| TFome pUT6096 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ727981 |
| Protein Name | ZmVOZ5 |
| Species | Zea mays |
| TF Family | VOZ |
| Gene id | GRMZM2G449165 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.G957A p.(=) PZE08173003000 c.G1069A p.A357T Conserved SNP. Not present in functional domains. |
| Request Information | Deposited to ABRC |
TFome clone pUT6096
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | R153F |
| Temperature (ºC) | 64.5 |
| Sequence
|
caccATGACTCATGAGACGACGGCAGTCT |
3' Primer
| Name | R153R |
| Temperature (ºC) | |
| Sequence
|
TGTCCCATCATTTGAGTTCCAATAGAAGT |
PCR Condition
Amino Acid Sequence
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MTHETTAVSVSRCYWTALSALTSTDALIRMLGPAVAFPKAADPDDDPTKWQPENSAEHLACETKDGAPEEGKGKQPPRPRPFRSTSTRAQRSRRRMRMRMGKGPSSRAGSRHRQFRARAMTRVDDLQEVFSGLQSARKDSRSADAAVLEAQLQQMLREWRSELSDPSPASSLQGNARELSDPPSDTLRLLQLVVAEEEDDATSKMVEQQQQQPPPPLPANRSQGHAHVVCQGMKPEPREEAVDVAVDQPQLLSQGVLPNGAATASAMFHDQLYYVNQELTVEDFLYDDDYKIDLSGSNLDVINNLEGIVQLEYPDHNLPQGLAPNAYLDMNSCGQSAGAVFLHMPDLLTTMTSAPSTFLKPKCALWDCPRPAIGSERWHNYCSMYHADLAVQEEGPPGTMPVIRPRGIDLKDGPLFAALSAKVQGKNVGVPICEGAATAKSPWNAPELFDLYIFEGESIREWLFFDKPRRAFDSGNRKQRSLPDYNGRGWHESRKQVMKDFGGLKRSYYMDPQPSNGYEWHLYEYEINDCDAFALYRLEFKSSDAKKIAKSKLGCNPLNEIQQQMDRLSADSPVDTKRTARSRTEPNPIDVNTNIYSVPNTTVQTDVPNAYQPGSQVDQMTYLNGSVVYGPHLPYGYSTERSDFYWNSNDGT
TFome clones do not contain stop codon at 3'end.
CDS
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ATGACTCATGAGACGACGGCAGTCTCCGTCTCCCGCTGTTATTGGACGGCGCTTTCGGCATTGACGAGCACCGATGCCCTAATCCGCATGCTGGGCCCGGCCGTCGCTTTTCCCAAGGCCGCCGATCCCGACGACGACCCAACAAAGTGGCAGCCAGAAAACTCGGCAGAGCATCTCGCTTGCGAAACAAAGGACGGCGCGCCAGAGGAGGGGAAAGGAAAACAACCACCACGACCCCGTCCATTCCGTTCCACTTCCACCCGAGCTCAGCGATCGCGGCGGCGGATGCGGATGCGGATGGGGAAGGGGCCGTCGTCGCGGGCGGGGTCGCGGCACCGGCAGTTCCGCGCCCGAGCCATGACCCGCGTCGACGACCTCCAGGAGGTCTTCTCGGGCCTGCAGTCCGCGCGCAAGGACTCACGCTCCGCGGACGCCGCCGTCCTCGAGGCGCAGCTCCAGCAGATGCTGCGGGAGTGGCGCTCCGAGCTCAGCGATCCCTCCCCCGCCTCCTCCCTGCAGGGCAACGCCAGGGAACTGTCGGATCCGCCTTCCGATACTCTTCGTCTGCTGCAGTTGGTGGTGGCCGAGGAGGAGGATGACGCCACGAGCAAGATGGTCGAGCAGCAGCAGCAGCAGCCCCCACCTCCGCTCCCAGCTAACCGGAGCCAGGGGCACGCGCATGTTGTGTGCCAGGGCATGAAGCCGGAGCCGCGCGAGGAAGCAGTTGACGTCGCTGTGGACCAGCCGCAGCTGCTGAGTCAGGGGGTTCTGCCTAATGGTGCTGCTACCGCGTCTGCGATGTTCCATGATCAGCTGTACTATGTGAACCAGGAGCTCACGGTCGAGGATTTTCTTTATGATGATGATTACAAGATAGATCTTTCTGGGTCTAATCTGGATGTCATTAATAACCTAGAAGGGATTGTTCAGCTGGAATATCCAGATCACAATTTGCCACAAGGGTTAGCCCCTAATGCATACCTTGACATGAATAGTTGTGGGCAGAGTGCCGGGGCTGTTTTCCTGCACATGCCAGATTTGCTCACAACAATGACTTCAGCACCTTCTACATTCCTGAAGCCAAAATGCGCTCTCTGGGACTGCCCCAGGCCTGCTATTGGATCAGAAAGATGGCATAATTATTGCAGCATGTATCATGCTGATTTGGCTGTGCAGGAAGAGGGCCCTCCAGGCACAATGCCTGTGATCCGTCCAAGAGGCATTGATCTGAAAGATGGCCCCCTGTTTGCTGCTCTTAGTGCAAAAGTCCAAGGAAAAAACGTTGGGGTCCCTATCTGTGAAGGAGCTGCCACTGCAAAGTCTCCATGGAATGCACCTGAACTTTTCGATCTTTACATATTTGAAGGTGAATCTATCAGAGAATGGTTGTTTTTTGACAAACCAAGGAGGGCATTTGATAGTGGAAATCGGAAGCAGCGGTCCTTGCCAGACTACAACGGCCGTGGTTGGCATGAATCTAGGAAGCAGGTGATGAAGGACTTTGGGGGTCTGAAGAGGTCCTATTACATGGATCCACAACCATCCAACGGCTATGAATGGCACCTATATGAATATGAGATCAATGACTGTGATGCTTTTGCCTTATATCGGCTTGAATTCAAGTCTTCAGATGCAAAGAAAATTGCAAAATCCAAATTAGGTTGTAATCCACTGAATGAAATTCAGCAGCAAATGGATAGACTCAGTGCAGACAGTCCTGTCGACACCAAACGGACGGCCCGGAGCCGGACAGAGCCTAACCCGATAGATGTCAATACTAACATCTATTCAGTTCCAAACACCACAGTTCAAACTGATGTTCCAAATGCTTATCAGCCAGGGTCACAAGTGGACCAGATGACATATTTGAACGGTAGTGTCGTTTATGGGCCTCATCTGCCGTACGGTTACTCGACTGAAAGAAGTGACTTCTATTGGAACTCAAATGATGGGACA
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