| TFome pUT6085 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ727971 |
| Protein Name | ZmGRAS67 |
| Species | Zea mays |
| TF Family | GRAS |
| Gene id | GRMZM2G049159 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT6085
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | R46-F |
| Temperature (ºC) | 61.1 |
| Sequence
|
caccATGGGTCTGGATAATACTTATGGCGA |
3' Primer
| Name | R46-R |
| Temperature (ºC) | |
| Sequence
|
GTTATTCTTAGGCTTCCAGGTAGATATTGC |
PCR Condition
Amino Acid Sequence
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MGLDNTYGDLSGMFYGGLSYDGYTDHSSASDYFRFADPLPATVPQMAPEACSNPSSTVSRANTETDNPEDWEFISDESLDYISRMLMEEDIDEKVSMYQAESAALRAAAKPFYDILGHKFPPSPDRQLISWSMDSPSESSSSICTQSVASTVTSSSVGGTVDSNRRYDIGLSEQLEAYRGLCGQASHPLVGTSSDASNAVDVLEDPLITNGQIPEYLFESLPTWDFRRGIEEAQKFLPVNNKLVIGLEAGGIARPQEARKDVSLNAKKADALKVKKNRQSEDLDVIEGRNIKQSAFCSDEPDWIEMFDDLLLQTEKKATDLRELMRNEASKNSQVTQTKGPSGPRPRGRKPTKKDVVDLRTILIHCAQAVAADDRRTANELLKQIRHHSKPNGDGTQRLAHCFADGLEARLAGTGSQLYRKLIAKRTTASDMLKAYHLYLAACPFKRLSHFLSNQTILSMTKHASKVHIIDFGIYFGFQWPCLIRRLSKREGGPPVLRITGIDVPQPGFRPTERIEETGQRLAEYAEKLKVPFEYQGIASKWETIRVEDLKVGKDEVVIVNCLYRFRNLIDETVAVDSPRNRVLNTIRQVNPAIFIHGIVNGSYSVPFFITRFREALFHFSALFDMLETTVPRDDAQRALIEREMFGREALNVIACEGSDRVERPETYKQWQVRNLRAGFVQSPLNQEIVMKAKDKVKDIYHKDFVIDEDSGWLLQGWKGRIIYAISTWKPKNN
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGGTCTGGATAATACTTATGGCGACCTATCTGGCATGTTCTATGGAGGCCTGTCTTATGATGGCTACACAGACCACAGCAGCGCAAGTGATTACTTCAGATTTGCCGATCCACTTCCTGCCACTGTGCCCCAGATGGCACCAGAAGCCTGTTCAAATCCTTCCTCCACTGTGTCAAGGGCCAATACTGAAACTGACAACCCAGAGGATTGGGAATTCATCTCAGATGAGTCCCTCGACTATATCAGCCGGATGCTCATGGAGGAGGATATTGATGAGAAAGTTAGCATGTACCAGGCGGAGTCAGCAGCACTGCGTGCTGCTGCAAAGCCGTTCTATGACATTCTTGGGCACAAGTTCCCACCGTCTCCTGACCGCCAGCTGATCTCTTGGTCCATGGACAGCCCCAGTGAGAGTAGCAGTAGCATCTGCACACAATCTGTGGCAAGTACTGTCACGAGCAGCAGTGTTGGTGGCACAGTGGATAGCAACCGGCGCTATGACATTGGACTTAGTGAGCAGCTGGAAGCTTACCGAGGCTTGTGTGGTCAAGCTTCTCATCCACTTGTTGGTACATCAAGTGATGCTTCCAATGCAGTGGATGTGCTAGAAGACCCTTTGATCACGAACGGTCAGATCCCTGAGTATTTGTTTGAGAGCCTTCCAACTTGGGATTTCAGGAGAGGTATTGAGGAAGCCCAGAAGTTTCTTCCTGTTAACAATAAACTAGTCATTGGTTTAGAAGCTGGTGGTATTGCAAGACCCCAAGAGGCAAGGAAAGATGTTTCTTTGAATGCCAAAAAGGCAGATGCATTGAAGGTCAAGAAAAATAGACAGAGTGAAGACCTTGACGTGATTGAAGGCCGGAACATTAAACAGTCTGCATTTTGTTCCGATGAACCTGATTGGATTGAAATGTTCGATGATCTGCTTCTTCAAACCGAGAAGAAGGCCACAGATCTGCGAGAGCTGATGCGGAATGAAGCTTCCAAGAATTCTCAGGTCACCCAGACGAAAGGACCAAGTGGACCACGGCCACGGGGAAGGAAACCAACAAAGAAGGATGTGGTGGACCTTAGGACCATCCTCATCCACTGTGCACAGGCTGTGGCAGCAGATGACCGCAGGACTGCTAATGAATTGTTAAAGCAAATAAGGCACCATTCCAAGCCAAATGGTGATGGAACCCAGAGGTTAGCACATTGCTTTGCAGATGGTCTTGAGGCTCGATTGGCAGGCACAGGGAGTCAGCTTTACCGCAAGCTTATAGCAAAACGAACAACTGCCTCAGACATGCTTAAAGCTTACCACCTTTACCTTGCGGCATGCCCATTTAAGAGGCTTTCACATTTTCTTTCCAATCAAACAATCTTGAGTATGACTAAACATGCATCAAAGGTGCATATCATTGACTTCGGCATTTATTTCGGCTTCCAATGGCCATGCCTAATCAGGCGTCTCTCCAAGAGGGAAGGTGGTCCACCTGTTCTGCGCATTACTGGAATTGATGTACCTCAGCCAGGTTTCCGACCTACTGAGAGAATTGAAGAGACTGGACAAAGGCTTGCAGAATATGCTGAGAAGTTGAAGGTGCCTTTTGAGTATCAGGGAATAGCATCTAAGTGGGAAACCATCCGAGTTGAGGATCTCAAAGTTGGCAAGGACGAAGTTGTGATTGTTAATTGCCTATACAGATTCAGAAACCTGATTGATGAAACAGTTGCTGTAGACAGTCCTAGGAATAGAGTGCTCAACACCATAAGGCAAGTAAACCCAGCAATTTTCATTCATGGAATTGTGAATGGGTCATACAGTGTTCCCTTCTTCATCACACGTTTCCGTGAGGCGCTGTTCCATTTCTCTGCATTGTTTGACATGCTAGAGACAACTGTCCCACGGGATGATGCACAGCGTGCACTCATAGAGAGGGAAATGTTTGGCCGAGAGGCACTCAATGTTATTGCATGTGAGGGCTCAGACAGAGTTGAGAGACCCGAGACATATAAACAGTGGCAGGTGAGGAACCTCAGGGCTGGATTTGTTCAGTCCCCGTTAAACCAAGAAATCGTGATGAAAGCCAAGGACAAAGTAAAAGACATTTATCACAAGGACTTTGTCATAGATGAAGACAGCGGATGGCTCCTGCAAGGCTGGAAAGGAAGGATAATCTATGCAATATCTACCTGGAAGCCTAAGAATAAC
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