| TFome pUT6079 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ727966 |
| Protein Name | ZmbHLH48 |
| Species | Zea mays |
| TF Family | bHLH |
| Gene id | GRMZM2G027068 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT6079
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | R22-F |
| Temperature (ºC) | 64.9 |
| Sequence
|
caccATGTTCCCGGTCGAAGTGTCGG |
3' Primer
| Name | R22-R |
| Temperature (ºC) | |
| Sequence
|
TGCTGCCGCTTGCATGTTCATG |
PCR Condition
Amino Acid Sequence
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MFPVEVSALAAGGAGAGRQAAGDAAGTMLPPFFMGSIWPATAGAAGSEEDEAAAAAAAHDRALAASRNHREAEKRRRERIKSHLDRLRNVLACDPKIDKASLLAKAVERVRDLKQRAAGVGEAAPAHLFPTEHDEIVVLASGSGAVFEASVCCDDRSDLLPDLIETLRALRLRTLRSEMATLGGRVRNVLVLARDVVDGGGGAVAGGDDGYGGRAADSAGGATDGGGGDFLKEALRALVERPGAAGDRPKRRRVSDMNMQAAA
TFome clones do not contain stop codon at 3'end.
CDS
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ATGTTCCCGGTCGAAGTGTCGGCGCTCGCGGCGGGCGGTGCGGGAGCGGGGCGTCAGGCTGCCGGCGACGCCGCCGGGACGATGCTGCCGCCCTTTTTCATGGGCTCCATCTGGCCCGCGACCGCTGGCGCCGCTGGCTCCGAGGAGGACGAGGCGGCGGCCGCCGCCGCCGCCCACGACCGCGCGCTCGCCGCGTCGCGCAACCACCGCGAGGCCGAGAAGCGCCGTCGCGAGCGCATCAAGTCGCACCTCGACCGCCTCCGCAACGTCCTCGCCTGCGACCCCAAGATAGACAAGGCTTCGCTGCTGGCGAAGGCGGTGGAGAGGGTGCGGGACCTGAAGCAGCGGGCGGCGGGCGTCGGGGAGGCAGCGCCGGCGCACCTGTTCCCGACGGAGCACGACGAGATCGTTGTGCTCGCGTCGGGCAGCGGCGCCGTCTTCGAGGCCTCCGTCTGCTGCGACGACCGCTCCGACCTCCTCCCGGACCTCATCGAGACGCTGCGCGCGCTCCGCCTCCGCACGCTCCGCTCCGAGATGGCCACCCTCGGCGGCCGCGTCCGCAACGTGCTCGTCCTGGCGCGCGACGTCGTCGACGGCGGCGGCGGTGCCGTCGCGGGAGGTGATGACGGATACGGTGGACGCGCTGCTGACTCGGCGGGCGGCGCCACGGACGGCGGCGGCGGCGACTTCCTCAAGGAAGCCCTGCGGGCTCTGGTAGAGAGGCCAGGCGCCGCCGGAGACCGGCCCAAGAGGCGACGGGTCTCGGACATGAACATGCAAGCGGCAGCA
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