSKDSQQELSSSMISHPFGVADIAFNSIDSSINDIQFLNRNSRAPGPVQQRMRTYTKVHKRGAVGRSIDINRYSGYDELKHDVARMFGIEGQLSDQNRVGWKLVYEDHEKDVLLVGDDPWEDFVNCVRCIRILSPQEERQMRLASDYGDSFLGNQACSSSDGGRPWRVTGD
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGAGGCGCCGGGGACGAGCTCGGGCGGCGCCGGAGGCGAGGGGGCCGGGGGCACCAAGGTCAACGAGGAGCTGTGGTACGCGTGCGCGGGACCGCTCGTGGCGCTGCCGCCGGCGGGGAGCCTCGTTGTCTACTTCCCGCAGGGACACAGCGAGCAGGTAGCAGCATCTATGCGAAAGGATGCAGATGCCAAAATTCCGAGCTATCCAAATCTCTCATCTAAGCTTATATGCATACTCCGTAGTGTCACTATGCTTGCTGATCCTGACACAGATGAGGTTTACGCTCGAATGACTCTCCAGCCCGTTAGCAATGTGACGCATTGTGACAAGGAGACGTTGCTGGCAACAGAGCTTGCACTGAAACAAACCAGACCACAGACAGAGTTCTTTTGTAAAACACTGACTGCAAGTGATACAAGCACTCATGGAGGTTTCTCTGTGCCACGGCGTGCTGCAGAGCGAATTTTCCCCCATCTTGACTTCTCAGTGCAGCCTCCTGCTCAGGAACTGCAGGCCAGGGATTTGCACGATGCAATTTGGACATTCCGTCATATATATCGAGGCCAGCCTAAAAGGCATTTGCTGACTACTGGCTGGAGCCTATTTGTCAGTGGAAAGAGACTCCTTGCTGGTGATTCAGTCCTGTTTATTAGGGACGGAAGACAGCAACTCCTCTTGGGGATAAGGCGAGCAAATAGGCAGCCCGTTAACCTCTCATCATCTGTTTTGTCTAGCGACAGTATGCATATTGGAATTCTTGCTGCTGCAGCCCATGCAGCAGCCAATAACAGCCAATTCACAGTATTCTATAACCCAAGGGCTAGTCCTTCAGAATTTGTTATTCCTTTTGCCAAGTACCAGAAGGCCGTCTATAGCAACCAACTATCACTTGGCATGCGGTTTAGAATGATGTTTGAAACTGAAGAATCTGCAACAAGAAGGTACATGGGAACAATAACTGGTATAAGTGATATGGATCCTTTAAGGTGGAAAAACTCCCAATGGCGCAATATTCAGGTTGCATGGGATGAAGCAGCACCAAGTGAGAGACGCACCAGGGTTTCCCTTTGGGAGGTTGAACCTGTTATCGCTCCATTCTTCATTTATCCCTCGCCATTGTTCACTGCAAAACGTCCGAGGCAACCTGGGGTAACAGATGATGATAGCTCAGAAATGGACAATCTTTTTAAGCGGACCATGCCATGGTTTGGTGAGGAGGTTGGCAAGAGAGATCTGAGTACTCAAAACGGTTTAGTTCCTGGATTAAGTTTGGTTCAGTGGATGAATATGCAACACAACCCCTCTCTTGCCAACACGGTTATGCAACCGGAATTGCTAAATTCGTTAGCTGGAAAACCTGTACAAACTCTGGCTGCGGCTGATCTATCTAGGCAAATTAGCTTCCAGCCCCAGTTCCTGCAACAAAATAACATCCAGTTCAACACTTCGCTGCTACCTCCCCAAAACCAGCAGACCGAACAGTTAGCAAATGTAATAGCTACACCAAACCAATTGGGAAGTGTTATAGTACCACAAAAGGCAGTTCAAAATTCCAGTTCTGAACAGAAACAGAATCTGGTTACACAACCACTGCAAGTCAGCCAACCAATGGTTAGTGTGGCACAGCCTCAACTTGTCCATACTCAACTCCAGCAACCTCAAGTAGTTCTCCAGGCTCAGCCTCAGCAACCTCAAGTCATTGTCCAAGCTCAACTACAACAACAGCAACCTCTGGTTCAAAATCACACCACCATACAAAGTGGCCTTCAACAAATCCAGCTTCTGCAGCAACAGCAAGTTCAGCAGTCAGTGCAGGAACAACAGCAAATAAAGATACAGCCTTTTCAGGTACCTAATGATGCCAACATGGTAACACAGTTATCTGATCAGATGAAAATACAACTATTGAAGGCTCTACAGCCACAGCAGCCTCTGGTCATGGAACAGCAGAAAATGATATTTGATTTGCAGCAACAAGCAGTAAATTCTCAGTCAACTGCTCAGCAGTGCTCACAGGTAGCTACCCAAGTGGTTGGTTTGCATAGCAGTAGCACTAATCAGTACCCGACGCAGCAAAAGACTCAACCTTTCAAAACATTTCAAGATTTTCCTGGGAATGCTGTTTCTATTGTAAAACCAGAAATTGTCACCTCCGTGGGTGCTCGCTCTTTGCATGTGCCTGGTGGAGTGCAGTCAATGAAGATGGATGAAGTTCCCTCTTCTTCAACATCGCCATCCACAAACAACAATCCTGTTATTTTGCGATCAACTCCAAGTAGCTCCAAGAACCAATGTTTACCTACTGCAGCAAAGGCTCCTCAGTCATCTGTTGTATTGGGTTCTACACTTGAACAGGGTATGAAGCCTTGTGACAGTACACAACACCCAATGGTGATTCCCAAGCTGGCTGAAGAAAGGCCAGCTACTGGACAAGACTACATGAACAGCACTCAGCTGGATTATTTGGATACGTCATCTTCAGCGACTTCAGTTTGCCTTTCACAGGCTGACGGATCGCTGCAACAAAGTTTTCCACCTTCATCCTTCAATCAGCATCAACCGTTGAGGGAAGCAGTTCCAGATAGCGAGTTTGAGGTTACAGATGCAGGAAATAACTTTCTATTTGGGGCAAATATTGATGGCCATATGGAGCCTCTTAACGAAGACGATTTGCTTGGAACTGCCTTCGAAGCTGACAAGTACATGGAACAGATGCCAGGAAACGGCATCTCTAACTACATTTCATCAAAGGATTCTCAGCAAGAGTTATCATCTTCAATGATCTCACATCCATTTGGCGTTGCTGATATCGCGTTCAATTCCATAGATTCGTCGATCAATGATATCCAATTCTTAAACAGAAATTCCCGGGCTCCAGGTCCTGTTCAACAACGTATGCGAACATACACCAAGGTGCACAAGCGTGGCGCTGTTGGAAGATCTATCGACATCAACAGATATTCTGGTTACGATGAACTGAAGCATGACGTTGCCCGTATGTTTGGAATTGAGGGGCAGCTCAGCGACCAAAACAGAGTTGGCTGGAAACTGGTTTACGAAGATCACGAGAAGGATGTCCTATTAGTTGGTGATGACCCATGGGAGGACTTCGTGAACTGCGTGCGGTGCATCAGGATTCTTTCGCCGCAGGAAGAGAGGCAAATGAGGCTGGCCAGCGACTACGGAGACAGCTTTCTTGGCAACCAAGCGTGTAGCAGCTCGGACGGGGGTCGTCCGTGGAGGGTCACCGGCGAC
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