| TFome pUT6118 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ727997 |
| Protein Name | ZmC2H3 |
| Species | Zea mays |
| TF Family | C2H2 |
| Gene id | GRMZM2G023988 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | Start codon 374bp downstream of T01 start codon. Supported by RNA-seq. |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT6118
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | R138F |
| Temperature (ºC) | 65.8 |
| Sequence
|
caccATGGACTCCACCAGGAGCAACCA |
3' Primer
| Name | R138R |
| Temperature (ºC) | |
| Sequence
|
ATGTCCATCTGCTGCTGGTCCAG |
PCR Condition
Amino Acid Sequence
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MDSTRSNQVAGRCNSTIASRAATVPPSLTMPRGELADAIVGGGGGGGADGDPRAALLRLSALGDRMAAVRGRICASISGEARPLSYADIQSVSSEISAAAQLVVLNAAELLASSVPFPAPPPPPPSAAPSPAPVREIPVVAASAQEQPLEAAKDDGGYEVVELDAAELLAEHVHFCEICGKGFRRDANLRMHMRAHGDRFKTLDALSRPGHGQPKPPVGSNVRFSCPFAGCNRNRTHRRFRPLKSAVCARNHFRRSHCPKLYACERCGGKKRFAVLADLRSHLRHCGEEAQWRCSCGTTFSRKDKLFGHLALFEGHTPAVTKPNKDVVTGPTESTIDAMEEGGFEEGIPDREEDEEGGYDPEFFKEWMDELRGGAGGSNWPGPAADGH
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGACTCCACCAGGAGCAACCAGGTCGCCGGCAGATGCAACAGCACCATCGCGTCAAGGGCTGCCACGGTCCCGCCGTCGCTGACCATGCCGCGCGGCGAACTCGCTGACGCCATCGTGGGCGGAGGCGGAGGAGGAGGAGCGGACGGGGACCCCCGGGCCGCCCTCCTCCGTCTGTCTGCTCTCGGGGACCGCATGGCCGCCGTCCGGGGCCGTATCTGCGCCTCTATCTCGGGCGAAGCCCGGCCTCTCTCCTACGCCGACATCCAATCTGTCTCCTCCGAGATCTCCGCCGCTGCCCAGCTCGTCGTCCTCAACGCCGCCGAGCTCCTCGCTTCCTCCGTCCCCTTTCCCGCCCCACCTCCACCTCCTCCTTCCGCCGCTCCCTCTCCCGCTCCCGTCCGAGAGATCCCAGTTGTGGCCGCTTCCGCCCAAGAGCAACCCCTGGAGGCCGCCAAGGACGACGGTGGCTACGAGGTCGTGGAGCTCGACGCCGCCGAGCTGCTAGCGGAGCACGTCCACTTCTGCGAGATCTGCGGCAAGGGCTTCCGCCGCGACGCCAACCTCAGGATGCACATGCGCGCCCACGGCGACCGCTTCAAGACCCTAGACGCGCTCTCCCGCCCTGGTCACGGCCAGCCGAAGCCGCCCGTTGGCAGCAACGTGCGCTTCTCCTGCCCCTTTGCGGGCTGCAACAGGAACCGCACACACCGCCGCTTCCGGCCGCTCAAGTCGGCGGTGTGCGCGCGGAACCACTTCCGGCGCAGCCACTGCCCCAAGCTCTACGCCTGCGAGCGATGTGGCGGCAAGAAGCGCTTCGCTGTCCTGGCGGACCTCCGCAGCCACCTCCGCCACTGCGGCGAGGAGGCACAGTGGCGCTGCTCCTGCGGCACCACCTTCTCCCGCAAGGACAAGCTCTTCGGTCATCTAGCGCTCTTTGAAGGCCACACGCCAGCAGTTACTAAGCCAAACAAGGATGTGGTGACAGGGCCTACAGAGTCCACCATTGATGCTATGGAGGAAGGGGGATTTGAAGAAGGCATTCCTGACCGGGAGGAGGATGAAGAGGGCGGTTATGATCCAGAGTTCTTCAAGGAGTGGATGGATGAGCTCAGAGGTGGTGCTGGTGGCTCCAATTGGCCTGGACCAGCAGCAGATGGACAT
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