| TFome pUT6133 | |
|---|---|
| Template | Genomic DNA |
| Genbank Insert ID | KJ728002 |
| Protein Name | ZmZIM4 |
| Species | Zea mays |
| TF Family | ZIM |
| Gene id | GRMZM2G036351 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT6133
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | R158F |
| Temperature (ºC) | 70.4 |
| Sequence
|
caccATGGCCATGGCGGCAGCGGAC |
3' Primer
| Name | R158R |
| Temperature (ºC) | |
| Sequence
|
CAGCGCGATGGTGAGGCGGTCG |
PCR Condition
Amino Acid Sequence
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MAMAAADGKSRRFALACGVLSQYVKAEQQQQQQMAAPRAPATTTLSLMPGADVGADEQQEAAAAGAEEMPGPAPAAAPPLTIFYGGRVVVFEDFPAEKAAEVMRLAAGDDLPIARKASLQRFLAKRKDRLVERAPYARPSSPAEAVKPASGSASASWLGLRSTEADRLTIAL
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCCATGGCGGCAGCGGACGGCAAGAGCCGGAGGTTCGCGCTGGCCTGCGGGGTGCTGAGCCAGTACGTCAAGGCCGAGCAGCAGCAGCAGCAGCAGATGGCGGCCCCGCGCGCCCCGGCCACCACGACCCTGAGCCTGATGCCCGGCGCGGACGTCGGCGCCGACGAGCAGCAGGAGGCGGCCGCCGCTGGGGCCGAGGAGATGCCCGGGCCCGCCCCCGCCGCGGCGCCGCCGCTCACCATCTTCTACGGCGGCAGGGTGGTGGTGTTCGAGGACTTCCCGGCGGAGAAGGCGGCCGAGGTCATGCGCCTCGCGGCCGGCGACGACCTGCCCATCGCCCGGAAGGCGTCGCTGCAGCGGTTCCTGGCCAAGCGCAAAGATCGCCTCGTCGAGCGCGCGCCCTACGCCCGCCCGTCGTCCCCCGCGGAGGCGGTCAAGCCGGCCTCGGGCTCGGCCTCGGCCTCCTGGCTCGGGCTCCGCAGCACGGAAGCCGACCGCCTCACCATCGCGCTG
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