| TFome pUT6454 | |
|---|---|
| Template | BT064618 |
| Genbank Insert ID | KJ728182 |
| Protein Name | ZmOrphan334 |
| Species | Zea mays |
| TF Family | Orphans |
| Gene id | GRMZM2G081359 |
| transcript | T02 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT6454
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C534_F |
| Temperature (ºC) | 71.3 |
| Sequence
|
caccATGTCTTCACGCTCGGGGTCTACG |
3' Primer
| Name | C534_R |
| Temperature (ºC) | |
| Sequence
|
AGAGGACCAATCTTTAGTCTGGAAATC |
PCR Condition
Volumes per reaction (25l): 5 l GC buffer (Thermo
Amino Acid Sequence
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MSSRSGSTSGGSTGGGSSAGGGAAVPLALLLRREVASERTASERPELHSGLFSQAKKGEDFTFLKLECERVPGVPSSSFSAFGLFDGHNGNGAAIYTKENLLNNILNAVPADLNREDWLAALPRALVAAFVKTDKDFQTKDWSS
TFome clones do not contain stop codon at 3'end.
CDS
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ATGTCTTCACGCTCGGGGTCTACGAGCGGCGGGAGCACCGGTGGCGGCAGCAGCGCAGGGGGAGGCGCCGCGGTGCCGCTGGCCTTGCTGCTGAGGCGGGAGGTGGCCAGCGAGCGCACCGCATCGGAGCGACCGGAGCTGCACTCGGGCCTCTTCAGCCAGGCCAAGAAAGGCGAGGACTTCACATTCCTCAAGCTCGAATGCGAGCGCGTCCCCGGCGTCCCGTCCTCCTCCTTCTCCGCCTTCGGCCTGTTTGATGGGCACAATGGGAACGGGGCCGCAATTTACACCAAAGAGAATCTCTTGAACAACATCTTGAACGCGGTCCCTGCTGATCTCAACAGGGAAGACTGGCTTGCTGCACTCCCAAGAGCGCTGGTTGCTGCATTCGTTAAAACGGATAAAGATTTCCAGACTAAAGATTGGTCCTCT
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