| TFome pUT6192 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ728046 |
| Protein Name | ZmZIM7 |
| Species | Zea mays |
| TF Family | ZIM |
| Gene id | GRMZM2G063632 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.A69G p.(=). c.G75A p.(=). c.129ins69. Support in RNA-seq. c.C334A p.R112S. c.T348C p.(=). c.C264G p.H88Q. In functional domain, the rest are not. |
| Request Information | Deposited to ABRC |
TFome clone pUT6192
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | R160F |
| Temperature (ºC) | 62.5 |
| Sequence
|
caccATGGTGGATCAGTATGTGTATGACTCGG |
3' Primer
| Name | R160R |
| Temperature (ºC) | |
| Sequence
|
GACTTCAGACTTCGCGGCTCCC |
PCR Condition
Amino Acid Sequence
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MVDQYVYDSVPPEKAQAIMLIAAAAAAAAATKGSAATAFNPPMSTSVAAGQAQVVADPSSISKLQADLPIARRHSLQRFLEKRRDRVVSKAPYSPAKSFDGMESAGMEMTADGKQGARPSILKGWGAAKSEV
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGTGGATCAGTATGTGTATGACTCGGTGCCACCAGAAAAGGCTCAGGCAATCATGCTTATAGCTGCGGCTGCAGCAGCTGCGGCAGCCACCAAAGGCAGTGCTGCCACTGCTTTTAATCCTCCAATGAGCACTTCTGTAGCAGCTGGACAAGCTCAGGTTGTTGCTGACCCTAGCTCAATAAGCAAGCTTCAGGCTGATCTCCCCATTGCCAGGAGGCACTCTCTCCAGCGCTTCCTTGAGAAACGTCGTGACAGGGTCGTGAGCAAAGCTCCGTACAGCCCCGCCAAGTCGTTCGATGGCATGGAGTCAGCGGGAATGGAGATGACTGCGGACGGCAAGCAGGGCGCAAGGCCCAGTATCTTGAAAGGTTGGGGAGCCGCGAAGTCTGAAGTC
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