| TFome pUT6084 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ727970 |
| Protein Name | ZmABI19 |
| Species | Zea mays |
| TF Family | ABI3-VP1 |
| Gene id | GRMZM2G035701 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT6084
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | R44-F |
| Temperature (ºC) | 66.7 |
| Sequence
|
caccATGGCCGGCATTACCAAGCGC |
3' Primer
| Name | R44-R |
| Temperature (ºC) | |
| Sequence
|
CATCTGAGGCCCGGATTTTGG |
PCR Condition
Amino Acid Sequence
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MAGITKRRTSPASTSSSSGDVLPQRVTRKRRSARRGPRSTARRPSAPPPMNELDLNTAALDPDHYATGLRVLLQKELRNSDVSQLGRIVLPKKEAESYLPILMAKDGKSLCMHDLLNSQLWTFKYRYWFNNKSRMYVLENTGDYVKAHDLQQGDFIVIYKDDENNRFVIGAKKAGDEQTATVPQVHEHMHISAALPAPQAFHDYAGPVAAEAGMLAIVPQGDEIFDGILNSLPEIPVANVRYSDFFDPFGDSMDMANPLSSSNNPSVNLATHFHDERIGSCSFPYPKSGPQM
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCCGGCATTACCAAGCGCCGCACCTCCCCGGCCTCCACCTCCTCTTCGTCCGGCGACGTCTTGCCGCAGCGGGTCACCCGGAAGCGTCGGTCCGCCCGCCGCGGGCCCCGGAGCACCGCCCGTAGGCCGTCGGCGCCTCCACCTATGAATGAACTGGACTTGAATACAGCTGCTCTTGATCCGGATCATTATGCTACAGGATTGAGAGTTCTTCTTCAGAAGGAGCTCCGAAATAGCGATGTAAGCCAGCTTGGGAGAATTGTTCTCCCAAAGAAGGAGGCGGAGTCTTACCTCCCTATTCTGATGGCAAAGGATGGAAAGAGTTTATGCATGCATGACTTGCTAAATTCACAACTGTGGACCTTCAAGTATAGATATTGGTTCAACAACAAAAGCAGGATGTATGTGCTTGAAAATACCGGAGATTATGTAAAAGCTCATGACCTTCAGCAAGGAGACTTCATCGTGATCTACAAGGACGACGAGAACAACCGCTTTGTCATAGGAGCAAAGAAGGCAGGAGATGAGCAGACCGCCACTGTACCTCAAGTCCATGAACACATGCACATCTCTGCCGCACTGCCAGCTCCACAAGCGTTCCATGACTATGCAGGCCCCGTCGCAGCAGAAGCTGGTATGCTCGCGATCGTGCCACAGGGTGACGAGATATTCGACGGCATACTGAACTCCCTGCCGGAGATACCAGTGGCGAACGTGAGGTACTCCGACTTCTTCGACCCGTTCGGTGACTCCATGGACATGGCGAATCCGCTGAGCTCCTCCAATAACCCCTCGGTCAACCTGGCTACGCATTTCCATGACGAGAGGATCGGGAGCTGCTCGTTTCCCTACCCAAAATCCGGGCCTCAGATG
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