| TFome pUT6442 | |
|---|---|
| Template | BT066094.1 |
| Genbank Insert ID | KJ728172 |
| Protein Name | ZmWRKY72 |
| Species | Zea mays |
| TF Family | WRKY |
| Gene id | GRMZM5G816457 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT6442
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B362_F |
| Temperature (ºC) | 67.5 |
| Sequence
|
caccATGGAAGGTCATGTAGCAATGGA |
3' Primer
| Name | B362_R |
| Temperature (ºC) | |
| Sequence
|
CATCTGCTGGCGCAACCGC |
PCR Condition
PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer f
Amino Acid Sequence
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MEGHVAMEWNDPNPGPESLTSFQTEAVRPDTAGGHSNDDVEAGFEKHGLAVTVRPPEEEARSVPLTPQLGQKSSPGSSLAERMQARAGFMVSKLNMPFSTAAGADNSVPEVPSPYLTIPPGLSPATLLESPVFVSNSMGQASPTTGTLFMFGSTNDNDPIRFGGGPPSVGDGPNAFPFKPLDLKSSHYTAEAMKEQNTKSSVKTETKILTVQEASLLSQLNQLNHNSQTIINSGGPHDPKLSRPASGAGAGNEHISPPDHGQTAEESDAREDYPAMATTTPAEDGYSWRKYGQKQVKHSEYPRSYFKCTHPNCLVKKKVERSHEGHITEIIYKGAHNHPKPTQSRRPGVQPVHPFGDSAQADAADNLGSQANALDANQPRRAGVQDGMDATSSPSVPIERCDSPASMQVDSATRFGSPEGADVTSVSDEVGGDDRVTRGSMSQGGADAEGDELECKRRKLESYAIDMSTASRAVREPRVVIQTTSEVDILDDGYRWRKYGQKVVKGNPNPRSYYKCTYPGCVVRKHVERASHDLKSVITTYEGRHNHEVPAARNSGHPGTAAATGAGGPRRLEHPSLRDGLMGHLGGCGVPFGLPPPRDPLAPMGNYPTYASSIPLGGAGPTSLPSLPVPAGTLSAVDGLKLPMLAPSSLQQHPLLRHRQAMQAAGLVAPKDDVKVEGNVAGGAAPSVYQQMMCSGLRLRQQM
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGAAGGTCATGTAGCAATGGAGTGGAATGATCCTAATCCAGGCCCAGAATCCTTGACGAGCTTCCAAACCGAAGCTGTTCGACCAGACACCGCAGGGGGCCACAGCAACGACGACGTTGAAGCTGGATTTGAGAAGCATGGCTTGGCAGTCACCGTAAGGCCGCCTGAGGAGGAAGCTCGGTCAGTGCCACTAACCCCACAGCTTGGCCAGAAGTCCAGTCCTGGCAGCAGCCTCGCGGAGAGGATGCAGGCAAGAGCTGGATTCATGGTGTCGAAGCTCAACATGCCCTTCAGCACAGCAGCTGGAGCTGACAATTCAGTACCCGAAGTTCCATCGCCCTACCTCACGATTCCGCCTGGCCTGAGCCCAGCAACGCTGCTGGAGTCACCAGTCTTCGTTTCTAATTCCATGGGCCAGGCCTCGCCAACCACGGGGACGCTATTCATGTTTGGTAGCACAAATGACAATGATCCTATTAGATTTGGAGGTGGTCCTCCTTCAGTTGGAGATGGCCCTAATGCTTTTCCTTTCAAGCCCTTAGATCTGAAATCCTCACACTACACTGCTGAAGCAATGAAGGAACAAAATACGAAGTCTTCTGTCAAGACTGAAACCAAGATTCTGACAGTACAAGAGGCTAGTTTGCTCAGCCAACTGAACCAGCTGAATCACAATTCGCAGACCATTATTAATAGTGGCGGTCCTCATGACCCCAAGCTCAGCCGTCCTGCATCTGGCGCCGGTGCAGGCAATGAACACATATCACCCCCGGATCACGGACAGACAGCTGAGGAAAGTGATGCGAGGGAAGACTACCCGGCAATGGCTACTACCACGCCAGCAGAGGACGGGTACAGCTGGAGGAAGTACGGGCAGAAGCAGGTGAAGCACAGCGAGTACCCGCGTAGCTACTTCAAGTGCACGCATCCCAACTGCCTGGTGAAGAAGAAGGTCGAGCGCTCGCACGAGGGGCACATCACGGAGATCATCTACAAGGGCGCGCATAACCACCCGAAGCCCACGCAGAGCCGCCGTCCAGGCGTCCAGCCCGTGCACCCGTTTGGCGACAGTGCGCAGGCGGACGCAGCCGACAACCTGGGGTCACAGGCAAACGCACTGGACGCCAACCAGCCGAGGCGTGCGGGTGTTCAGGATGGCATGGACGCGACGTCGTCGCCCTCAGTTCCCATTGAGCGCTGTGACTCGCCGGCCTCGATGCAGGTCGACTCGGCAACTCGGTTTGGGTCCCCCGAGGGCGCGGATGTGACGTCTGTGTCCGACGAGGTGGGCGGGGACGACAGGGTGACTCGTGGCAGTATGTCTCAGGGCGGTGCCGACGCGGAGGGTGACGAGCTGGAATGTAAACGAAGGAAGCTGGAGTCGTACGCCATCGATATGAGCACCGCGTCGAGAGCTGTTCGCGAGCCTCGCGTGGTGATCCAGACGACGAGCGAGGTGGACATCCTAGATGACGGCTACCGCTGGCGCAAGTACGGGCAGAAGGTCGTCAAGGGCAACCCGAATCCAAGGAGCTACTACAAGTGCACGTACCCGGGCTGCGTGGTGCGGAAGCACGTGGAGCGCGCGTCGCACGACCTCAAGTCCGTGATCACCACGTACGAGGGCAGGCACAACCACGAGGTCCCCGCCGCCAGGAATAGCGGCCACCCGGGCACCGCGGCGGCGACAGGCGCGGGTGGCCCGCGCAGGCTGGAGCACCCGTCGCTGCGCGACGGCCTGATGGGGCACTTGGGCGGCTGCGGCGTGCCGTTCGGCCTGCCGCCGCCTAGGGATCCGCTGGCGCCGATGGGCAATTACCCGACGTATGCCTCCTCCATTCCCCTTGGCGGCGCCGGCCCCACGTCACTGCCGAGCCTGCCGGTGCCGGCGGGGACCCTCAGCGCCGTCGATGGGCTGAAGCTCCCGATGCTGGCGCCGTCGTCCCTGCAGCAGCACCCGCTGCTGAGGCACCGGCAGGCGATGCAGGCCGCTGGCCTCGTGGCGCCCAAGGACGACGTGAAGGTCGAGGGCAACGTTGCCGGCGGCGCTGCCCCGTCGGTGTACCAGCAGATGATGTGCAGCGGGCTGCGGTTGCGCCAGCAGATG
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