| TFome pUT6146 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ728011 |
| Protein Name | ZmZIM30 |
| Species | Zea mays |
| TF Family | ZIM |
| Gene id | AC197764.4_FG003 |
| transcript | T003 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT6146
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | R156F |
| Temperature (ºC) | 65.8 |
| Sequence
|
caccATGTCGGCCGCCACTGGTTAC |
3' Primer
| Name | R156R |
| Temperature (ºC) | |
| Sequence
|
AAGCCCGAGCCATGAGGCAG |
PCR Condition
Amino Acid Sequence
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MSAATGYSRFLRQYMREPQRHQLVEAEETDSGRTMQLFPTRAGGGTSSSSHEQSDDGAQAANKATLSIFYEGRMLVFEEFPADKAKALMQLAAGSSGSSSAAAPNNKDAPVPVVRVRRVPEQPAATAPLAVVPSDLLPIARKVSLQRFLQKRKERIAATEPYPEPEKDDVPATATATATGWQATALKDKPAPASWLGL
TFome clones do not contain stop codon at 3'end.
CDS
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ATGTCGGCCGCCACTGGTTACAGCCGGTTCCTGCGGCAGTACATGAGAGAGCCGCAGCGGCATCAGCTGGTGGAGGCGGAGGAGACGGACTCCGGCCGGACCATGCAGCTGTTCCCCACGCGCGCCGGCGGCGGCACGTCGTCGTCGTCGCATGAACAGTCAGATGATGGTGCGCAGGCGGCCAACAAGGCAACGCTGAGCATCTTCTACGAGGGCCGGATGCTCGTGTTCGAGGAGTTCCCGGCCGACAAGGCCAAGGCGCTGATGCAGCTGGCCGCCGGGTCGTCGGGCTCCTCGTCGGCGGCGGCGCCCAATAACAAGGACGCCCCAGTACCAGTGGTGCGGGTGCGGCGGGTGCCAGAGCAGCCGGCAGCCACCGCGCCATTGGCAGTCGTGCCCTCCGACCTGCTGCCCATCGCGAGGAAAGTGTCGCTGCAGAGGTTCCTCCAGAAGAGGAAGGAGAGGATCGCCGCCACCGAGCCTTACCCGGAGCCGGAGAAAGATGATGTTCCTGCCACCGCCACCGCCACCGCCACCGGCTGGCAAGCGACGGCCTTAAAGGATAAACCTGCACCTGCCTCATGGCTCGGGCTT
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