TFome pUT6078
Template RT-PCR
Genbank Insert ID KJ727965
Protein Name ZmARF21
Species Zea mays
TF Family ARF
Gene id GRMZM2G390641
transcript T01
Vector pENTR/SD/D-TOPO
Note c.G25C p.A9P; c.C681T p.(=); c.C1034T p.A345V Not present in functional domain.
Request Information Deposited to ABRC
Subgenome: 2

TFome clone pUT6078

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name
Temperature (ºC)
Sequence
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3' Primer

Name
Temperature (ºC)
Sequence
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PCR Condition

Amino Acid Sequence
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MITFVDSAAMELERESGRCLVDPQLWHACAGGMVQMPPVHSRVYYFPQGHAEHAQGHAHADLPAGRVPALVLCRVDAVRFLADPDTDEVLARVRLAPVRPNEPDHADAAAPGAREDKPASFAKTLTQSDANNGGGFSVPRYCAETIFPRLDYSADPPVQTVLAKDVHGVVWKFRHIYRGTPRRHLLTTGWSAFVNQKRLVAGDSIVFMRTGNGDLCVGIRRAKKGGIGGGPEFPHHQPPDGGGYGYGYAGFSTFLRGEEDDAAARGKVRVLVRPEEVVEAANLAASGQPFEVVYYPRASTPEFCVKAGAVRAAMRTQWCAGMRFKMAFETEDSSRISWFMGTVAAVQVADPIRWPNSPWRLLQVAWDEPDLLQNVKRVSPWLVELVSSTPAIHHLTPFSPPSRKKLCIPLYPEGHQLPAPMFHGSPLVGRGVGPMRYFPDGGTPPAGIQGARHAQFGISLPDLHHLTRLQSSLSPHAHGLRHQLDHGARPRIAGGLIVGHPAARDDISCLLTIGTAPHKKPSDVKSAAAAPAPQLMLFGKPILTEQQISLGFRPLPAPKKSPSDDAAETERTVSNNSDASSPAGTASGSTPSISGGAPSSCQDNKAAATATATDDDDLLGHCKVFMQSEDVGRTLDLSAVASYEELYQRLADMFGVDRAELTSHVFYRDGASGALKHAGDEPFSEFTKTARRLTIQTDAR*

TFome clones do not contain stop codon at 3'end.

CDS
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ATGATTACTTTCGTGGACTCGGCGGCCATGGAGCTGGAGAGGGAGAGCGGCAGGTGCCTGGTGGACCCGCAGCTGTGGCACGCCTGCGCCGGCGGCATGGTCCAGATGCCGCCGGTGCACTCCAGGGTGTACTACTTCCCGCAGGGCCACGCCGAGCACGCGCAGGGCCACGCCCACGCCGACCTCCCCGCCGGCCGGGTCCCGGCCCTCGTCCTCTGCCGCGTCGACGCCGTCCGGTTCCTGGCCGATCCGGACACCGACGAGGTGCTCGCCAGGGTCCGCCTCGCCCCCGTCCGGCCCAACGAGCCGGACCACGCCGACGCCGCGGCGCCGGGCGCGCGGGAGGACAAGCCCGCGTCCTTCGCCAAGACGCTCACGCAGTCCGACGCCAACAACGGCGGGGGCTTCTCCGTGCCGCGGTACTGCGCCGAGACCATCTTCCCGCGGCTGGACTACTCCGCCGACCCGCCCGTCCAGACCGTGCTCGCCAAGGACGTGCACGGCGTCGTCTGGAAGTTCCGCCACATCTACCGCGGCACGCCCCGGCGCCACCTGCTCACCACCGGGTGGAGCGCCTTCGTGAACCAGAAGAGGCTCGTCGCGGGGGACTCCATCGTGTTCATGAGGACGGGGAACGGGGACCTCTGCGTCGGCATCCGGCGCGCCAAAAAGGGCGGCATCGGAGGAGGGCCGGAGTTCCCGCACCACCAGCCGCCAGACGGCGGCGGGTACGGGTACGGGTACGCCGGCTTCTCCACGTTCCTGAGAGGAGAAGAGGACGACGCGGCCGCGAGGGGCAAGGTCAGGGTGCTGGTGCGGCCGGAGGAGGTCGTCGAGGCCGCCAACCTCGCGGCGAGCGGGCAGCCGTTCGAGGTGGTCTACTACCCGAGGGCGAGCACGCCGGAGTTCTGCGTCAAGGCGGGGGCGGTCCGCGCGGCCATGCGCACCCAGTGGTGCGCCGGGATGAGGTTCAAGATGGCCTTCGAGACGGAGGACTCGTCCAGGATCAGCTGGTTCATGGGCACTGTCGCCGCCGTGCAGGTCGCTGACCCCATCCGATGGCCCAATTCACCCTGGAGGCTTCTTCAGGTCGCTTGGGATGAACCGGACTTGTTACAGAACGTGAAGAGAGTGAGCCCATGGCTGGTCGAGCTTGTCTCAAGCACGCCGGCCATCCACCACCTCACGCCGTTCTCGCCGCCAAGCAGGAAGAAGCTGTGTATCCCATTGTACCCGGAGGGCCACCAGCTCCCGGCGCCGATGTTCCACGGAAGCCCTCTGGTGGGCCGTGGTGTTGGCCCGATGCGCTACTTCCCGGACGGCGGCACTCCTCCTGCAGGCATACAGGGAGCCAGGCATGCGCAATTCGGCATATCTTTACCAGATCTCCACCACCTCACCAGGCTGCAGTCGAGTCTATCGCCGCACGCGCACGGGCTTCGCCACCAGCTCGACCACGGCGCGCGGCCGAGGATCGCCGGCGGCCTCATCGTCGGCCACCCGGCAGCTCGGGACGACATCTCGTGCTTGCTCACCATTGGCACCGCCCCGCACAAGAAACCATCAGACGTCAAGTCGGCCGCGGCGGCGCCGGCGCCGCAGCTGATGCTGTTTGGGAAACCGATTCTCACCGAGCAGCAGATATCTCTAGGCTTTCGGCCGCTGCCGGCGCCGAAGAAGAGCCCGTCCGACGACGCTGCTGAGACTGAGAGGACGGTGAGCAACAACTCGGATGCCTCGAGCCCAGCTGGGACTGCGAGTGGGAGCACGCCGTCCATCAGTGGAGGCGCCCCGTCGTCGTGCCAGGACAACAAGGCGGCGGCGACGGCGACGGCGACTGATGACGACGATCTCCTCGGGCACTGCAAGGTGTTCATGCAGTCGGAGGACGTCGGGCGCACGCTCGACCTCTCGGCTGTCGCGTCGTACGAGGAGCTCTACCAGAGGCTGGCCGACATGTTCGGCGTCGACAGGGCCGAGCTGACGAGCCATGTCTTCTACCGCGACGGCGCTTCTGGAGCGCTCAAGCACGCCGGCGACGAGCCTTTCAGTGAGTTCACCAAGACCGCTCGGAGGCTGACCATACAAACGGACGCGAGGTAG


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