| TFome pUT6461 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ728188 |
| Protein Name | ZmMADS8 |
| Species | Zea mays |
| TF Family | MADS |
| Gene id | GRMZM2G102161 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT6461
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | CD0123_F |
| Temperature (ºC) | 76.1 |
| Sequence
|
caccATGGGTCGCGGCAAGGTGGAGCTGA |
3' Primer
| Name | CD0123_R |
| Temperature (ºC) | |
| Sequence
|
TATCCATCCAGACCGACCGTGCTCG |
PCR Condition
PCR Conditions: Volumes per reaction (30l): 6 l GC
Amino Acid Sequence
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MGRGKVELKRIENKISRQVTFAKRRNGLLKKAYELSLLCDAEVALIIFSGRGRLFEFSSSSCMYKTLERYRSSNYSTQEVKAPLESEINYQDYLKLRTRVDFLQTTQRNILGEDLGPLSMKELEQLENQIEVSLKHIRSRKNQMLLDQLFDLKSKEQELQDLNKDLRKKLQETSPQNVIHDVSWEEGGHSGSSVLGPFQGLLQHHGNDPSLQTRYHQQAYMDQLNEDMADPDEHGRSGWI
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGGTCGCGGCAAGGTGGAGCTGAAGCGGATCGAGAACAAGATCAGCCGGCAGGTGACGTTCGCCAAGCGCAGGAACGGGCTGCTCAAGAAGGCGTACGAGCTGTCGCTGCTGTGCGACGCCGAGGTCGCGCTCATCATATTCTCCGGCCGCGGCCGCCTCTTCGAGTTCTCCAGCTCCTCATGCATGTACAAAACACTCGAGAGATACCGCAGCTCCAACTACAGCACACAGGAAGTAAAAGCTCCACTGGAGAGTGAAATTAACTACCAGGACTACTTGAAGTTGAGGACCAGAGTTGATTTTCTCCAGACTACACAAAGAAACATCCTTGGCGAAGATCTGGGCCCACTCAGCATGAAGGAGCTCGAGCAGCTGGAGAACCAGATAGAGGTATCCCTGAAGCATATCAGGTCAAGAAAGAATCAGATGCTACTCGACCAGCTCTTTGATCTCAAAAGCAAGGAACAAGAGTTGCAGGATCTCAACAAAGACCTAAGGAAAAAGCTGCAAGAAACAAGTCCGCAGAATGTGATCCACGACGTCTCCTGGGAGGAAGGCGGGCACAGTGGGAGTAGTGTTCTTGGTCCGTTCCAGGGACTCCTTCAGCACCATGGCAATGATCCTTCCCTGCAGACACGGTACCATCAGCAGGCCTACATGGACCAGCTGAACGAGGACATGGCGGACCCTGACGAGCACGGTCGGTCTGGATGGATA
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