| TFome pUT6152 | |
|---|---|
| Template | Genomic DNA |
| Genbank Insert ID | KJ728016 |
| Protein Name | ZmGRAS6 |
| Species | Zea mays |
| TF Family | GRAS |
| Gene id | GRMZM2G346706 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT6152
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | Dup12 |
| Temperature (ºC) | 55.8 |
| Sequence
|
caccATGGACAACCTTGAGTACTGTGA |
3' Primer
| Name | Ddn12 |
| Temperature (ºC) | |
| Sequence
|
TGAATCTTTAGGTTTCCATGACG |
PCR Condition
Amino Acid Sequence
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MDNLEYCEINSDITLNYITQILMEESADENISAYKHHDALQAMEKPFYDILGKAYPSSSKQTMVNSGSQSGLPDDINNSHHDLERSGNSVSDLLRGRKGVRSIGIDGGSELCDVALQFNRIAEEAKKFVPSIGNLVVDPESNDLSSSKEANGSAIGQHSNQTNKIRSYPHVDLKLVEGRNSKYSAISTSEIIRNELFDRVLLSDEQYLCDVAHLREMAKEANINLQYVQNTGSAQGKQKPQSKKQEKEAVDLRGLLIQCAQAISSNNHPFASELLKKIRHHSSPYGDGSQRLAVYFADALEARAAGTGSQINQRLVVKRTSVTDMLKAYRLSIAACPFGRVAYYFANKTIVDVLGSRPRVHIIDFGIMFGFQWPSLIQRLAKREGGPPQLRITGIDVPETGFRPCKQIEETGKRLAEYARMFNVPFQYQSVASRWESIYIADLNIGRDEVLIVNCLHKMKNLGDETEDIDSARDRVLRIMKRMNPDVLITGVMNGLHSSPFFLPRFREALFFYSSQFDMLNSTVVHQNHEARIMIERDLLGADVFNVVACEGAERIERPESYKQWQARILKAGFKKLPVDQTILKGSVDRKELYHGDFVIDEDSGWLLQGWKGRIMHALSSWKPKDS
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGACAACCTTGAGTACTGTGAGATAAACTCGGACATAACTCTCAACTATATAACCCAAATATTGATGGAAGAAAGCGCTGACGAGAATATTAGCGCATACAAACATCACGATGCACTCCAGGCCATGGAGAAGCCCTTCTACGATATTCTTGGAAAGGCATATCCATCCTCATCTAAACAGACTATGGTCAACAGTGGTAGTCAATCAGGTCTGCCTGATGATATTAACAACAGTCATCACGACCTGGAACGCAGTGGTAACTCTGTCAGTGATCTTCTTCGTGGGCGCAAGGGGGTGCGCTCGATAGGCATAGATGGCGGTTCTGAACTTTGCGATGTGGCTTTGCAGTTCAACAGAATTGCTGAGGAAGCAAAGAAGTTTGTTCCAAGTATTGGGAACTTGGTGGTTGATCCAGAGAGCAATGACCTTTCCAGTTCCAAAGAGGCAAATGGGTCAGCAATTGGGCAACATAGCAATCAAACAAATAAAATAAGGAGCTACCCTCACGTGGACTTGAAGTTGGTGGAGGGGAGAAACAGTAAGTATTCAGCAATCTCAACCAGTGAAATAATTCGGAATGAACTATTTGACAGAGTTCTGCTCTCTGATGAGCAGTATCTTTGCGATGTTGCTCACCTTAGAGAAATGGCAAAAGAAGCAAACATAAATCTGCAGTATGTTCAGAACACAGGATCTGCCCAAGGAAAACAGAAGCCACAAAGTAAGAAGCAAGAAAAGGAAGCGGTTGATCTCAGGGGTCTTCTCATCCAGTGTGCACAAGCGATATCATCTAACAACCATCCATTTGCCAGTGAGCTGCTGAAAAAGATCAGGCACCATTCTTCACCATATGGAGATGGTTCTCAGAGGCTGGCAGTTTACTTTGCAGATGCTCTTGAGGCACGTGCAGCTGGGACTGGGAGTCAAATAAACCAGAGATTAGTGGTGAAGCGAACAAGCGTCACAGACATGCTGAAGGCCTACCGCCTTTCCATCGCAGCATGCCCTTTCGGCAGGGTAGCATACTACTTCGCCAATAAAACAATTGTTGATGTGTTGGGGAGTCGACCAAGGGTGCATATAATTGATTTTGGCATCATGTTTGGCTTTCAGTGGCCATCGCTAATCCAGCGGCTTGCAAAGCGAGAAGGTGGTCCCCCTCAACTTCGCATCACAGGTATTGACGTGCCCGAGACAGGTTTTCGCCCCTGTAAACAGATTGAGGAGACAGGAAAGCGGTTGGCTGAGTATGCAAGAATGTTCAATGTACCTTTCCAGTATCAAAGTGTTGCCTCACGGTGGGAAAGCATATACATAGCAGATCTCAATATTGGCAGGGATGAGGTGCTCATAGTCAACTGCTTGCACAAGATGAAAAACCTCGGCGATGAGACAGAAGACATAGACAGTGCAAGGGACAGGGTACTGCGTATCATGAAGAGGATGAATCCAGATGTTCTCATAACCGGTGTCATGAATGGGTTGCACAGCTCCCCGTTCTTCCTACCACGATTCAGAGAGGCTTTGTTCTTTTACTCATCACAGTTTGACATGCTAAATTCAACTGTTGTGCATCAGAATCATGAGGCAAGGATCATGATAGAGAGGGATCTCCTTGGGGCAGATGTGTTTAATGTTGTAGCATGTGAAGGTGCAGAGAGGATTGAGAGGCCAGAGAGCTACAAACAATGGCAAGCAAGAATCCTCAAGGCTGGGTTCAAGAAACTTCCTGTTGACCAGACCATTCTAAAGGGTTCAGTAGACAGAAAGGAGCTTTATCATGGTGACTTTGTCATCGACGAAGACAGTGGCTGGTTGCTACAGGGATGGAAAGGAAGGATAATGCATGCGCTGTCGTCATGGAAACCTAAAGATTCA
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