| TFome pUT6434 | |
|---|---|
| Template | BT035923.1 |
| Genbank Insert ID | KJ728164 |
| Protein Name | ZmEREB80 |
| Species | Zea mays |
| TF Family | AP2/ERF-ERF |
| Gene id | GRMZM2G151542 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.C521T p.A174V Present in functional domain. c.C528T p.(=); SNPs not present in template FlcDNA |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT6434
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B379_F |
| Temperature (ºC) | 66.3 |
| Sequence
|
caccATGTCGCGCTCGCGCAC |
3' Primer
| Name | B379_R |
| Temperature (ºC) | |
| Sequence
|
GAAAATCGCGGGAAGCCGGTTTAG |
PCR Condition
PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer f
Amino Acid Sequence
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MSRSRTVRIFWDDPDLTDSSGDDEGCGARRVGRMVRELAPAPAPAPAALPVPDQCNPGDGDRGRKAGVAGPGVCSGARRRLAKGAGGAASTKFRGVRRRPWGKFAAEIRDPWRGVRVWLGTFDTAEEAARVYDAAAVQLRGANATTNFSASAATNCGSGSGAGAGQDPATTPAVGYESGAESSPTSVLRKVPSLSSLAEDDSDAPAAQRRSLAVLEEEELGEFVPFEDAPVYATSGFWDFEPDAGFLYAEPSSPEASWNAAAAVAEPASSDDAPSWAASPVQENDYFQDLRDLFPLNRLPAIF
TFome clones do not contain stop codon at 3'end.
CDS
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ATGTCGCGCTCGCGCACGGTGCGGATCTTCTGGGACGATCCTGACCTGACCGATTCCTCCGGCGACGACGAGGGCTGCGGAGCCCGGAGGGTCGGCAGAATGGTGAGGGAGCTGGCACCAGCACCAGCACCAGCACCGGCGGCTCTTCCCGTGCCGGATCAGTGCAACCCCGGGGACGGCGACCGCGGGAGGAAGGCCGGCGTCGCTGGTCCCGGCGTGTGCAGCGGGGCGCGGAGACGGCTGGCCAAGGGCGCCGGTGGCGCGGCGAGCACCAAGTTCCGCGGCGTGCGGAGGCGGCCGTGGGGCAAGTTCGCGGCGGAGATCCGCGACCCGTGGCGCGGCGTGCGCGTCTGGCTCGGCACCTTCGACACCGCGGAGGAGGCCGCACGCGTCTACGACGCCGCCGCCGTCCAGCTCCGCGGGGCCAACGCCACCACCAACTTCTCCGCGTCCGCCGCCACTAACTGCGGCTCCGGCTCCGGGGCCGGGGCCGGGCAGGACCCGGCGACGACACCGGCGGTGGGCTATGAGTCCGGCGCGGAGTCGTCGCCGACGTCCGTGCTCCGCAAGGTCCCCTCCCTGTCCTCCCTCGCCGAGGACGACTCGGACGCGCCGGCCGCCCAGCGCCGCAGCCTGGCCGTCCTCGAGGAGGAGGAGCTGGGCGAGTTCGTGCCGTTCGAGGACGCGCCCGTCTACGCCACCAGCGGCTTCTGGGACTTCGAGCCCGACGCGGGGTTCCTCTACGCCGAGCCGTCGTCGCCGGAGGCGTCGTGGAATGCCGCCGCCGCCGTCGCCGAGCCCGCCTCGTCTGACGACGCGCCCAGCTGGGCCGCGTCGCCGGTGCAGGAGAACGACTACTTCCAGGACCTGCGCGACCTCTTCCCGCTAAACCGGCTTCCCGCGATTTTC
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