| TFome pUT6169 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ728030 |
| Protein Name | ZmbHLH79 |
| Species | Zea mays |
| TF Family | bHLH |
| Gene id | GRMZM2G173862 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.G774A p.(=) |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT6169
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | R38-F |
| Temperature (ºC) | 67.5 |
| Sequence
|
caccATGGATCCGTTCTACTTCGGCCCGGAC |
3' Primer
| Name | R38-R |
| Temperature (ºC) | |
| Sequence
|
ATCCATCTGGACAGTTTCGACTTGACAGCTG |
PCR Condition
Amino Acid Sequence
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MDPFYFGPDQLDAAAVDEDYLTSLGLVVLSPEPVAPLPGGAFEAYQRRAPALLEHSLQTMSRRYSGEHNMHRRMFSYLRRVAHDAGAAVVAAPAFPAPARESVPGFGVGTSQAPRSSRFRNIMRERLRRERLSQGFADLHALLPHGASSKGGKNDIVGAAAGYVRELVARKEQLSARNQELLEKAASRWKQQGGTRSGVGRGMVVKVQAESQDHSMALGAFVRVLQRLKAMEAGLQVTAIRTRFSAGGVWMNIGVEGQVSARDVDKSITNALIELEGNDPRSSKPRFSCQVETVQMD
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGATCCGTTCTACTTCGGCCCGGACCAGCTGGATGCCGCCGCCGTCGACGAGGACTACCTGACGAGCCTCGGCCTGGTGGTCCTCTCGCCGGAGCCAGTGGCGCCGTTGCCTGGCGGCGCATTTGAGGCGTACCAGCGGCGTGCGCCGGCGTTGCTGGAGCACTCCCTGCAGACGATGAGCCGGCGGTACAGTGGAGAGCACAACATGCACCGGAGGATGTTCAGTTACCTGAGACGCGTAGCTCATGACGCCGGCGCCGCCGTGGTGGCGGCCCCAGCGTTCCCGGCGCCCGCGCGCGAGAGCGTTCCCGGCTTCGGGGTTGGCACGTCGCAGGCGCCGCGAAGCTCGCGGTTCCGGAACATCATGCGCGAGCGGCTGCGGCGGGAGCGCCTCAGCCAGGGATTCGCCGACCTCCACGCTCTTCTGCCCCACGGCGCGTCGTCAAAAGGTGGCAAGAACGACATCGTCGGCGCGGCGGCCGGCTACGTCAGGGAGCTGGTGGCCAGGAAGGAGCAGCTCAGCGCCAGGAACCAGGAGCTCCTAGAGAAGGCCGCGAGCCGCTGGAAGCAGCAGGGAGGCACGAGGAGTGGTGTCGGGCGGGGCATGGTGGTGAAGGTGCAAGCCGAGAGCCAAGACCATTCGATGGCGCTTGGTGCGTTTGTGAGGGTGCTCCAGCGGCTCAAGGCCATGGAGGCCGGGCTGCAGGTGACGGCGATCCGGACGCGCTTCTCCGCGGGAGGCGTGTGGATGAACATCGGAGTGGAAGGCCAAGTATCCGCTCGTGATGTGGACAAGTCAATAACAAACGCCTTGATAGAGCTGGAGGGGAACGATCCAAGAAGCAGCAAGCCCAGATTCAGCTGTCAAGTCGAAACTGTCCAGATGGAT
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