| TFome pUT6469 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ728195 |
| Protein Name | ZmMYBR47 |
| Species | Zea mays |
| TF Family | MYB-related |
| Gene id | GRMZM2G117497 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT6469
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | CD0168_F |
| Temperature (ºC) | 74.2 |
| Sequence
|
caccATGTCCGCCTCACGCAGCTCCTCTC |
3' Primer
| Name | CD0168_R |
| Temperature (ºC) | |
| Sequence
|
GGTCCAGAATCCATGTGTCTTGTAC |
PCR Condition
PCR Conditions: Volumes per reaction (30l): 6 l GC
Amino Acid Sequence
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MSASRSSSPNSISQWSQKENKMFEEALAYYGEGTSNRWDKVSRAMGGIKSAEEVRCHYEDLDYDVKMIESGHVPYPKYKTHGFWT
TFome clones do not contain stop codon at 3'end.
CDS
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ATGTCCGCCTCACGCAGCTCCTCTCCCAACTCCATCTCACAGTGGAGCCAGAAAGAGAACAAGATGTTTGAAGAGGCACTCGCATACTACGGCGAGGGCACATCCAACCGGTGGGACAAGGTGTCCAGAGCCATGGGAGGCATCAAGTCTGCTGAGGAGGTGCGCTGCCACTATGAAGATCTTGACTATGACGTCAAGATGATTGAATCAGGCCACGTGCCGTACCCCAAGTACAAGACACATGGATTCTGGACC
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