| TFome pUT6441 | |
|---|---|
| Template | BT056185 |
| Genbank Insert ID | KJ728171 |
| Protein Name | ZmOrphan36 |
| Species | Zea mays |
| TF Family | Orphans |
| Gene id | GRMZM2G020156 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT6441
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B947_F |
| Temperature (ºC) | 64.6 |
| Sequence
|
caccATGAAGTACATGAAGCTCGGTTCA |
3' Primer
| Name | B947_R |
| Temperature (ºC) | |
| Sequence
|
CTGCTTGTTCCTCATCCTGCTC |
PCR Condition
Volumes per reaction (25l): 5 l GC buffer (Thermo
Amino Acid Sequence
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MKYMKLGSKPDTFYTEEAVRSVVSDIPADLIIHVNNTKYQLHKFPLLLKCGLLQRLCSDTDDGDEEPVPVALHDIPGGEQAFELCAKFCYGISISISAGNLVPAMLAARFLRMTEAVAKGNLVAKLETFFDSCVLQGWKDSVAALQAAWRISGWNESRIVQPCIDSIVEKILLPPSKVTWSYTYTRPGYNSTRKPHQSVPKDWWTEDISDLDIEVFRSVISTVRASRLLPPPLIGEALHVYACKHLVDPLHVASDAGLTANGVLHQPPQSSDDGEEKLVAMQRRVLESIVTMVPGEPGSVTGRFLLRLLRVANYVSASSSTRAQLIRQAGSQLDEARAADLLIPLPSDPHAYDVGTAEAVLEHFLAQFQRPAPTDERRRMSAAMEKVARIFDEYLRTIALDRSFPVGKFVDLVECLPDIARSDHDGLYYAIDIYLKEHPELSKADKKRLCRLIDCRKLSPDVRAQAIANDRMPLRTIVQLLFVEQERTIGAAAGSHAASGGAADRASVGVISQLAAGTKDEEPASSAGHKSDVHRPRRDHARVADGASAMARSLSASTKTPPAARKERTPEETGSRMRNKQ
TFome clones do not contain stop codon at 3'end.
CDS
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ATGAAGTACATGAAGCTCGGTTCAAAACCTGATACGTTCTATACCGAAGAAGCAGTCAGGTCAGTCGTATCAGACATACCCGCCGATCTCATTATCCATGTAAATAACACCAAGTATCAGCTACACAAGTTCCCACTGCTCCTCAAGTGCGGCCTCCTGCAGCGCCTCTGCTCCGACACCGACGACGGCGATGAGGAGCCGGTGCCCGTGGCGCTGCACGACATCCCTGGCGGCGAGCAGGCCTTTGAGCTGTGCGCCAAGTTCTGCTACGGCATCTCCATCAGCATCAGCGCCGGGAACCTGGTGCCCGCGATGCTGGCGGCGCGCTTCCTCCGGATGACGGAGGCCGTCGCCAAGGGCAACCTGGTGGCCAAGCTCGAGACCTTCTTCGACTCCTGCGTCCTGCAGGGCTGGAAGGACTCGGTCGCCGCGCTGCAGGCCGCGTGGCGGATCTCCGGCTGGAACGAGAGCCGCATCGTACAGCCCTGCATCGACTCCATCGTCGAGAAGATCCTCCTGCCACCGTCCAAGGTCACGTGGTCCTACACGTACACGAGGCCAGGGTACAACAGCACGAGGAAACCCCACCAGTCCGTCCCCAAGGACTGGTGGACGGAGGACATCTCCGACCTGGACATCGAGGTCTTCCGCTCCGTCATCTCCACCGTGCGCGCGTCGCGGCTGCTCCCACCGCCGCTCATCGGCGAGGCGCTGCACGTCTACGCGTGCAAGCACCTCGTCGACCCGCTCCACGTTGCCAGCGACGCAGGACTGACCGCCAACGGCGTCCTCCACCAGCCGCCGCAGAGCTCAGACGACGGCGAGGAGAAGCTGGTGGCCATGCAGCGCCGCGTGCTCGAGTCCATCGTCACCATGGTCCCGGGCGAGCCGGGGTCGGTCACCGGCCGCTTCCTACTAAGGCTGCTGCGGGTGGCCAACTACGTCAGCGCGTCGTCGTCCACGCGCGCGCAGCTGATCCGGCAGGCCGGCTCCCAGCTCGACGAGGCCAGGGCGGCGGACCTGCTCATCCCGCTGCCATCGGACCCGCACGCGTACGACGTCGGCACGGCGGAGGCCGTGCTCGAGCACTTCCTGGCCCAGTTCCAGCGGCCCGCGCCGACCGACGAGCGGCGTCGGATGAGCGCCGCCATGGAGAAGGTCGCCAGGATCTTCGACGAGTACCTGCGGACCATCGCGCTGGACCGCAGCTTCCCTGTCGGCAAGTTCGTTGACCTCGTCGAATGCTTGCCGGACATCGCCCGCAGTGACCACGACGGCCTCTACTACGCCATCGACATCTATCTCAAGGAGCACCCGGAGCTGAGCAAGGCGGATAAGAAGCGGTTGTGCCGGCTGATAGACTGCAGGAAGCTGTCGCCAGACGTGCGGGCGCAGGCGATAGCCAACGACCGGATGCCACTGCGCACCATTGTACAGCTTCTCTTCGTCGAGCAGGAGCGGACCATCGGTGCAGCCGCCGGCAGCCATGCTGCTTCCGGTGGCGCCGCAGATCGGGCCTCCGTCGGTGTTATCTCCCAGCTCGCGGCGGGAACCAAAGATGAAGAACCAGCCTCGTCTGCTGGCCACAAGTCAGACGTGCACAGACCACGCCGGGACCATGCCCGGGTAGCCGATGGAGCCTCTGCCATGGCGAGGTCGCTGTCGGCGTCAACCAAGACTCCACCGGCAGCGAGGAAGGAGAGGACGCCGGAGGAGACGGGGAGCAGGATGAGGAACAAGCAG
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